0 14 Identification identification NN 15 17 of of IN 18 20 an an DT 21 22 I i NN 23 28 kappa kappa NN 29 30 B B NNP 31 47 alpha-associated alpha-associated JJ 48 55 protein protein NN 56 62 kinase kinase NNP 63 65 in in IN 66 67 a a DT 68 73 human human JJ 74 83 monocytic monocytic JJ 84 88 cell cell NN 89 93 line line NN 94 97 and and CC 98 111 determination determination NN 112 114 of of IN 115 118 its its PRP$ 119 134 phosphorylation phosphorylation NN 135 140 sites site NNS 141 143 on on IN 144 145 I i NN 146 151 kappa kappa NN 152 153 B B NNP 154 159 alpha alpha NN 159 160 . . . 162 169 Nuclear nuclear JJ 170 176 factor factor NN 177 182 kappa kappa NN 183 184 B B NNP 185 186 ( ( ( 186 194 NF-kappa NF-kappa NNP 195 196 B B NNP 196 197 ) ) ) 198 200 is be VBZ 201 207 stored store VBN 208 210 in in IN 211 214 the the DT 215 224 cytoplasm cytoplasm NN 225 227 as as IN 228 230 an an DT 231 239 inactive inactive JJ 240 244 form form NN 245 252 through through IN 253 264 interaction interaction NN 265 269 with with IN 270 271 I i NN 272 277 kappa kappa NN 278 280 B. B. NNP 281 292 Stimulation Stimulation NNP 293 295 of of IN 296 301 cells cell NNS 302 307 leads lead VBZ 308 310 to to TO 311 312 a a DT 313 318 rapid rapid JJ 319 334 phosphorylation phosphorylation NN 335 337 of of IN 338 339 I i NN 340 345 kappa kappa NN 346 347 B B NNP 348 353 alpha alpha NN 353 354 , , , 355 360 which which WDT 361 363 is be VBZ 364 372 presumed presume VBN 373 375 to to TO 376 378 be be VB 379 388 important important JJ 389 392 for for IN 393 396 the the DT 397 407 subsequent subsequent JJ 408 419 degradation degradation NN 419 420 . . . 421 423 We we PRP 424 428 have have VBP 429 437 recently recently RB 438 446 reported report VBN 447 450 the the DT 451 464 establishment establishment NN 465 467 of of IN 468 469 a a DT 470 488 lipopolysaccharide lipopolysaccharide NN 489 490 ( ( ( 490 504 LPS)-dependent lps)-dependent JJ 505 514 cell-free cell-free JJ 515 525 activation activation NN 526 532 system system NN 533 535 of of IN 536 544 NF-kappa NF-kappa NNP 545 546 B B NNP 547 549 in in IN 550 561 association association NN 562 566 with with IN 567 570 the the DT 571 580 induction induction NN 581 583 of of IN 584 585 I i NN 586 591 kappa kappa NN 592 593 B B NNP 594 599 alpha alpha NN 600 615 phosphorylation phosphorylation NN 615 616 . . . 617 619 In in IN 620 624 this this DT 625 630 study study NN 630 631 , , , 632 634 we we PRP 635 639 have have VBP 640 650 identified identify VBN 651 652 a a DT 653 659 kinase kinase NNP 660 662 in in IN 663 667 cell cell NN 668 676 extracts extract NNS 677 681 from from IN 682 685 the the DT 686 700 LPS-stimulated lps-stimulated JJ 701 706 human human JJ 707 716 monocytic monocytic JJ 717 721 cell cell NN 722 726 line line NN 726 727 , , , 728 733 THP-1 thp-1 NN 733 734 , , , 735 739 that that WDT 740 752 specifically specifically RB 753 758 binds bind VBZ 759 762 and and CC 763 777 phosphorylates phosphorylate VBZ 778 779 I i NN 780 785 kappa kappa NN 786 787 B B NNP 788 793 alpha alpha NN 793 794 . . . 795 798 LPS LPS NNP 799 810 stimulation stimulation NN 811 822 transiently transiently RB 823 831 enhanced enhance VBD 832 835 the the DT 836 837 I i NN 838 843 kappa kappa NN 844 845 B B NNP 846 857 alpha-bound alpha-bound JJ 858 864 kinase kinase NNP 865 873 activity activity NN 874 876 in in IN 877 882 THP-1 thp-1 NN 883 888 cells cell NNS 888 889 . . . 890 900 Mutational mutational JJ 901 909 analyses analysis NNS 910 912 of of IN 913 914 I i NN 915 920 kappa kappa NN 921 922 B B NNP 923 928 alpha alpha NN 929 932 and and CC 933 944 competition competition NN 945 956 experiments experiment NNS 957 961 with with IN 962 965 the the DT 966 975 synthetic synthetic JJ 976 984 peptides peptide NNS 985 995 identified identify VBD 996 1001 major major JJ 1002 1017 phosphorylation phosphorylation NN 1018 1023 sites site NNS 1024 1026 by by IN 1027 1030 the the DT 1031 1036 bound bind VBN 1037 1043 kinase kinase NNP 1044 1046 as as IN 1047 1050 Ser Ser NNP 1051 1054 and and CC 1055 1058 Thr Thr NNP 1059 1067 residues residue NNS 1068 1070 in in IN 1071 1074 the the DT 1075 1085 C-terminal c-terminal JJ 1086 1092 acidic acidic JJ 1093 1099 domain domain NN 1100 1102 of of IN 1103 1104 I i NN 1105 1110 kappa kappa NN 1111 1112 B B NNP 1113 1118 alpha alpha NN 1118 1119 . . . 1120 1128 Moreover moreover RB 1128 1129 , , , 1130 1132 we we PRP 1133 1137 show show VBP 1138 1142 that that IN 1143 1146 the the DT 1147 1154 peptide peptide NN 1154 1155 , , , 1156 1169 corresponding correspond VBG 1170 1172 to to TO 1173 1176 the the DT 1177 1187 C-terminal c-terminal JJ 1188 1194 acidic acidic JJ 1195 1201 domain domain NN 1202 1204 of of IN 1205 1206 I i NN 1207 1212 kappa kappa NN 1213 1214 B B NNP 1215 1220 alpha alpha NN 1220 1221 , , , 1222 1229 blocked block VBD 1230 1233 the the DT 1234 1245 LPS-induced lps-induced JJ 1246 1254 NF-kappa NF-kappa NNP 1255 1256 B B NNP 1257 1267 activation activation NN 1268 1270 as as RB 1271 1275 well well RB 1276 1278 as as IN 1279 1288 inducible inducible JJ 1289 1304 phosphorylation phosphorylation NN 1305 1307 of of IN 1308 1318 endogenous endogenous JJ 1319 1320 I i NN 1321 1326 kappa kappa NN 1327 1328 B B NNP 1329 1334 alpha alpha NN 1335 1337 in in IN 1338 1339 a a DT 1340 1349 cell-free cell-free JJ 1350 1356 system system NN 1357 1362 using use VBG 1363 1368 THP-1 thp-1 NN 1369 1374 cells cell NNS 1374 1375 . . . 1376 1381 These these DT 1382 1389 results result NNS 1390 1399 suggested suggest VBD 1400 1404 that that IN 1405 1408 the the DT 1409 1414 bound bind VBN 1415 1421 kinase kinase NNP 1422 1424 is be VBZ 1425 1433 involved involve VBN 1434 1436 in in IN 1437 1440 the the DT 1441 1450 signaling signaling NN 1451 1458 pathway pathway NN 1459 1461 of of IN 1462 1465 LPS lps NN 1466 1468 by by IN 1469 1477 inducing induce VBG 1478 1481 the the DT 1482 1497 phosphorylation phosphorylation NN 1498 1500 of of IN 1501 1504 the the DT 1505 1515 C-terminal c-terminal JJ 1516 1522 region region NN 1523 1525 of of IN 1526 1527 I i NN 1528 1533 kappa kappa NN 1534 1535 B B NNP 1536 1541 alpha alpha NN 1542 1545 and and CC 1546 1556 subsequent subsequent JJ 1557 1569 dissociation dissociation NN 1570 1572 of of IN 1573 1576 the the DT 1577 1585 NF-kappa NF-kappa NNP 1586 1589 B.I B.I NNP 1590 1595 kappa kappa NN 1596 1597 B B NNP 1598 1603 alpha alpha NN 1604 1611 complex complex NN 1611 1612 . . .