0 3 ETS ETS NNP 4 17 transcription transcription NN 18 25 factors factor NNS 26 34 regulate regulate VBP 35 37 an an DT 38 46 enhancer enhancer NN 47 55 activity activity NN 56 58 in in IN 59 62 the the DT 63 68 third third JJ 69 75 intron intron NN 76 78 of of IN 79 88 TNF-alpha TNF-alpha NNP 88 89 . . . 91 93 We we PRP 94 102 describe describe VBP 103 105 an an DT 106 114 enhancer enhancer NN 115 119 site site NN 120 122 in in IN 123 126 the the DT 127 132 third third JJ 133 139 intron intron NN 140 142 of of IN 143 148 tumor tumor NN 149 157 necrosis necrosis NN 158 164 factor factor NN 165 170 alpha alpha NN 171 172 ( ( ( 172 181 TNF-alpha TNF-alpha NNP 181 182 ) ) ) 182 183 . . . 184 185 A a DT 186 194 reporter reporter NN 195 204 construct construct NN 205 215 containing contain VBG 216 219 the the DT 220 231 5’-flanking 5'-flanking JJ 232 238 region region NN 239 241 of of IN 242 245 the the DT 246 251 mouse mouse NN 252 261 TNF-alpha TNF-alpha NNP 262 266 gene gene NN 267 276 displayed display VBD 277 281 weak weak JJ 282 290 activity activity NN 291 295 when when WRB 296 307 transfected transfecte VBN 308 312 into into IN 313 321 RAW264.7 raw264.7 NN 322 337 macrophage-like macrophage-like JJ 338 343 cells cell NNS 343 344 . . . 345 348 The the DT 349 357 addition addition NN 358 360 of of IN 361 364 the the DT 365 370 third third JJ 371 377 intron intron NN 378 380 of of IN 381 390 TNF-alpha TNF-alpha NNP 391 393 to to TO 394 398 this this DT 399 408 construct construct NN 409 417 resulted result VBD 418 420 in in IN 421 423 an an DT 424 435 enhancement enhancement NN 436 438 of of IN 439 442 CAT CAT NNP 443 450 protein protein NN 450 451 . . . 452 456 This this DT 457 468 enhancement enhancement NN 469 472 was be VBD 473 483 eliminated eliminate VBN 484 486 if if IN 487 488 a a DT 489 498 conserved conserve VBN 499 504 20-bp 20-bp JJ 505 513 sequence sequence NN 514 517 was be VBD 518 525 removed remove VBN 526 530 from from IN 531 534 the the DT 535 541 intron intron NN 542 544 or or CC 545 547 if if IN 548 549 a a DT 550 567 dominant-negative dominant-negative JJ 568 579 ets-binding ets-binding JJ 580 586 factor factor NN 587 590 was be VBD 591 605 co-transfected co-transfecte VBN 606 610 with with IN 611 614 the the DT 615 623 reporter reporter NN 624 628 gene gene NN 628 629 . . . 630 639 Mutations mutation NNS 640 642 of of IN 643 647 this this DT 648 652 site site NN 653 657 that that WDT 658 667 destroyed destroy VBD 668 677 potential potential JJ 678 681 ets ets NN 682 695 transcription transcription NN 696 702 factor factor NN 703 710 binding binding NN 711 716 sites site NNS 717 720 had have VBD 721 728 reduced reduce VBN 729 744 transcriptional transcriptional JJ 745 753 activity activity NN 753 754 . . . 755 758 The the DT 759 764 major major JJ 765 778 transcription transcription NN 779 785 factor factor NN 786 790 that that WDT 791 796 bound bind VBD 797 799 to to TO 800 803 the the DT 804 819 oligonucleotide oligonucleotide NN 820 823 was be VBD 824 833 confirmed confirm VBN 834 836 to to TO 837 839 be be VB 840 844 GABP GABP NNP 845 847 by by IN 848 858 supershift supershift NN 859 862 and and CC 863 874 competition competition NN 875 883 analysis analysis NN 883 884 . . . 885 887 In in IN 888 896 RAW264.7 raw264.7 NN 897 902 cells cell NNS 902 903 , , , 904 907 the the DT 908 915 binding binding NN 916 919 was be VBD 920 932 constitutive constitutive JJ 932 933 , , , 934 941 however however RB 941 942 , , , 943 945 in in IN 946 950 bone bone NN 951 965 marrow-derived marrow-derived JJ 966 977 macrophages macrophage NNS 978 985 binding binding NN 986 994 activity activity NN 995 998 was be VBD 999 1004 shown show VBN 1005 1007 to to TO 1008 1010 be be VB 1011 1027 interferon-gamma interferon-gamma NN 1028 1037 inducible inducible JJ 1037 1038 . . . 1039 1043 This this DT 1044 1047 may may MD 1048 1053 imply imply VB 1054 1055 a a DT 1056 1060 role role NN 1061 1064 for for IN 1065 1068 ets ets NN 1069 1082 transcription transcription NN 1083 1090 factors factor NNS 1091 1093 in in IN 1094 1097 the the DT 1098 1108 production production NN 1109 1111 of of IN 1112 1121 TNF-alpha TNF-alpha NNP 1121 1122 . . .