0 9 NF-kappaB NF-kappaB NNP 10 19 regulates regulate VBZ 20 35 Fas/APO-1/CD95- fas/apo-1/cd95- NN 36 39 and and CC 40 44 TCR- tcr- NN 45 53 mediated mediate VBN 54 63 apoptosis apoptosis NN 64 66 of of IN 67 68 T t NN 69 80 lymphocytes lymphocyte NNS 80 81 . . . 83 86 The the DT 87 98 maintenance maintenance NN 99 101 of of IN 102 112 lymphocyte lymphocyte NN 113 124 homeostasis homeostasis NN 125 127 by by IN 128 137 apoptosis apoptosis NN 138 140 is be VBZ 141 142 a a DT 143 151 critical critical JJ 152 162 regulatory regulatory JJ 163 172 mechanism mechanism NN 173 175 in in IN 176 179 the the DT 180 186 normal normal JJ 187 193 immune immune JJ 194 200 system system NN 200 201 . . . 202 205 The the DT 206 219 transcription transcription NN 220 226 factor factor NN 227 236 NF-kappaB NF-kappaB NNP 237 240 has have VBZ 241 245 been be VBN 246 251 shown show VBN 252 254 to to TO 255 259 play play VB 260 261 a a DT 262 266 role role NN 267 269 in in IN 270 280 protecting protect VBG 281 286 cells cell NNS 287 294 against against IN 295 300 death death NN 301 309 mediated mediate VBN 310 312 by by IN 313 316 TNF TNF NNP 317 319 We we PRP 320 324 show show VBP 325 329 here here RB 330 334 that that IN 335 344 NF-kappaB NF-kappaB NNP 345 349 also also RB 350 353 has have VBZ 354 355 a a DT 356 360 role role NN 361 363 in in IN 364 374 regulating regulate VBG 375 398 Fas/APO-1/CD95-mediated fas/apo-1/cd95-mediated JJ 399 404 death death NN 404 405 , , , 406 407 a a DT 408 413 major major JJ 414 421 pathway pathway NN 422 424 of of IN 425 435 peripheral peripheral JJ 436 437 T t NN 438 442 cell cell NN 443 448 death death NN 448 449 . . . 450 462 Transfection Transfection NNP 463 465 of of IN 466 472 Jurkat Jurkat NNP 473 478 cells cell NNS 479 483 with with IN 484 487 the the DT 488 497 NF-kappaB NF-kappaB NNP 498 506 subunits subunit NNS 507 510 p50 p50 NN 511 514 and and CC 515 518 p65 p65 NN 519 526 confers confer VBZ 527 537 resistance resistance NN 538 545 against against IN 546 558 Fas-mediated fas-mediated JJ 559 568 apoptosis apoptosis NN 568 569 . . . 570 582 Reciprocally reciprocally RB 582 583 , , , 584 594 inhibition inhibition NN 595 597 of of IN 598 607 NF-kappaB NF-kappaB NNP 608 618 activation activation NN 619 621 by by IN 622 623 a a DT 624 631 soluble soluble JJ 632 639 peptide peptide NN 640 649 inhibitor inhibitor NN 650 652 or or CC 653 654 a a DT 655 663 dominant dominant JJ 664 668 form form NN 669 671 of of IN 672 675 the the DT 676 685 NF-kappaB NF-kappaB NNP 686 695 inhibitor inhibitor NN 695 696 , , , 697 704 IkappaB IkappaB NNP 704 705 , , , 706 711 makes make VBZ 712 715 the the DT 716 721 cells cell NNS 722 726 more more RBR 727 738 susceptible susceptible JJ 739 741 to to TO 742 754 Fas-mediated fas-mediated JJ 755 764 apoptosis apoptosis NN 764 765 . . . 766 777 Furthermore furthermore RB 777 778 , , , 779 789 inhibition inhibition NN 790 792 of of IN 793 802 NF-kappaB NF-kappaB NNP 803 813 activation activation NN 814 816 by by IN 817 818 a a DT 819 826 soluble soluble JJ 827 834 peptide peptide NN 835 844 inhibitor inhibitor NN 845 853 rendered render VBD 854 855 a a DT 856 857 T t NN 858 862 cell cell NN 863 872 hybridoma hybridoma NN 873 877 more more RBR 878 889 susceptible susceptible JJ 890 892 to to TO 893 905 TCR-mediated tcr-mediated JJ 906 915 apoptosis apoptosis NN 915 916 . . . 917 932 Correspondingly correspondingly RB 932 933 , , , 934 946 transfection transfection NN 947 949 of of IN 950 953 p50 p50 NN 954 957 and and CC 958 961 p65 p65 NN 962 970 provided provide VBD 971 983 considerable considerable JJ 984 994 protection protection NN 995 999 from from IN 1000 1012 TCR-mediated tcr-mediated JJ 1013 1022 apoptosis apoptosis NN 1022 1023 . . . 1024 1029 These these DT 1030 1042 observations observation NNS 1043 1047 were be VBD 1048 1060 corroborated corroborate VBN 1061 1063 by by IN 1064 1071 studies study NNS 1072 1074 on on IN 1075 1087 Fas-mediated fas-mediated JJ 1088 1093 death death NN 1094 1096 in in IN 1097 1104 primary primary JJ 1105 1106 T t NN 1107 1112 cells cell NNS 1112 1113 . . . 1114 1126 Concanavalin Concanavalin NNP 1127 1138 A-activated a-activated JJ 1139 1146 cycling cycling NN 1147 1148 T t NN 1149 1153 cell cell NN 1154 1160 blasts blast NNS 1161 1165 from from IN 1166 1170 mice mouse NNS 1171 1175 that that WDT 1176 1179 are be VBP 1180 1190 transgenic transgenic JJ 1191 1194 for for IN 1195 1198 the the DT 1199 1207 dominant dominant JJ 1208 1215 IkappaB IkappaB NNP 1216 1224 molecule molecule NN 1225 1229 have have VBP 1230 1239 increased increase VBN 1240 1251 sensitivity sensitivity NN 1252 1254 to to TO 1255 1267 Fas-mediated fas-mediated JJ 1268 1277 apoptosis apoptosis NN 1277 1278 , , , 1279 1289 associated associate VBN 1290 1294 with with IN 1295 1296 a a DT 1297 1312 down-regulation down-regulation NN 1313 1315 of of IN 1316 1325 NF-kappaB NF-kappaB NNP 1326 1335 complexes complex NNS 1336 1338 in in IN 1339 1342 the the DT 1343 1350 nucleus nucleus NN 1350 1351 . . . 1352 1354 In in IN 1355 1363 addition addition NN 1363 1364 , , , 1365 1373 blocking block VBG 1374 1377 TNF TNF NNP 1377 1378 , , , 1379 1385 itself itself PRP 1386 1387 a a DT 1388 1396 positive positive JJ 1397 1406 regulator regulator NN 1407 1409 of of IN 1410 1419 NF-kappaB NF-kappaB NNP 1419 1420 , , , 1421 1425 with with IN 1426 1438 neutralizing neutralize VBG 1439 1449 antibodies antibody NNS 1450 1457 renders render VBZ 1458 1461 the the DT 1462 1467 cells cell NNS 1468 1472 more more RBR 1473 1484 susceptible susceptible JJ 1485 1487 to to TO 1488 1505 anti-Fas-mediated anti-fas-mediated JJ 1506 1515 apoptosis apoptosis NN 1515 1516 . . . 1517 1519 In in IN 1520 1527 summary summary NN 1527 1528 , , , 1529 1532 our our PRP$ 1533 1540 results result NNS 1541 1548 provide provide VBP 1549 1559 compelling compelling JJ 1560 1568 evidence evidence NN 1569 1573 that that IN 1574 1583 NF-kappaB NF-kappaB NNP 1584 1592 protects protect VBZ 1593 1600 against against IN 1601 1613 Fas-mediated fas-mediated JJ 1614 1619 death death NN 1620 1623 and and CC 1624 1626 is be VBZ 1627 1633 likely likely JJ 1634 1636 to to TO 1637 1639 be be VB 1640 1642 an an DT 1643 1652 important important JJ 1653 1662 regulator regulator NN 1663 1665 of of IN 1666 1667 T t NN 1668 1672 cell cell NN 1673 1684 homeostasis homeostasis NN 1685 1688 and and CC 1689 1698 tolerance tolerance NN 1698 1699 . . .