0 7 Nuclear nuclear JJ 8 14 factor factor NN 15 20 kappa kappa NN 21 22 B B NNP 23 32 activates activate VBZ 33 46 proenkephalin proenkephalin NN 47 60 transcription transcription NN 61 63 in in IN 64 65 T t NN 66 77 lymphocytes lymphocyte NNS 77 78 . . . 80 84 Upon upon IN 85 95 activation activation NN 95 96 , , , 97 98 T t NN 99 110 lymphocytes lymphocyte NNS 111 121 accumulate accumulate VBP 122 126 high high JJ 127 133 levels level NNS 134 136 of of IN 137 140 the the DT 141 153 neuropeptide neuropeptide NN 154 164 enkephalin enkephalin NN 165 170 which which WDT 171 180 correlate correlate VBP 181 185 with with IN 186 190 high high JJ 191 197 levels level NNS 198 200 of of IN 201 214 proenkephalin proenkephalin NN 215 219 mRNA mrna NN 220 222 in in IN 223 226 the the DT 227 232 cells cell NNS 232 233 . . . 234 238 Here here RB 239 241 we we PRP 242 254 investigated investigate VBD 255 258 the the DT 259 274 transcriptional transcriptional JJ 275 280 basis basis NN 281 284 for for IN 285 290 these these DT 291 298 changes change NNS 298 299 . . . 300 303 The the DT 304 317 proenkephalin proenkephalin NN 318 326 promoter promoter NN 327 335 contains contain VBZ 336 337 a a DT 338 346 sequence sequence NN 347 359 GGGGACGTCCCC GGGGACGTCCCC NNP 359 360 , , , 361 366 named name VBN 367 369 B2 b2 NN 369 370 , , , 371 376 which which WDT 377 379 is be VBZ 380 387 similar similar JJ 388 390 to to TO 391 394 the the DT 395 400 kappa kappa NN 401 402 B B NNP 403 411 sequence sequence NN 412 423 GGGGACTTTCC GGGGACTTTCC NNP 423 424 , , , 425 428 the the DT 429 436 binding bind VBG 437 441 site site NN 442 444 of of IN 445 448 the the DT 449 462 transcription transcription NN 463 469 factor factor NN 470 477 nuclear nuclear JJ 478 484 factor factor NN 485 486 ( ( ( 486 495 NF)-kappa NF)-kappa NNP 496 498 B. B. NNP 499 509 Activation Activation NNP 510 512 of of IN 513 514 T t NN 515 526 lymphocytes lymphocyte NNS 527 534 induces induce VBZ 535 537 an an DT 538 546 NF-kappa NF-kappa NNP 547 553 B-like b-like JJ 554 561 binding binding NN 562 570 activity activity NN 571 573 to to TO 574 577 the the DT 578 580 B2 b2 NN 581 585 site site NN 585 586 , , , 587 598 concomitant concomitant JJ 599 603 with with IN 604 614 activation activation NN 615 617 of of IN 618 621 the the DT 622 635 proenkephalin proenkephalin NN 636 644 promoter promoter NN 644 645 . . . 646 655 Mutations mutation NNS 656 658 at at IN 659 662 the the DT 663 665 B2 b2 NN 666 670 site site NN 671 678 abolish abolish VBP 679 683 this this DT 684 699 transcriptional transcriptional JJ 700 710 activation activation NN 710 711 . . . 712 715 The the DT 716 724 purified purify VBN 725 734 homodimer homodimer NN 735 736 ( ( ( 736 739 two two CD 740 744 p50s p50 NNS 744 745 ) ) ) 746 748 of of IN 749 752 the the DT 753 764 DNA-binding dna-binding JJ 765 772 subunit subunit NN 773 775 of of IN 776 784 NF-kappa NF-kappa NNP 785 786 B B NNP 787 792 binds bind VBZ 793 796 the the DT 797 799 B2 b2 NN 800 804 site site NN 805 807 of of IN 808 821 proenkephalin proenkephalin NN 822 832 relatively relatively RB 833 839 better well RBR 840 844 than than IN 845 849 does do VBZ 850 853 the the DT 854 868 heterotetramer heterotetramer NN 869 870 ( ( ( 870 873 two two CD 874 878 p65s p65 NNS 879 883 plus plus CC 884 887 two two CD 888 892 p50s p50 NNS 892 893 ) ) ) 894 898 form form NN 899 901 of of IN 902 905 the the DT 906 912 factor factor NN 912 913 . . . 914 918 Thus thus RB 918 919 , , , 920 922 it it PRP 923 930 appears appear VBZ 931 935 that that IN 936 939 the the DT 940 955 T-cell-specific t-cell-specific JJ 956 966 activation activation NN 967 969 of of IN 970 973 the the DT 974 987 proenkephalin proenkephalin NN 988 996 promoter promoter NN 997 999 is be VBZ 1000 1008 mediated mediate VBN 1009 1011 by by IN 1012 1020 NF-kappa NF-kappa NNP 1021 1023 B. B. NNP 1024 1031 However however RB 1031 1032 , , , 1033 1035 as as IN 1036 1044 NF-kappa NF-kappa NNP 1045 1046 B B NNP 1047 1049 is be VBZ 1050 1060 ubiquitous ubiquitous JJ 1061 1064 and and CC 1065 1068 the the DT 1069 1084 transcriptional transcriptional JJ 1085 1095 activation activation NN 1096 1103 through through IN 1104 1107 the the DT 1108 1110 B2 b2 NN 1111 1115 site site NN 1116 1118 is be VBZ 1119 1120 T t NN 1121 1125 cell cell NN 1126 1134 specific specific JJ 1134 1135 , , , 1136 1139 yet yet RB 1140 1147 another another DT 1148 1163 T-cell-specific t-cell-specific JJ 1164 1170 factor factor NN 1171 1176 which which WDT 1177 1187 synergizes synergize VBZ 1188 1192 with with IN 1193 1201 NF-kappa NF-kappa NNP 1202 1203 B B NNP 1204 1210 should should MD 1211 1213 be be VB 1214 1224 considered consider VBN 1224 1225 . . .