0 4 ETS1 ETS1 NNP 5 19 transactivates transactivate VBZ 20 23 the the DT 24 29 human human JJ 30 36 GM-CSF GM-CSF NNP 37 45 promoter promoter NN 46 48 in in IN 49 55 Jurkat Jurkat NNP 56 57 T t NN 58 63 cells cell NNS 64 74 stimulated stimulate VBN 75 79 with with IN 80 83 PMA PMA NNP 84 87 and and CC 88 97 ionomycin ionomycin NN 97 98 . . . 100 110 Activation Activation NNP 111 113 of of IN 114 115 T t NN 116 122 helper helper NN 123 128 cells cell NNS 129 136 results result VBZ 137 139 in in IN 140 150 coordinate coordinate JJ 151 161 expression expression NN 162 164 of of IN 165 166 a a DT 167 173 number number NN 174 176 of of IN 177 186 cytokines cytokine NNS 187 195 involved involve VBN 196 198 in in IN 199 214 differentiation differentiation NN 214 215 , , , 216 229 proliferation proliferation NN 230 233 and and CC 234 244 activation activation NN 245 247 of of IN 248 251 the the DT 252 266 haematopoietic haematopoietic JJ 267 273 system system NN 273 274 . . . 275 297 Granulocyte-macrophage granulocyte-macrophage JJ 298 316 colony-stimulating colony-stimulating JJ 317 323 factor factor NN 324 325 ( ( ( 325 331 GM-CSF GM-CSF NNP 331 332 ) ) ) 333 335 is be VBZ 336 339 one one CD 340 344 such such JJ 345 353 cytokine cytokine NN 354 359 whose whose WP$ 360 369 increased increase VBN 370 380 expression expression NN 381 388 results result VBZ 389 395 partly partly RB 396 400 from from IN 401 410 increases increase NNS 411 413 in in IN 414 427 transcription transcription NN 427 428 . . . 429 439 Cis-acting cis-acting JJ 440 448 elements element NNS 449 453 with with IN 454 456 NF NF NNP 457 462 kappa kappa NN 463 464 B B NNP 464 465 , , , 466 470 AP-1 ap-1 NN 471 474 and and CC 475 483 ETS-like ets-like JJ 484 490 motifs motif NNS 491 495 have have VBP 496 500 been be VBN 501 511 identified identify VBN 512 514 in in IN 515 518 the the DT 519 527 promoter promoter NN 528 534 region region NN 535 537 of of IN 538 541 the the DT 542 548 GM-CSF GM-CSF NNP 549 553 gene gene NN 553 554 , , , 555 560 which which WDT 561 564 are be VBP 565 574 important important JJ 575 578 for for IN 579 594 transcriptional transcriptional JJ 595 603 activity activity NN 604 613 following follow VBG 614 617 PMA PMA NNP 618 621 and and CC 622 631 ionomycin ionomycin NN 632 643 stimulation stimulation NN 643 644 . . . 645 646 A a DT 647 653 number number NN 654 656 of of IN 657 660 the the DT 661 664 ETS ETS NNP 665 671 family family NN 672 674 of of IN 675 688 transcription transcription NN 689 696 factors factor NNS 697 700 are be VBP 701 710 expressed express VBN 711 713 in in IN 714 715 T t NN 716 721 cells cell NNS 721 722 , , , 723 732 including include VBG 733 737 ETS1 ETS1 NNP 738 741 and and CC 742 746 ELF1 ELF1 NNP 746 747 . . . 748 752 Here here RB 753 755 we we PRP 756 764 describe describe VBP 765 768 the the DT 769 776 ability ability NN 777 779 of of IN 780 785 these these DT 786 793 factors factor NNS 794 796 to to TO 797 805 interact interact VB 806 810 with with IN 811 812 a a DT 813 817 site site NN 818 819 ( ( ( 819 822 GM5 GM5 NNP 822 823 ) ) ) 823 824 , , , 825 832 located located JJ 833 839 within within IN 840 843 the the DT 844 848 CLE0 cle0 NN 849 856 element element NN 856 857 , , , 858 861 -47 -47 CD 862 864 to to TO 865 868 -40 -40 CD 869 877 upstream upstream RB 878 880 of of IN 881 884 the the DT 885 891 GM-CSF GM-CSF NNP 892 905 transcription transcription NN 906 916 initiation initiation NN 917 921 site site NN 921 922 . . . 923 932 Exogenous exogenous JJ 933 937 ETS1 ETS1 NNP 937 938 , , , 939 942 but but CC 943 946 not not RB 947 951 ELF1 elf1 NN 951 952 , , , 953 956 can can MD 957 970 transactivate transactivate VB 971 977 GM-CSF GM-CSF NNP 977 978 , , , 979 986 through through IN 987 990 the the DT 991 994 GM5 GM5 NNP 995 999 site site NN 999 1000 , , , 1001 1003 in in IN 1004 1005 a a DT 1006 1019 PMA/ionomycin PMA/ionomycin NNP 1020 1029 dependent dependent JJ 1030 1036 manner manner NN 1036 1037 . . . 1038 1043 Other other JJ 1044 1056 unidentified unidentified JJ 1057 1065 ETS-like ets-like JJ 1066 1073 factors factor NNS 1074 1081 present present JJ 1082 1084 in in IN 1085 1091 Jurkat Jurkat NNP 1092 1097 cells cell NNS 1098 1101 are be VBP 1102 1106 also also RB 1107 1114 capable capable JJ 1115 1117 of of IN 1118 1125 binding bind VBG 1126 1129 GM5 gm5 NN 1129 1130 . . . 1131 1139 Mutation mutation NN 1140 1142 of of IN 1143 1146 the the DT 1147 1151 core core NN 1152 1155 ETS ets NN 1156 1163 binding binding NN 1164 1168 site site NN 1169 1173 from from IN 1174 1180 -GGAA- -ggaa- NN 1181 1183 to to TO 1184 1190 -GGAT- -GGAT- NNP 1191 1199 prevents prevent VBZ 1200 1203 the the DT 1204 1211 binding binding NN 1212 1214 of of IN 1215 1223 ETS-like ets-like JJ 1224 1231 factors factor NNS 1232 1236 with with IN 1237 1240 the the DT 1241 1250 exception exception NN 1251 1253 of of IN 1254 1258 ETS1 ETS1 NNP 1258 1259 . . . 1260 1263 The the DT 1264 1270 GM-CSF GM-CSF NNP 1271 1279 promoter promoter NN 1279 1280 , , , 1281 1289 modified modify VBN 1290 1292 in in IN 1293 1297 this this DT 1298 1301 way way NN 1302 1304 to to TO 1305 1307 be be VB 1308 1312 ETS1 ets1 NN 1313 1321 specific specific JJ 1321 1322 , , , 1323 1325 is be VBZ 1326 1331 fully fully RB 1332 1342 responsive responsive JJ 1343 1345 to to TO 1346 1359 PMA/ionomycin PMA/ionomycin NNP 1360 1369 induction induction NN 1369 1370 , , , 1371 1373 in in IN 1374 1382 addition addition NN 1383 1385 to to TO 1386 1390 ETS1 ets1 NN 1391 1406 transactivation transactivation NN 1407 1409 in in IN 1410 1413 the the DT 1414 1422 presence presence NN 1423 1425 of of IN 1426 1429 PMA PMA NNP 1430 1433 and and CC 1434 1443 ionomycin ionomycin NN 1443 1444 . . . 1445 1453 Together together RB 1454 1459 these these DT 1460 1464 data datum NNS 1465 1472 suggest suggest VBP 1473 1477 that that IN 1478 1482 ETS1 ets1 NN 1483 1486 may may MD 1487 1489 be be VB 1490 1498 involved involve VBN 1499 1501 in in IN 1502 1511 mediating mediate VBG 1512 1515 the the DT 1516 1525 increased increase VBN 1526 1532 GM-CSF GM-CSF NNP 1533 1543 production production NN 1544 1554 associated associate VBN 1555 1559 with with IN 1560 1561 T t NN 1562 1566 cell cell NN 1567 1577 activation activation NN 1577 1578 . . .