0 12 Differential differential JJ 13 23 regulation regulation NN 24 26 of of IN 27 33 4E-BP1 4E-BP1 NNP 34 37 and and CC 38 44 4E-BP2 4E-BP2 NNP 44 45 , , , 46 49 two two CD 50 60 repressors repressor NNS 61 63 of of IN 64 75 translation translation NN 76 86 initiation initiation NN 86 87 , , , 88 94 during during IN 95 100 human human JJ 101 108 myeloid myeloid JJ 109 113 cell cell NN 114 129 differentiation differentiation NN 129 130 . . . 132 137 Human human JJ 138 145 myeloid myeloid JJ 146 161 differentiation differentiation NN 162 164 is be VBZ 165 176 accompanied accompany VBN 177 179 by by IN 180 181 a a DT 182 190 decrease decrease NN 191 193 in in IN 194 198 cell cell NN 199 212 proliferation proliferation NN 212 213 . . . 214 221 Because because IN 222 225 the the DT 226 237 translation translation NN 238 242 rate rate NN 243 245 is be VBZ 246 248 an an DT 249 258 important important JJ 259 270 determinant determinant NN 271 273 of of IN 274 278 cell cell NN 279 292 proliferation proliferation NN 292 293 , , , 294 296 we we PRP 297 301 have have VBP 302 314 investigated investigate VBN 315 326 translation translation NN 327 337 initiation initiation NN 338 344 during during IN 345 350 human human JJ 351 358 myeloid myeloid JJ 359 363 cell cell NN 364 379 differentiation differentiation NN 380 385 using use VBG 386 389 the the DT 390 395 HL-60 HL-60 NNP 396 409 promyelocytic promyelocytic JJ 410 418 leukemia leukemia NN 419 423 cell cell NN 424 428 line line NN 429 432 and and CC 433 436 the the DT 437 442 U-937 u-937 NN 443 454 monoblastic monoblastic JJ 455 459 cell cell NN 460 464 line line NN 464 465 . . . 466 467 A a DT 468 476 decrease decrease NN 477 479 in in IN 480 483 the the DT 484 495 translation translation NN 496 500 rate rate NN 501 503 is be VBZ 504 512 observed observe VBN 513 517 when when WRB 518 521 the the DT 522 527 cells cell NNS 528 531 are be VBP 532 539 induced induce VBN 540 542 to to TO 543 556 differentiate differentiate VB 557 562 along along IN 563 566 the the DT 567 587 monocytic/macrophage monocytic/macrophage NN 588 595 pathway pathway NN 596 598 or or CC 599 604 along along IN 605 608 the the DT 609 621 granulocytic granulocytic JJ 622 629 pathway pathway NN 629 630 . . . 631 634 The the DT 635 645 inhibition inhibition NN 646 648 in in IN 649 656 protein protein NN 657 666 synthesis synthesis NN 667 677 correlates correlate VBZ 678 682 with with IN 683 691 specific specific JJ 692 702 regulation regulation NN 703 705 of of IN 706 709 two two CD 710 720 repressors repressor NNS 721 723 of of IN 724 735 translation translation NN 736 746 initiation initiation NN 746 747 , , , 748 754 4E-BP1 4E-BP1 NNP 755 758 and and CC 759 765 4E-BP2 4E-BP2 NNP 765 766 . . . 767 776 Induction Induction NNP 777 779 of of IN 780 785 HL-60 HL-60 NNP 786 789 and and CC 790 795 U-937 U-937 NNP 796 800 cell cell NN 801 816 differentiation differentiation NN 817 821 into into IN 822 843 monocytes/macrophages monocytes/macrophage NNS 844 846 by by IN 847 856 IFN-gamma IFN-gamma NNP 857 859 or or CC 860 863 PMA PMA NNP 864 871 results result VBZ 872 874 in in IN 875 876 a a DT 877 894 dephosphorylation dephosphorylation NN 895 898 and and CC 899 909 consequent consequent JJ 910 920 activation activation NN 921 923 of of IN 924 930 4E-BP1 4E-BP1 NNP 930 931 . . . 932 949 Dephosphorylation dephosphorylation NN 950 952 of of IN 953 959 4E-BP1 4E-BP1 NNP 960 963 was be VBD 964 968 also also RB 969 977 observed observe VBN 978 982 when when WRB 983 988 U-937 u-937 NN 989 994 cells cell NNS 995 999 were be VBD 1000 1007 induced induce VBN 1008 1010 to to TO 1011 1024 differentiate differentiate VB 1025 1029 into into IN 1030 1051 monocytes/macrophages monocytes/macrophage NNS 1052 1061 following follow VBG 1062 1071 treatment treatment NN 1072 1076 with with IN 1077 1085 retinoic retinoic JJ 1086 1090 acid acid NN 1091 1093 or or CC 1094 1098 DMSO DMSO NNP 1098 1099 . . . 1100 1102 In in IN 1103 1111 contrast contrast NN 1111 1112 , , , 1113 1122 treatment treatment NN 1123 1125 of of IN 1126 1131 HL-60 hl-60 NN 1132 1137 cells cell NNS 1138 1142 with with IN 1143 1151 retinoic retinoic JJ 1152 1156 acid acid NN 1157 1159 or or CC 1160 1164 DMSO DMSO NNP 1164 1165 , , , 1166 1171 which which WDT 1172 1179 results result VBZ 1180 1182 in in IN 1183 1184 a a DT 1185 1197 granulocytic granulocytic JJ 1198 1213 differentiation differentiation NN 1214 1216 of of IN 1217 1222 these these DT 1223 1228 cells cell NNS 1228 1229 , , , 1230 1239 decreases decrease VBZ 1240 1246 4E-BP1 4e-bp1 NN 1247 1253 amount amount NN 1254 1261 without without IN 1262 1271 affecting affect VBG 1272 1275 its its PRP$ 1276 1291 phosphorylation phosphorylation NN 1292 1295 and and CC 1296 1304 strongly strongly RB 1305 1314 increases increase VBZ 1315 1321 4E-BP2 4e-bp2 NN 1322 1328 amount amount NN 1328 1329 . . . 1330 1335 Taken take VBN 1336 1344 together together RB 1344 1345 , , , 1346 1351 these these DT 1352 1356 data datum NNS 1357 1364 provide provide VBP 1365 1373 evidence evidence NN 1374 1377 for for IN 1378 1390 differential differential JJ 1391 1401 regulation regulation NN 1402 1404 of of IN 1405 1408 the the DT 1409 1422 translational translational JJ 1423 1432 machinery machinery NN 1433 1439 during during IN 1440 1445 human human JJ 1446 1453 myeloid myeloid JJ 1454 1469 differentiation differentiation NN 1469 1470 , , , 1471 1479 specific specific JJ 1480 1482 to to TO 1483 1486 the the DT 1487 1507 monocytic/macrophage monocytic/macrophage NN 1508 1515 pathway pathway NN 1516 1518 or or CC 1519 1521 to to TO 1522 1525 the the DT 1526 1538 granulocytic granulocytic JJ 1539 1546 pathway pathway NN 1546 1547 . . .