0	8	Evidence	evidence	NN
9	12	for	for	IN
13	21	distinct	distinct	JJ
22	35	intracellular	intracellular	JJ
36	45	signaling	signaling	NN
46	54	pathways	pathway	NNS
55	57	in	in	IN
58	63	CD34+	cd34+	JJ
64	74	progenitor	progenitor	NN
75	77	to	to	TO
78	87	dendritic	dendritic	JJ
88	92	cell	cell	NN
93	108	differentiation	differentiation	NN
109	113	from	from	IN
114	115	a	a	DT
116	121	human	human	JJ
122	126	cell	cell	NN
127	131	line	line	NN
132	137	model	model	NN
137	138	.	.	.

140	153	Intracellular	intracellular	JJ
154	161	signals	signal	NNS
162	166	that	that	WDT
167	174	mediate	mediate	VBP
175	190	differentiation	differentiation	NN
191	193	of	of	IN
194	205	pluripotent	pluripotent	JJ
206	217	hemopoietic	hemopoietic	JJ
218	229	progenitors	progenitor	NNS
230	232	to	to	TO
233	242	dendritic	dendritic	JJ
243	248	cells	cell	NNS
249	250	(	(	(
250	252	DC	DC	NNP
252	253	)	)	)
254	257	are	be	VBP
258	265	largely	largely	RB
266	275	undefined	undefined	JJ
275	276	.	.	.

277	279	We	we	PRP
280	284	have	have	VBP
285	295	previously	previously	RB
296	301	shown	show	VBN
302	306	that	that	IN
307	314	protein	protein	NN
315	321	kinase	kinase	NNP
322	323	C	C	NNP
324	325	(	(	(
325	328	PKC	PKC	NNP
328	329	)	)	)
330	340	activation	activation	NN
341	342	(	(	(
342	346	with	with	IN
347	354	phorbol	phorbol	NN
355	360	ester	ester	NN
361	362	(	(	(
362	365	PMA	PMA	NNP
365	366	)	)	)
367	372	alone	alone	RB
372	373	)	)	)
374	386	specifically	specifically	RB
387	394	induces	induce	VBZ
395	410	differentiation	differentiation	NN
411	413	of	of	IN
414	421	primary	primary	JJ
422	427	human	human	JJ
428	433	CD34+	cd34+	JJ
434	445	hemopoietic	hemopoietic	JJ
446	456	progenitor	progenitor	NN
457	462	cells	cell	NNS
463	464	(	(	(
464	467	HPC	HPC	NNP
467	468	)	)	)
469	471	to	to	TO
472	478	mature	mature	JJ
479	481	DC	DC	NNP
481	482	.	.	.

483	485	We	we	PRP
486	489	now	now	RB
490	494	find	find	VBP
495	499	that	that	IN
500	515	cytokine-driven	cytokine-driven	JJ
516	517	(	(	(
517	539	granulocyte-macrophage	granulocyte-macrophage	JJ
540	543	CSF	CSF	NNP
544	547	and	and	CC
548	557	TNF-alpha	TNF-alpha	NNP
557	558	)	)	)
559	564	CD34+	cd34+	JJ
565	573	HPC–>DC	HPC–>DC	NNP
574	589	differentiation	differentiation	NN
590	592	is	be	VBZ
593	607	preferentially	preferentially	RB
608	615	blocked	block	VBN
616	618	by	by	IN
619	629	inhibitors	inhibitor	NNS
630	632	of	of	IN
633	636	PKC	PKC	NNP
637	647	activation	activation	NN
647	648	.	.	.

649	651	To	to	TO
652	659	further	further	RB
660	668	identify	identify	VB
669	682	intracellular	intracellular	JJ
683	690	signals	signal	NNS
691	694	and	and	CC
695	705	downstream	downstream	JJ
706	712	events	event	NNS
713	722	important	important	JJ
723	725	in	in	IN
726	731	CD34+	cd34+	JJ
732	740	HPC–>DC	HPC–>DC	NNP
741	756	differentiation	differentiation	NN
757	759	we	we	PRP
760	764	have	have	VBP
765	778	characterized	characterize	VBN
779	780	a	a	DT
781	786	human	human	JJ
787	795	leukemic	leukemic	JJ
796	800	cell	cell	NN
801	805	line	line	NN
806	811	model	model	NN
812	814	of	of	IN
815	819	this	this	DT
820	827	process	process	NN
827	828	.	.	.

829	832	The	the	DT
833	838	CD34+	cd34+	JJ
839	853	myelomonocytic	myelomonocytic	JJ
854	858	cell	cell	NN
859	863	line	line	NN
864	867	KG1	kg1	NN
868	882	differentiates	differentiate	VBZ
883	887	into	into	IN
888	902	dendritic-like	dendritic-like	JJ
903	908	cells	cell	NNS
909	911	in	in	IN
912	920	response	response	NN
921	923	to	to	TO
924	946	granulocyte-macrophage	granulocyte-macrophage	JJ
947	950	CSF	CSF	NNP
951	955	plus	plus	CC
956	965	TNF-alpha	TNF-alpha	NNP
965	966	,	,	,
967	969	or	or	CC
970	973	PMA	PMA	NNP
974	975	(	(	(
975	979	with	with	IN
980	982	or	or	CC
983	990	without	without	IN
991	994	the	the	DT
995	1002	calcium	calcium	NN
1003	1012	ionophore	ionophore	NN
1013	1022	ionomycin	ionomycin	NN
1022	1023	,	,	,
1024	1026	or	or	CC
1027	1036	TNF-alpha	TNF-alpha	NNP
1036	1037	)	)	)
1037	1038	,	,	,
1039	1043	with	with	IN
1044	1053	different	different	JJ
1054	1061	stimuli	stimulus	NNS
1062	1071	mediating	mediate	VBG
1072	1081	different	different	JJ
1082	1089	aspects	aspect	NNS
1090	1092	of	of	IN
1093	1096	the	the	DT
1097	1104	process	process	NN
1104	1105	.	.	.

1106	1116	Phenotypic	phenotypic	JJ
1117	1119	DC	DC	NNP
1120	1135	characteristics	characteristic	NNS
1136	1138	of	of	IN
1139	1142	KG1	kg1	NN
1143	1157	dendritic-like	dendritic-like	JJ
1158	1163	cells	cell	NNS
1164	1171	include	include	VBP
1172	1182	morphology	morphology	NN
1183	1184	(	(	(
1184	1191	loosely	loosely	RB
1192	1200	adherent	adherent	JJ
1201	1206	cells	cell	NNS
1207	1211	with	with	IN
1212	1216	long	long	JJ
1217	1224	neurite	neurite	NN
1225	1234	processes	process	NNS
1234	1235	)	)	)
1235	1236	,	,	,
1237	1240	MHC	MHC	NNP
1241	1247	I+/MHC	I+/MHC	NNP
1248	1274	IIbright/CD83+/CD86+/CD14-	IIbright/CD83+/CD86+/CD14-	NNP
1275	1282	surface	surface	NN
1283	1285	Ag	ag	NN
1286	1296	expression	expression	NN
1296	1297	,	,	,
1298	1301	and	and	CC
1302	1306	RelB	RelB	NNP
1307	1310	and	and	CC
1311	1317	DC-CK1	dc-ck1	NN
1318	1322	gene	gene	NN
1323	1333	expression	expression	NN
1333	1334	.	.	.

1335	1345	Functional	functional	JJ
1346	1348	DC	DC	NNP
1349	1364	characteristics	characteristic	NNS
1365	1372	include	include	VBP
1373	1378	fluid	fluid	NN
1379	1384	phase	phase	NN
1385	1398	macromolecule	macromolecule	NN
1399	1405	uptake	uptake	NN
1406	1407	(	(	(
1407	1419	FITC-dextran	FITC-dextran	NNP
1419	1420	)	)	)
1421	1424	and	and	CC
1425	1435	activation	activation	NN
1436	1438	of	of	IN
1439	1446	resting	rest	VBG
1447	1448	T	t	NN
1449	1454	cells	cell	NNS
1454	1455	.	.	.

1456	1466	Comparison	comparison	NN
1467	1469	of	of	IN
1470	1473	KG1	kg1	NN
1474	1476	to	to	TO
1477	1480	the	the	DT
1481	1497	PMA-unresponsive	pma-unresponsive	JJ
1498	1505	subline	subline	NN
1506	1510	KG1a	KG1a	NNP
1511	1518	reveals	reveal	VBZ
1519	1530	differences	difference	NNS
1531	1533	in	in	IN
1534	1544	expression	expression	NN
1545	1547	of	of	IN
1548	1551	TNF	TNF	NNP
1552	1561	receptors	receptor	NNS
1562	1563	1	1	CD
1564	1567	and	and	CC
1568	1569	2	2	CD
1569	1570	;	;	:
1571	1574	PKC	PKC	NNP
1575	1583	isoforms	isoform	NNS
1584	1589	alpha	alpha	NN
1589	1590	,	,	,
1591	1595	beta	beta	NN
1596	1597	I	i	CD
1597	1598	,	,	,
1599	1603	beta	beta	NN
1604	1606	II	ii	CD
1606	1607	,	,	,
1608	1611	and	and	CC
1612	1614	mu	mu	NN
1614	1615	;	;	:
1616	1619	and	and	CC
1620	1624	RelB	RelB	NNP
1624	1625	,	,	,
1626	1636	suggesting	suggest	VBG
1637	1641	that	that	IN
1642	1647	these	these	DT
1648	1667	components/pathways	components/pathway	NNS
1668	1671	are	be	VBP
1672	1681	important	important	JJ
1682	1685	for	for	IN
1686	1688	DC	DC	NNP
1689	1704	differentiation	differentiation	NN
1704	1705	.	.	.

1706	1714	Together	together	RB
1714	1715	,	,	,
1716	1721	these	these	DT
1722	1730	findings	finding	NNS
1731	1742	demonstrate	demonstrate	VBP
1743	1747	that	that	IN
1748	1756	cytokine	cytokine	NN
1757	1759	or	or	CC
1760	1767	phorbol	phorbol	NN
1768	1773	ester	ester	NN
1774	1785	stimulation	stimulation	NN
1786	1788	of	of	IN
1789	1792	KG1	KG1	NNP
1793	1795	is	be	VBZ
1796	1797	a	a	DT
1798	1803	model	model	NN
1804	1806	of	of	IN
1807	1812	human	human	JJ
1813	1818	CD34+	cd34+	JJ
1819	1822	HPC	HPC	NNP
1823	1825	to	to	TO
1826	1828	DC	DC	NNP
1829	1844	differentiation	differentiation	NN
1845	1848	and	and	CC
1849	1856	suggest	suggest	VBP
1857	1861	that	that	IN
1862	1870	specific	specific	JJ
1871	1884	intracellular	intracellular	JJ
1885	1894	signaling	signaling	NN
1895	1903	pathways	pathway	NNS
1904	1911	mediate	mediate	VBP
1912	1920	specific	specific	JJ
1921	1927	events	event	NNS
1928	1930	in	in	IN
1931	1933	DC	DC	NNP
1934	1941	lineage	lineage	NN
1942	1952	commitment	commitment	NN
1952	1953	.	.	.