0 4 High high JJ 5 14 molecular molecular JJ 15 21 weight weight NN 22 29 dextran dextran NN 30 37 sulfate sulfate NN 38 47 increases increase VBZ 48 51 the the DT 52 60 activity activity NN 61 63 of of IN 64 83 NF-kappaB-regulated nf-kappab-regulated JJ 84 92 promoter promoter NN 93 95 in in IN 96 112 monocyte-derived monocyte-derived JJ 113 124 macrophages macrophage NNS 124 125 . . . 127 129 It it PRP 130 132 is be VBZ 133 138 known know VBN 139 143 that that IN 144 152 sulfated sulfated JJ 153 168 polysaccharides polysaccharide NNS 169 172 can can MD 173 178 mimic mimic VB 179 182 the the DT 183 189 action action NN 190 192 of of IN 193 199 common common JJ 200 206 T-cell t-cell NN 207 215 mitogens mitogen NNS 215 216 . . . 217 219 To to TO 220 231 investigate investigate VB 232 235 the the DT 236 245 molecular molecular JJ 246 251 basis basis NN 252 254 of of IN 255 258 the the DT 259 268 mitogenic mitogenic JJ 269 275 effect effect NN 276 278 of of IN 279 283 high high JJ 284 293 molecular molecular JJ 294 300 weight weight NN 301 308 dextran dextran NN 309 316 sulfate sulfate NN 317 318 ( ( ( 318 322 HMDS HMDS NNP 322 323 ) ) ) 323 324 , , , 325 341 monocyte-derived monocyte-derived JJ 342 353 macrophages macrophage NNS 354 358 were be VBD 359 370 transfected transfecte VBN 371 375 with with IN 376 387 recombinant recombinant JJ 388 395 plasmid plasmid NN 396 406 containing contain VBG 407 422 chloramphenicol chloramphenicol NN 423 429 acetyl acetyl NN 430 441 transferase transferase NN 442 443 ( ( ( 443 446 CAT CAT NNP 446 447 ) ) ) 448 456 reporter reporter NN 457 461 gene gene NN 462 467 under under IN 468 471 the the DT 472 479 control control NN 480 482 of of IN 483 486 the the DT 487 492 HIV-1 HIV-1 NNP 493 497 long long JJ 498 506 terminal terminal JJ 507 513 repeat repeat NN 514 515 ( ( ( 515 518 LTR LTR NNP 518 519 ) ) ) 520 528 promoter promoter NN 528 529 , , , 530 535 which which WDT 536 538 is be VBZ 539 548 regulated regulate VBN 549 551 by by IN 552 565 transcription transcription NN 566 572 factor factor NN 573 582 NF-kappaB nf-kappab NN 582 583 . . . 584 586 We we PRP 587 595 observed observe VBD 596 600 that that IN 601 605 HMDS HMDS NNP 605 606 , , , 607 614 similar similar JJ 615 617 to to TO 618 627 bacterial bacterial JJ 628 646 lipopolysaccharide lipopolysaccharide NN 647 648 ( ( ( 648 651 LPS LPS NNP 651 652 ) ) ) 652 653 , , , 654 663 increases increase VBZ 664 667 the the DT 668 678 expression expression NN 679 681 of of IN 682 685 CAT CAT NNP 686 694 reporter reporter NN 695 699 gene gene NN 700 710 suggesting suggest VBG 711 720 increased increase VBN 721 729 activity activity NN 730 732 of of IN 733 742 NF-kappaB NF-kappaB NNP 742 743 . . . 744 747 The the DT 748 758 activation activation NN 759 761 of of IN 762 771 NF-kappaB NF-kappaB NNP 772 782 correlated correlate VBD 783 787 with with IN 788 791 the the DT 792 801 increased increase VBN 802 812 expression expression NN 813 815 of of IN 816 820 B7.1 b7.1 NN 821 830 molecules molecule NNS 830 831 . . . 832 834 It it PRP 835 838 was be VBD 839 849 postulated postulate VBN 850 854 that that IN 855 859 this this DT 860 879 NF-kappaB-regulated nf-kappab-regulated JJ 880 888 promoter promoter NN 889 894 might might MD 895 899 play play VB 900 901 a a DT 902 906 role role NN 907 909 in in IN 910 913 the the DT 914 924 activation activation NN 925 927 of of IN 928 931 the the DT 932 941 accessory accessory JJ 942 947 cells cell NNS 948 950 as as RB 951 955 well well RB 956 958 as as IN 959 962 the the DT 963 967 rate rate NN 968 970 of of IN 971 982 replication replication NN 983 985 of of IN 986 991 HIV-1 HIV-1 NNP 992 994 in in IN 995 1011 monocyte-derived monocyte-derived JJ 1012 1023 macrophages macrophage NNS 1023 1024 . . .