0 3 The the DT 4 8 role role NN 9 11 of of IN 12 15 jun jun NN 16 19 and and CC 20 23 fos fos NN 24 28 gene gene NN 29 35 family family NN 36 43 members member NNS 44 46 in in IN 47 83 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate NNP 84 91 induced induce VBD 92 103 hemopoietic hemopoietic JJ 104 119 differentiation differentiation NN 119 120 . . . 122 130 Terminal terminal JJ 131 146 differentiation differentiation NN 147 149 of of IN 150 153 the the DT 154 162 leukemic leukemic JJ 163 167 cell cell NN 168 173 lines line NNS 174 179 U-937 u-937 NN 180 183 and and CC 184 189 HL-60 HL-60 NNP 190 192 by by IN 193 229 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate NNP 230 232 is be VBZ 233 244 accompanied accompany VBN 245 247 by by IN 248 254 marked marked JJ 255 262 changes change NNS 263 265 in in IN 266 270 gene gene NN 271 281 expression expression NN 281 282 . . . 283 285 In in IN 286 290 this this DT 291 296 study study NN 296 297 , , , 298 300 we we PRP 301 312 demonstrate demonstrate VBP 313 317 that that IN 318 321 the the DT 322 332 expression expression NN 333 335 of of IN 336 339 jun jun NN 340 343 and and CC 344 347 fos fos NN 348 352 gene gene NN 353 359 family family NN 360 367 members member NNS 368 370 is be VBZ 371 378 induced induce VBN 379 383 with with IN 384 392 variable variable JJ 393 401 kinetics kinetic NNS 402 408 during during IN 409 445 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate NNP 446 453 induced induce VBD 454 469 differentiation differentiation NN 469 470 , , , 471 475 with with IN 476 481 c-jun c-jun NN 482 492 expression expression NN 493 497 best well RBS 498 509 paralleling paralleling JJ 510 525 differentiation differentiation NN 525 526 . . . 527 530 The the DT 531 541 generation generation NN 542 544 of of IN 545 549 AP-1 ap-1 NN 550 559 complexes complex NNS 559 560 , , , 561 563 as as IN 564 572 measured measure VBN 573 575 by by IN 576 579 DNA DNA NNP 580 587 binding binding NN 588 596 activity activity NN 596 597 , , , 598 605 closely closely RB 606 615 parallels parallel VBZ 616 629 morphological morphological JJ 630 645 differentiation differentiation NN 645 646 . . . 647 658 Furthermore furthermore RB 658 659 , , , 660 663 the the DT 664 671 ability ability NN 672 674 of of IN 675 680 these these DT 681 690 complexes complex NNS 691 693 to to TO 694 702 regulate regulate VB 703 707 gene gene NN 708 718 expression expression NN 719 721 is be VBZ 722 734 demonstrated demonstrate VBN 735 737 by by IN 738 747 increased increase VBN 748 761 transcription transcription NN 762 766 from from IN 767 769 an an DT 770 774 AP-1 ap-1 NN 775 781 driven driven JJ 782 790 reporter reporter NN 791 800 construct construct NN 801 804 and and CC 805 811 marked marked JJ 812 821 increases increase NNS 822 824 in in IN 825 828 the the DT 829 839 expression expression NN 840 842 of of IN 843 853 endogenous endogenous JJ 854 858 AP-1 ap-1 NN 859 868 regulated regulated JJ 869 874 genes gene NNS 874 875 . . . 876 891 Differentiation Differentiation NNP 892 898 assays assay NNS 899 904 using use VBG 905 910 water water NN 911 918 soluble soluble JJ 919 926 phorbol phorbol NN 927 933 esters ester NNS 934 940 reveal reveal VBP 941 945 that that IN 946 961 differentiation differentiation NN 962 969 becomes become VBZ 970 982 irreversible irreversible JJ 983 987 soon soon RB 988 993 after after IN 994 998 AP-1 ap-1 NN 999 1006 appears appear VBZ 1006 1007 . . . 1008 1012 This this DT 1013 1018 tight tight JJ 1019 1030 correlation correlation NN 1031 1038 between between IN 1039 1044 c-jun c-jun NN 1045 1055 expression expression NN 1055 1056 , , , 1057 1060 the the DT 1061 1071 generation generation NN 1072 1074 of of IN 1075 1079 AP-1 ap-1 NN 1080 1088 activity activity NN 1088 1089 , , , 1090 1093 and and CC 1094 1109 differentiation differentiation NN 1110 1118 suggests suggest VBZ 1119 1120 a a DT 1121 1129 critical critical JJ 1130 1134 role role NN 1135 1138 for for IN 1139 1143 this this DT 1144 1148 gene gene NN 1149 1152 and and CC 1153 1168 transcriptional transcriptional JJ 1169 1176 complex complex NN 1177 1183 during during IN 1184 1188 this this DT 1189 1196 process process NN 1196 1197 . . .