0 19 Apoptosis-resistant apoptosis-resistant JJ 20 21 T t NN 22 27 cells cell NNS 28 32 have have VBP 33 34 a a DT 35 45 deficiency deficiency NN 46 48 in in IN 49 67 NF-kappaB-mediated nf-kappab-mediated JJ 68 77 induction induction NN 78 80 of of IN 81 84 Fas Fas NNP 85 91 ligand ligand NN 92 105 transcription transcription NN 105 106 . . . 108 117 Apoptosis Apoptosis NNP 118 125 induced induce VBN 126 133 through through IN 134 137 the the DT 138 141 TCR TCR NNP 142 144 in in IN 145 149 CD4+ cd4+ JJ 150 151 T t NN 152 157 cells cell NNS 158 160 is be VBZ 161 167 mostly mostly RB 168 176 mediated mediate VBN 177 179 by by IN 180 183 the the DT 184 193 inducible inducible JJ 194 204 expression expression NN 205 207 of of IN 208 211 Fas Fas NNP 212 218 ligand ligand NN 219 220 ( ( ( 220 224 FasL FasL NNP 224 225 ) ) ) 226 228 as as IN 229 230 a a DT 231 238 primary primary JJ 239 244 event event NN 245 252 leading lead VBG 253 255 to to TO 256 259 the the DT 260 270 commitment commitment NN 271 273 to to TO 274 279 death death NN 279 280 . . . 281 283 To to TO 284 288 gain gain VB 289 290 a a DT 291 297 better good JJR 298 311 understanding understanding NN 312 314 of of IN 315 318 the the DT 319 334 transcriptional transcriptional JJ 335 341 events event NNS 342 346 that that WDT 347 355 regulate regulate VBP 356 360 this this DT 361 371 expression expression NN 371 372 , , , 373 375 we we PRP 376 380 took take VBD 381 390 advantage advantage NN 391 393 of of IN 394 397 our our PRP$ 398 408 previously previously RB 409 418 described describe VBN 419 425 mutant mutant JJ 426 432 Jurkat Jurkat NNP 433 438 cells cell NNS 438 439 . . . 440 445 These these DT 446 451 cells cell NNS 452 455 are be VBP 456 465 deficient deficient JJ 466 468 in in IN 469 473 FasL FasL NNP 474 484 expression expression NN 485 488 and and CC 489 498 apoptosis apoptosis NN 499 506 induced induce VBN 507 511 upon upon IN 512 515 TCR TCR NNP 516 526 triggering triggering NN 526 527 , , , 528 536 although although IN 537 542 their their PRP$ 543 551 cytokine cytokine NN 552 553 ( ( ( 553 557 IL-2 IL-2 NNP 558 561 and and CC 562 571 IFN-gamma IFN-gamma NNP 571 572 ) ) ) 573 583 production production NN 584 586 is be VBZ 587 593 normal normal JJ 593 594 . . . 595 599 Here here RB 600 602 we we PRP 603 607 show show VBP 608 612 that that IN 613 617 both both CC 618 619 a a DT 620 625 FasL- fasl- NN 626 629 and and CC 630 631 a a DT 632 641 consensus consensus NN 642 652 NF-kappaB- nf-kappab- NN 653 661 reporter reporter NN 662 671 construct construct NN 672 675 are be VBP 676 689 inefficiently inefficiently RB 690 697 induced induce VBN 698 700 in in IN 701 706 these these DT 707 712 cells cell NNS 713 721 compared compare VBN 722 724 to to TO 725 734 wild-type wild-type JJ 735 740 cells cell NNS 740 741 . . . 742 744 In in IN 745 753 addition addition NN 753 754 , , , 755 757 we we PRP 758 769 demonstrate demonstrate VBP 770 774 that that IN 775 778 the the DT 779 788 inducible inducible JJ 789 804 transcriptional transcriptional JJ 805 813 activity activity NN 814 816 of of IN 817 820 the the DT 821 825 FasL FasL NNP 826 834 reporter reporter NN 835 837 is be VBZ 838 847 abolished abolish VBN 848 850 by by IN 851 859 specific specific JJ 860 870 inhibitors inhibitor NNS 871 873 of of IN 874 883 NF-kappaB NF-kappaB NNP 884 894 activation activation NN 894 895 . . . 896 900 Thus thus RB 900 901 , , , 902 904 we we PRP 905 910 could could MD 911 916 trace trace VB 917 920 the the DT 921 928 deficit deficit NN 929 931 of of IN 932 935 the the DT 936 942 mutant mutant JJ 943 948 cells cell NNS 949 951 to to TO 952 954 an an DT 955 966 inefficient inefficient JJ 967 976 NF-kappaB nf-kappab NN 977 987 activation activation NN 987 988 , , , 989 999 evidencing evidence VBG 1000 1001 a a DT 1002 1010 relevant relevant JJ 1011 1015 role role NN 1016 1019 for for IN 1020 1029 NF-kappaB NF-kappaB NNP 1030 1032 in in IN 1033 1036 the the DT 1037 1047 regulation regulation NN 1048 1050 of of IN 1051 1055 FasL FasL NNP 1056 1066 expression expression NN 1067 1069 in in IN 1070 1079 activated activate VBN 1080 1081 T t NN 1082 1087 cells cell NNS 1087 1088 . . . 1089 1100 Furthermore furthermore RB 1100 1101 , , , 1102 1105 our our PRP$ 1106 1113 results result NNS 1114 1121 suggest suggest VBP 1122 1126 that that IN 1127 1130 the the DT 1131 1140 induction induction NN 1141 1143 of of IN 1144 1148 FasL FasL NNP 1149 1155 versus versus CC 1156 1164 cytokine cytokine NN 1165 1169 gene gene NN 1170 1180 expression expression NN 1181 1183 is be VBZ 1184 1198 differentially differentially RB 1199 1208 sensitive sensitive JJ 1209 1211 to to TO 1212 1221 NF-kappaB NF-kappaB NNP 1222 1233 deprivation deprivation NN 1233 1234 . . .