0 8 Monocyte Monocyte NNP 9 18 tethering tethering NN 19 21 by by IN 22 32 P-selectin P-selectin NNP 33 42 regulates regulate VBZ 43 51 monocyte monocyte NN 52 63 chemotactic chemotactic JJ 64 73 protein-1 protein-1 NN 74 77 and and CC 78 83 tumor tumor NN 84 92 necrosis necrosis NN 93 105 factor-alpha factor-alpha NN 106 115 secretion secretion NN 115 116 . . . 117 123 Signal Signal NNP 124 135 integration integration NN 136 139 and and CC 140 148 NF-kappa NF-kappa NNP 149 150 B B NNP 151 164 translocation translocation NN 165 166 [ [ ( 166 169 see see VB 170 178 comments comment NNS 178 179 ] ] ) 181 189 Adhesion Adhesion NNP 190 199 molecules molecule NNS 200 204 that that WDT 205 211 tether tether VBP 212 223 circulating circulate VBG 224 234 leukocytes leukocyte NNS 235 237 to to TO 238 249 endothelial endothelial JJ 250 255 cells cell NNS 256 259 may may MD 260 264 also also RB 265 274 transduce transduce VB 275 277 or or CC 278 286 modulate modulate VB 287 297 outside-in outside-in JJ 298 305 signals signal NNS 306 309 for for IN 310 318 cellular cellular JJ 319 329 activation activation NN 329 330 , , , 331 340 providing provide VBG 341 343 an an DT 344 351 initial initial JJ 352 362 regulatory regulatory JJ 363 368 point point NN 369 371 in in IN 372 375 the the DT 376 388 inflammatory inflammatory JJ 389 397 response response NN 397 398 . . . 399 407 Adhesion Adhesion NNP 408 410 of of IN 411 416 human human JJ 417 426 monocytes monocyte NNS 427 429 to to TO 430 440 P-selectin P-selectin NNP 440 441 , , , 442 445 the the DT 446 450 most most RBS 451 458 rapidly rapidly RB 459 468 expressed express VBN 469 480 endothelial endothelial JJ 481 490 tethering tethering NN 491 497 factor factor NN 497 498 , , , 499 508 increased increase VBD 509 512 the the DT 513 522 secretion secretion NN 523 525 of of IN 526 534 monocyte monocyte NN 535 546 chemotactic chemotactic JJ 547 556 protein-1 protein-1 NN 557 558 ( ( ( 558 563 MCP-1 mcp-1 NN 563 564 ) ) ) 565 568 and and CC 569 574 tumor tumor NN 575 583 necrosis necrosis NN 584 596 factor-alpha factor-alpha NN 597 598 ( ( ( 598 607 TNF-alpha TNF-alpha NNP 607 608 ) ) ) 609 611 by by IN 612 615 the the DT 616 626 leukocytes leukocyte NNS 627 631 when when WRB 632 636 they they PRP 637 641 were be VBD 642 652 stimulated stimulate VBN 653 657 with with IN 658 677 platelet-activating platelet-activating JJ 678 684 factor factor NN 684 685 . . . 686 695 Increased increase VBN 696 704 cytokine cytokine NN 705 714 secretion secretion NN 715 718 was be VBD 719 731 specifically specifically RB 732 741 inhibited inhibit VBN 742 744 by by IN 745 747 G1 G1 NNP 747 748 , , , 749 751 an an DT 752 767 anti-P-selectin anti-p-selectin JJ 768 771 mAb mab NN 772 776 that that WDT 777 785 prevents prevent VBZ 786 796 P-selectin P-selectin NNP 797 801 from from IN 802 809 binding bind VBG 810 812 to to TO 813 816 its its PRP$ 817 823 ligand ligand NN 824 825 ( ( ( 825 835 P-selectin P-selectin NNP 836 848 glycoprotein glycoprotein NN 849 857 ligand-1 ligand-1 NN 857 858 ) ) ) 859 861 on on IN 862 869 myeloid myeloid JJ 870 875 cells cell NNS 875 876 . . . 877 885 Moreover moreover RB 885 886 , , , 887 896 tethering tethering NN 897 899 by by IN 900 910 P-selectin P-selectin NNP 911 923 specifically specifically RB 924 932 enhanced enhance VBD 933 940 nuclear nuclear JJ 941 954 translocation translocation NN 955 957 of of IN 958 965 nuclear nuclear JJ 966 978 factor-kappa factor-kappa NN 979 980 B B NNP 981 982 ( ( ( 982 990 NF-kappa NF-kappa NNP 991 992 B B NNP 992 993 ) ) ) 993 994 , , , 995 996 a a DT 997 1010 transcription transcription NN 1011 1017 factor factor NN 1018 1026 required require VBN 1027 1030 for for IN 1031 1041 expression expression NN 1042 1044 of of IN 1045 1050 MCP-1 mcp-1 NN 1050 1051 , , , 1052 1061 TNF-alpha TNF-alpha NNP 1061 1062 , , , 1063 1066 and and CC 1067 1072 other other JJ 1073 1088 immediate-early immediate-early JJ 1089 1094 genes gene NNS 1094 1095 . . . 1096 1101 These these DT 1102 1109 results result NNS 1110 1121 demonstrate demonstrate VBP 1122 1126 that that IN 1127 1137 P-selectin P-selectin NNP 1137 1138 , , , 1139 1146 through through IN 1147 1150 its its PRP$ 1151 1158 ligands ligand NNS 1159 1161 on on IN 1162 1171 monocytes monocyte NNS 1171 1172 , , , 1173 1176 may may MD 1177 1184 locally locally RB 1185 1193 regulate regulate VB 1194 1202 cytokine cytokine NN 1203 1212 secretion secretion NN 1213 1215 in in IN 1216 1224 inflamed inflamed JJ 1225 1232 tissues tissue NNS 1232 1233 . . .