0	13	Costimulation	costimulation	NN
14	25	requirement	requirement	NN
26	29	for	for	IN
30	34	AP-1	AP-1	NNP
35	38	and	and	CC
39	47	NF-kappa	NF-kappa	NNP
48	49	B	B	NNP
50	63	transcription	transcription	NN
64	70	factor	factor	NN
71	81	activation	activation	NN
82	84	in	in	IN
85	86	T	t	NN
87	92	cells	cell	NNS
92	93	.	.	.

95	98	The	the	DT
99	114	transcriptional	transcriptional	JJ
115	123	activity	activity	NN
124	126	of	of	IN
127	130	the	the	DT
131	135	IL-2	il-2	NN
136	144	promoter	promoter	NN
145	153	requires	require	VBZ
154	160	T-cell	t-cell	NN
161	174	costimulation	costimulation	NN
175	184	delivered	deliver	VBN
185	187	by	by	IN
188	191	the	the	DT
192	195	TCR	TCR	NNP
196	199	and	and	CC
200	203	the	the	DT
204	213	auxiliary	auxiliary	JJ
214	222	receptor	receptor	NN
223	227	CD28	cd28	NN
227	228	.	.	.

229	236	Several	several	JJ
237	250	transcription	transcription	NN
251	258	factors	factor	NNS
259	270	participate	participate	VBP
271	273	in	in	IN
274	278	IL-2	il-2	NN
279	287	promoter	promoter	NN
288	298	activation	activation	NN
298	299	,	,	,
300	305	among	among	IN
306	311	which	which	WDT
312	315	are	be	VBP
316	325	AP-1-like	ap-1-like	JJ
326	333	factors	factor	NNS
334	337	and	and	CC
338	346	NF-kappa	NF-kappa	NNP
347	349	B.	B.	NNP
350	357	Protein	Protein	NNP
358	373	phosphorylation	phosphorylation	NN
374	377	has	have	VBZ
378	380	an	an	DT
381	390	important	important	JJ
391	395	role	role	NN
396	398	in	in	IN
399	402	the	the	DT
403	413	regulation	regulation	NN
414	416	of	of	IN
417	422	these	these	DT
423	426	two	two	CD
427	434	factors	factor	NNS
434	435	:	:	:
436	437	(	(	(
437	438	1	1	LS
438	439	)	)	)
440	442	it	it	PRP
443	450	induces	induce	VBZ
451	454	the	the	DT
455	470	transactivating	transactivate	VBG
471	479	capacity	capacity	NN
480	482	of	of	IN
483	486	the	the	DT
487	491	AP-1	ap-1	NN
492	499	protein	protein	NN
500	505	c-Jun	c-jun	NN
505	506	;	;	:
507	510	and	and	CC
511	512	(	(	(
512	513	2	2	LS
513	514	)	)	)
515	517	it	it	PRP
518	520	is	be	VBZ
521	529	involved	involve	VBN
530	532	in	in	IN
533	536	the	the	DT
537	544	release	release	NN
545	547	of	of	IN
548	551	the	the	DT
552	563	cytoplasmic	cytoplasmic	JJ
564	573	inhibitor	inhibitor	NN
573	574	,	,	,
575	576	I	i	NN
577	582	kappa	kappa	NN
583	584	B	B	NNP
584	585	,	,	,
586	590	from	from	IN
591	599	NF-kappa	NF-kappa	NNP
600	601	B	B	NNP
601	602	,	,	,
603	611	allowing	allow	VBG
612	625	translocation	translocation	NN
626	628	of	of	IN
629	632	the	the	DT
633	639	latter	latter	JJ
640	644	into	into	IN
645	648	the	the	DT
649	656	nucleus	nucleus	NN
656	657	.	.	.

658	660	We	we	PRP
661	665	have	have	VBP
666	674	recently	recently	RB
675	680	shown	show	VBN
681	685	that	that	IN
686	690	both	both	CC
691	706	phosphorylation	phosphorylation	NN
707	716	processes	process	NNS
717	724	require	require	VBP
725	731	T-cell	t-cell	NN
732	745	costimulation	costimulation	NN
745	746	.	.	.

747	758	Furthermore	furthermore	RB
758	759	,	,	,
760	762	in	in	IN
763	772	activated	activate	VBN
773	774	T	t	NN
775	780	cells	cell	NNS
780	781	,	,	,
782	785	the	the	DT
786	794	kinetics	kinetic	NNS
795	797	of	of	IN
798	801	the	the	DT
802	805	two	two	CD
806	821	phosphorylation	phosphorylation	NN
822	828	events	event	NNS
829	832	are	be	VBP
833	844	essentially	essentially	RB
845	852	similar	similar	JJ
852	853	.	.	.

854	863	According	accord	VBG
864	866	to	to	TO
867	870	our	our	PRP$
871	878	results	result	NNS
878	879	,	,	,
880	887	however	however	RB
887	888	,	,	,
889	892	the	the	DT
893	900	kinases	kinase	NNS
901	912	responsible	responsible	JJ
913	916	for	for	IN
917	920	the	the	DT
921	924	two	two	CD
925	934	processes	process	NNS
935	938	are	be	VBP
939	947	distinct	distinct	JJ
948	956	entities	entity	NNS
956	957	.	.	.

958	965	Whereas	whereas	IN
966	970	TPCK	TPCK	NNP
971	979	inhibits	inhibit	VBZ
980	995	phosphorylation	phosphorylation	NN
996	998	of	of	IN
999	1000	I	i	NN
1001	1006	kappa	kappa	NN
1007	1008	B	B	NNP
1009	1012	and	and	CC
1012	1013	,	,	,
1014	1026	consequently	consequently	RB
1026	1027	,	,	,
1028	1038	activation	activation	NN
1039	1041	of	of	IN
1042	1050	NF-kappa	NF-kappa	NNP
1051	1052	B	B	NNP
1052	1053	,	,	,
1054	1056	it	it	PRP
1057	1065	markedly	markedly	RB
1066	1074	enhances	enhance	VBZ
1075	1078	the	the	DT
1079	1087	activity	activity	NN
1088	1090	of	of	IN
1091	1094	JNK	JNK	NNP
1094	1095	,	,	,
1096	1099	the	the	DT
1100	1103	MAP	MAP	NNP
1104	1118	kinase-related	kinase-related	JJ
1119	1125	kinase	kinase	NNP
1126	1130	that	that	WDT
1131	1145	phosphorylates	phosphorylate	VBZ
1146	1149	the	the	DT
1150	1165	transactivation	transactivation	NN
1166	1172	domain	domain	NN
1173	1175	of	of	IN
1176	1181	c-Jun	c-jun	NN
1181	1182	.	.	.

1183	1185	We	we	PRP
1185	1186	,	,	,
1187	1196	therefore	therefore	RB
1196	1197	,	,	,
1198	1205	propose	propose	VBP
1206	1209	the	the	DT
1210	1220	activation	activation	NN
1221	1227	scheme	scheme	NN
1228	1237	presented	present	VBN
1238	1240	in	in	IN
1241	1247	FIGURE	FIGURE	NNP
1248	1249	3	3	CD
1250	1253	for	for	IN
1254	1260	T-cell	t-cell	NN
1261	1274	costimulation	costimulation	NN
1274	1275	.	.	.

1276	1289	Costimulation	costimulation	NN
1290	1297	results	result	VBZ
1298	1300	in	in	IN
1301	1304	the	the	DT
1305	1315	activation	activation	NN
1316	1318	of	of	IN
1319	1320	a	a	DT
1321	1330	signaling	signaling	NN
1331	1338	pathway	pathway	NN
1339	1343	that	that	WDT
1344	1349	leads	lead	VBZ
1350	1352	to	to	TO
1353	1356	the	the	DT
1357	1369	simultaneous	simultaneous	JJ
1370	1379	induction	induction	NN
1380	1382	of	of	IN
1383	1386	the	the	DT
1387	1390	two	two	CD
1391	1404	transcription	transcription	NN
1405	1412	factors	factor	NNS
1412	1413	,	,	,
1414	1418	AP-1	AP-1	NNP
1419	1422	and	and	CC
1423	1431	NF-kappa	NF-kappa	NNP
1432	1434	B.	B.	NNP
1435	1446	Integration	Integration	NNP
1447	1449	of	of	IN
1450	1453	the	the	DT
1454	1461	signals	signal	NNS
1462	1471	generated	generate	VBN
1472	1474	by	by	IN
1475	1478	TCR	TCR	NNP
1479	1482	and	and	CC
1483	1487	CD28	cd28	NN
1488	1498	engagement	engagement	NN
1499	1505	occurs	occur	VBZ
1506	1511	along	along	IN
1512	1516	this	this	DT
1517	1524	pathway	pathway	NN
1524	1525	,	,	,
1526	1531	which	which	WDT
1532	1536	then	then	RB
1537	1547	bifurcates	bifurcate	VBZ
1548	1550	to	to	TO
1551	1557	induce	induce	VB
1558	1559	I	i	NN
1560	1565	kappa	kappa	NN
1566	1567	B	B	NNP
1568	1583	phosphorylation	phosphorylation	NN
1584	1587	and	and	CC
1588	1596	NF-kappa	NF-kappa	NNP
1597	1598	B	B	NNP
1599	1609	activation	activation	NN
1610	1612	on	on	IN
1613	1616	the	the	DT
1617	1620	one	one	CD
1621	1625	hand	hand	NN
1625	1626	,	,	,
1627	1630	and	and	CC
1631	1634	JNK	JNK	NNP
1635	1645	activation	activation	NN
1646	1649	and	and	CC
1650	1655	c-Jun	c-jun	NN
1656	1671	phosphorylation	phosphorylation	NN
1672	1674	on	on	IN
1675	1678	the	the	DT
1679	1684	other	other	JJ
1684	1685	.	.	.

1686	1688	We	we	PRP
1689	1692	are	be	VBP
1693	1702	currently	currently	RB
1703	1710	engaged	engage	VBN
1711	1713	in	in	IN
1714	1722	defining	define	VBG
1723	1728	where	where	WRB
1729	1732	the	the	DT
1733	1736	two	two	CD
1737	1744	signals	signal	NNS
1745	1754	integrate	integrate	VBP
1755	1760	along	along	IN
1761	1764	the	the	DT
1765	1778	AP-1/NF-kappa	AP-1/NF-kappa	NNP
1779	1780	B	B	NNP
1781	1788	pathway	pathway	NN
1788	1789	.	.	.