0 10 Activation Activation NNP 11 13 of of IN 14 17 the the DT 18 23 Janus Janus NNP 24 30 kinase kinase NNP 31 39 3-STAT5a 3-STAT5a NNP 40 47 pathway pathway NN 48 53 after after IN 54 58 CD40 cd40 NN 59 69 triggering triggering NN 70 72 of of IN 73 78 human human JJ 79 88 monocytes monocyte NNS 89 92 but but CC 93 96 not not RB 97 99 of of IN 100 107 resting rest VBG 108 109 B B NNP 110 115 cells cell NNS 115 116 . . . 118 127 CD40/CD40 CD40/CD40 NNP 128 134 ligand ligand NN 135 147 interactions interaction NNS 148 152 play play VBP 153 154 a a DT 155 158 key key JJ 159 163 role role NN 164 166 in in IN 167 170 the the DT 171 177 immune immune JJ 178 187 responses response NNS 188 190 of of IN 191 192 B B NNP 193 204 lymphocytes lymphocyte NNS 204 205 , , , 206 215 monocytes monocyte NNS 215 216 , , , 217 220 and and CC 221 230 dendritic dendritic JJ 231 236 cells cell NNS 236 237 . . . 238 241 The the DT 242 248 signal signal NN 249 261 transduction transduction NN 262 268 events event NNS 269 278 triggered trigger VBN 279 281 by by IN 282 295 cross-linking cross-linking NN 296 298 of of IN 299 302 the the DT 303 307 CD40 cd40 NN 308 316 receptor receptor NN 317 321 have have VBP 322 326 been be VBN 327 333 widely widely RB 334 341 studied study VBN 342 344 in in IN 345 346 B B NNP 347 351 cell cell NN 352 357 lines line NNS 357 358 , , , 359 362 but but CC 363 369 little little JJ 370 372 is be VBZ 373 378 known know VBN 379 384 about about IN 385 394 signaling signaling NN 395 404 following follow VBG 405 409 CD40 cd40 NN 410 421 stimulation stimulation NN 422 424 of of IN 425 434 monocytes monocyte NNS 435 438 and and CC 439 446 resting rest VBG 447 456 tonsillar tonsillar JJ 457 458 B B NNP 459 464 cells cell NNS 464 465 . . . 466 475 Therefore therefore RB 475 476 , , , 477 479 we we PRP 480 487 studied study VBD 488 491 the the DT 492 496 CD40 cd40 NN 497 504 pathway pathway NN 505 507 in in IN 508 514 highly highly RB 515 523 purified purify VBN 524 529 human human JJ 530 539 monocytes monocyte NNS 540 543 and and CC 544 551 resting rest VBG 552 553 B B NNP 554 559 cells cell NNS 559 560 . . . 561 566 After after IN 567 571 CD40 CD40 NNP 572 582 triggering triggering NN 582 583 , , , 584 585 a a DT 586 593 similar similar JJ 594 604 activation activation NN 605 607 of of IN 608 611 the the DT 612 621 NF-kappaB NF-kappaB NNP 622 623 ( ( ( 623 626 but but CC 627 630 not not RB 631 633 of of IN 634 637 the the DT 638 642 AP-1 ap-1 NN 642 643 ) ) ) 644 657 transcription transcription NN 658 664 factor factor NN 665 672 complex complex NN 673 681 occurred occur VBD 682 684 in in IN 685 689 both both DT 690 694 cell cell NN 695 707 preparations preparation NNS 707 708 . . . 709 716 However however RB 716 717 , , , 718 721 the the DT 722 732 components component NNS 733 735 of of IN 736 739 the the DT 740 749 NF-kappaB NF-kappaB NNP 750 759 complexes complex NNS 760 764 were be VBD 765 774 different different JJ 775 777 in in IN 778 787 monocytes monocyte NNS 788 791 and and CC 792 793 B b NN 794 799 cells cell NNS 799 800 , , , 801 808 because because IN 809 812 p50 p50 NN 813 815 is be VBZ 816 820 part part NN 821 823 of of IN 824 827 the the DT 828 837 NF-kappaB NF-kappaB NNP 838 845 complex complex NN 846 853 induced induce VBN 854 856 by by IN 857 861 CD40 cd40 NN 862 872 triggering triggering NN 873 875 in in IN 876 880 both both CC 881 890 monocytes monocyte NNS 891 894 and and CC 895 896 B b NN 897 902 cells cell NNS 902 903 , , , 904 911 whereas whereas IN 912 915 p65 p65 NN 916 919 was be VBD 920 924 only only RB 925 932 induced induce VBN 933 935 in in IN 936 937 B B NNP 938 943 cells cell NNS 943 944 . . . 945 947 In in IN 948 956 contrast contrast NN 956 957 , , , 958 966 although although IN 967 970 the the DT 971 976 Janus Janus NNP 977 983 kinase kinase NNP 984 985 3 3 CD 986 994 tyrosine tyrosine NN 995 1001 kinase kinase NNP 1002 1005 was be VBD 1006 1016 associated associate VBN 1017 1021 with with IN 1022 1026 CD40 cd40 NN 1027 1036 molecules molecule NNS 1037 1039 in in IN 1040 1044 both both CC 1045 1054 monocytes monocyte NNS 1055 1058 and and CC 1059 1066 resting rest VBG 1067 1068 B B NNP 1069 1074 cells cell NNS 1074 1075 , , , 1076 1081 Janus Janus NNP 1082 1088 kinase kinase NNP 1089 1090 3 3 CD 1091 1106 phosphorylation phosphorylation NN 1107 1116 induction induction NN 1117 1120 was be VBD 1121 1129 observed observe VBN 1130 1134 only only RB 1135 1137 in in IN 1138 1152 CD40-activated cd40-activated JJ 1153 1162 monocytes monocyte NNS 1162 1163 , , , 1164 1168 with with IN 1169 1179 subsequent subsequent JJ 1180 1189 induction induction NN 1190 1192 of of IN 1193 1199 STAT5a STAT5a NNP 1200 1203 DNA DNA NNP 1204 1211 binding binding NN 1212 1220 activity activity NN 1221 1223 in in IN 1224 1227 the the DT 1228 1235 nucleus nucleus NN 1235 1236 . . . 1237 1242 These these DT 1243 1250 results result NNS 1251 1258 suggest suggest VBP 1259 1263 that that IN 1264 1267 the the DT 1268 1278 activation activation NN 1279 1286 signals signal NNS 1287 1289 in in IN 1290 1295 human human JJ 1296 1297 B B NNP 1298 1303 cells cell NNS 1304 1307 and and CC 1308 1317 monocytes monocyte NNS 1318 1324 differ differ VBP 1325 1334 following follow VBG 1335 1339 CD40 cd40 NN 1340 1351 stimulation stimulation NN 1351 1352 . . . 1353 1357 This this DT 1358 1369 observation observation NN 1370 1372 is be VBZ 1373 1383 consistent consistent JJ 1384 1388 with with IN 1389 1392 the the DT 1393 1402 detection detection NN 1403 1405 of of IN 1406 1412 normal normal JJ 1413 1425 CD40-induced cd40-induced JJ 1426 1434 monocyte monocyte NN 1435 1445 activation activation NN 1446 1448 in in IN 1449 1457 patients patient NNS 1458 1462 with with IN 1463 1467 CD40 CD40 NNP 1468 1475 ligand+ ligand+ JJ 1476 1481 hyper hyper NN 1482 1485 IgM IgM NNP 1486 1494 syndrome syndrome NN 1495 1497 in in IN 1498 1502 whom whom WP 1503 1504 a a DT 1505 1511 defect defect NN 1512 1514 in in IN 1515 1527 CD40-induced cd40-induced JJ 1528 1529 B B NNP 1530 1534 cell cell NN 1535 1545 activation activation NN 1546 1549 has have VBZ 1550 1554 been be VBN 1555 1563 reported report VBN 1563 1564 . . .