0 15 Tissue-specific tissue-specific JJ 16 26 regulation regulation NN 27 29 of of IN 30 33 the the DT 34 40 rabbit rabbit NN 41 56 15-lipoxygenase 15-lipoxygenase NN 57 61 gene gene NN 62 64 in in IN 65 74 erythroid erythroid JJ 75 80 cells cell NNS 81 83 by by IN 84 85 a a DT 86 101 transcriptional transcriptional JJ 102 110 silencer silencer NN 110 111 . . . 113 116 The the DT 117 132 15-lipoxygenase 15-lipoxygenase NN 133 134 ( ( ( 134 137 lox lox NN 137 138 ) ) ) 139 143 gene gene NN 144 146 is be VBZ 147 156 expressed express VBN 157 159 in in IN 160 161 a a DT 162 177 tissue-specific tissue-specific JJ 178 184 manner manner NN 184 185 , , , 186 199 predominantly predominantly RB 200 202 in in IN 203 212 erythroid erythroid JJ 213 218 cells cell NNS 219 222 but but CC 223 227 also also RB 228 230 in in IN 231 237 airway airway NN 238 248 epithelial epithelial JJ 249 254 cells cell NNS 255 258 and and CC 259 270 eosinophils eosinophil NNS 270 271 . . . 272 274 We we PRP 275 286 demonstrate demonstrate VBP 287 289 in in IN 290 294 this this DT 295 301 report report NN 302 306 that that IN 307 310 the the DT 311 312 5 5 CD 312 313 ’ ' SYM 314 322 flanking flank VBG 323 326 DNA dna NN 327 329 of of IN 330 333 the the DT 334 340 15-lox 15-lox JJ 341 345 gene gene NN 346 354 contains contain VBZ 355 364 sequences sequence NNS 365 370 which which WDT 371 384 down-regulate down-regulate VBP 385 388 its its PRP$ 389 397 activity activity NN 398 400 in in IN 401 402 a a DT 403 410 variety variety NN 411 413 of of IN 414 427 non-erythroid non-erythroid JJ 428 432 cell cell NN 433 438 lines line NNS 439 442 but but CC 443 446 not not RB 447 449 in in IN 450 453 two two CD 454 463 erythroid erythroid JJ 464 468 cell cell NN 469 474 lines line NNS 474 475 . . . 476 479 The the DT 480 487 element element NN 488 491 has have VBZ 492 507 characteristics characteristic NNS 508 510 of of IN 511 512 a a DT 513 528 transcriptional transcriptional JJ 529 530 ‘ ` `` 530 538 silencer silencer NN 538 539 ’ ' '' 540 545 since since IN 546 548 it it PRP 549 558 functions function VBZ 559 561 in in IN 562 566 both both DT 567 579 orientations orientation NNS 579 580 . . . 581 584 The the DT 585 589 main main JJ 590 598 activity activity NN 599 601 of of IN 602 605 the the DT 606 614 silencer silencer NN 615 618 has have VBZ 619 623 been be VBN 624 630 mapped map VBN 631 633 to to TO 634 637 the the DT 638 643 first first JJ 644 647 900 900 CD 648 650 bp bp NN 651 653 of of IN 654 655 5 5 CD 655 656 ’ ' SYM 657 665 flanking flanking NN 666 669 DNA dna NN 669 670 , , , 671 676 which which WDT 677 685 contains contain VBZ 686 690 nine nine CD 691 698 binding bind VBG 699 704 sites site NNS 705 708 for for IN 709 710 a a DT 711 718 nuclear nuclear JJ 719 725 factor factor NN 726 733 present present JJ 734 736 in in IN 737 750 non-erythroid non-erythroid JJ 751 756 cells cell NNS 757 760 but but CC 761 764 not not RB 765 767 in in IN 768 777 erythroid erythroid JJ 778 783 cells cell NNS 783 784 . . . 785 790 These these DT 791 798 binding bind VBG 799 804 sites site NNS 805 809 have have VBP 810 817 similar similar JJ 818 827 sequences sequence NNS 828 831 and and CC 832 840 multiple multiple JJ 841 847 copies copy NNS 848 850 of of IN 851 854 the the DT 855 862 binding bind VBG 863 868 sites site NNS 869 875 confer confer VBP 876 891 tissue-specific tissue-specific JJ 892 907 down-regulation down-regulation NN 908 912 when when WRB 913 921 attached attach VBN 922 924 to to TO 925 926 a a DT 927 934 minimal minimal JJ 935 938 lox lox NN 939 947 promoter promoter NN 948 956 fragment fragment NN 956 957 . . . 958 961 The the DT 962 963 5 5 CD 963 964 ’ ' SYM 965 973 flanking flank VBG 974 977 DNA dna NN 978 982 also also RB 983 991 contains contain VBZ 992 993 a a DT 994 1001 cluster cluster NN 1002 1004 of of IN 1005 1010 three three CD 1011 1018 binding bind VBG 1019 1024 sites site NNS 1025 1028 for for IN 1029 1032 the the DT 1033 1037 GATA GATA NNP 1038 1044 family family NN 1045 1047 of of IN 1048 1061 transcription transcription NN 1062 1069 factors factor NNS 1069 1070 . . .