0 12 Differential differential JJ 13 22 induction induction NN 23 25 of of IN 26 29 the the DT 30 35 NF-AT NF-AT NNP 36 43 complex complex NN 44 50 during during IN 51 64 restimulation restimulation NN 65 68 and and CC 69 72 the the DT 73 82 induction induction NN 83 85 of of IN 86 92 T-cell t-cell NN 93 99 anergy anergy NN 99 100 . . . 102 113 Stimulation stimulation NN 114 116 of of IN 117 122 human human JJ 123 127 CD4+ cd4+ JJ 128 134 T-cell t-cell NN 135 141 clones clone NNS 142 149 through through IN 150 153 the the DT 154 160 T-cell t-cell NN 161 169 receptor receptor NN 170 171 ( ( ( 171 174 TcR TcR NNP 174 175 ) ) ) 176 178 by by IN 179 183 high high JJ 184 189 doses dose NNS 190 192 of of IN 193 201 specific specific JJ 202 209 peptide peptide NN 210 217 results result VBZ 218 220 in in IN 221 224 the the DT 225 234 induction induction NN 235 237 of of IN 238 239 a a DT 240 250 long-lived long-lived JJ 251 256 state state NN 257 259 of of IN 260 277 nonresponsiveness nonresponsiveness NN 278 282 that that WDT 283 286 has have VBZ 287 291 been be VBN 292 298 called call VBN 299 305 anergy anergy NN 305 306 . . . 307 313 During during IN 314 317 the the DT 318 327 induction induction NN 328 330 of of IN 331 337 anergy anergy NN 337 338 , , , 339 340 T t NN 341 346 cells cell NNS 347 350 are be VBP 351 365 phenotypically phenotypically RB 366 373 similar similar JJ 374 376 to to TO 377 382 cells cell NNS 383 393 responding respond VBG 394 396 to to TO 397 399 an an DT 400 411 immunogenic immunogenic JJ 412 420 stimulus stimulus NN 420 421 . . . 422 425 The the DT 426 432 amount amount NN 433 435 of of IN 436 439 TcR tcr NN 440 442 at at IN 443 446 the the DT 447 451 cell cell NN 452 459 surface surface NN 460 462 is be VBZ 463 476 downmodulated downmodulate VBN 476 477 , , , 478 485 whereas whereas IN 486 489 the the DT 490 493 CD2 cd2 NN 494 497 and and CC 498 502 CD25 cd25 NN 503 512 receptors receptor NNS 513 516 are be VBP 517 526 increased increase VBN 526 527 . . . 528 532 When when WRB 533 545 restimulated restimulate VBN 545 546 , , , 547 554 however however RB 554 555 , , , 556 563 anergic anergic JJ 564 565 T t NN 566 571 cells cell NNS 572 576 fail fail VBP 577 579 to to TO 580 591 up-regulate up-regulate VB 592 605 transcription transcription NN 606 608 of of IN 609 612 the the DT 613 617 IL-2 il-2 NN 618 622 gene gene NN 623 626 and and CC 627 629 in in IN 630 641 consequence consequence NN 642 644 do do VBP 645 648 not not RB 649 656 produce produce VB 657 661 IL-2 il-2 NN 661 662 . . . 663 665 In in IN 666 670 this this DT 671 676 study study NN 676 677 , , , 678 680 we we PRP 681 685 have have VBP 686 694 compared compare VBN 695 698 the the DT 699 706 ability ability NN 707 709 of of IN 710 717 various various JJ 718 731 transcription transcription NN 732 739 factors factor NNS 740 742 to to TO 743 747 bind bind VB 748 750 to to TO 751 756 their their PRP$ 757 768 appropriate appropriate JJ 769 773 site site NN 774 776 on on IN 777 780 DNA DNA NNP 780 781 . . . 782 789 Factors factor NNS 790 794 were be VBD 795 803 isolated isolate VBN 804 808 from from IN 809 812 the the DT 813 819 nuclei nucleus NNS 820 822 of of IN 823 824 T t NN 825 830 cells cell NNS 831 835 that that WDT 836 840 were be VBD 841 843 in in IN 844 847 the the DT 848 857 induction induction NN 858 863 phase phase NN 864 866 of of IN 867 873 anergy anergy NN 874 876 or or CC 877 881 were be VBD 882 892 undergoing undergo VBG 893 903 activation activation NN 903 904 . . . 905 908 The the DT 909 916 pattern pattern NN 917 919 of of IN 920 927 binding binding NN 928 936 activity activity NN 937 939 in in IN 940 952 restimulated restimulate VBN 953 954 T t NN 955 960 cells cell NNS 961 963 is be VBZ 964 974 consistent consistent JJ 975 979 with with IN 980 983 the the DT 984 991 pattern pattern NN 992 996 that that WDT 997 1000 has have VBZ 1001 1011 previously previously RB 1012 1016 been be VBN 1017 1022 shown show VBN 1023 1025 to to TO 1026 1034 regulate regulate VB 1035 1050 T-cell-specific t-cell-specific JJ 1051 1061 expression expression NN 1062 1064 of of IN 1065 1068 the the DT 1069 1073 IL-2 il-2 NN 1074 1077 and and CC 1078 1081 the the DT 1082 1086 beta beta NN 1087 1092 chain chain NN 1093 1095 of of IN 1096 1099 the the DT 1100 1103 TcR tcr NN 1104 1109 genes gene NNS 1109 1110 . . . 1111 1114 The the DT 1115 1123 measured measure VBN 1124 1131 binding binding NN 1132 1134 to to TO 1135 1136 a a DT 1137 1142 TCF-1 tcf-1 NN 1143 1147 site site NN 1148 1150 is be VBZ 1151 1154 the the DT 1155 1159 same same JJ 1160 1162 in in IN 1163 1166 the the DT 1167 1173 nuclei nucleus NNS 1174 1176 of of IN 1177 1184 resting rest VBG 1184 1185 , , , 1186 1195 activated activate VBN 1195 1196 , , , 1197 1200 and and CC 1201 1210 anergized anergize VBN 1211 1216 cells cell NNS 1216 1217 . . . 1218 1221 The the DT 1222 1231 inducible inducible JJ 1232 1239 factors factor NNS 1240 1248 NK-kappa NK-kappa NNP 1249 1250 B B NNP 1250 1251 , , , 1252 1256 beta beta NN 1257 1259 E2 e2 NN 1259 1260 , , , 1261 1267 CD28RC CD28RC NNP 1267 1268 , , , 1269 1272 and and CC 1273 1277 AP-1 ap-1 NN 1278 1281 are be VBP 1282 1285 not not RB 1286 1295 expressed express VBN 1296 1298 in in IN 1299 1306 resting rest VBG 1307 1312 cells cell NNS 1313 1316 and and CC 1317 1320 are be VBP 1321 1328 twofold twofold RB 1329 1334 lower low JJR 1335 1337 in in IN 1338 1347 anergized anergize VBN 1348 1350 as as IN 1351 1359 compared compare VBN 1360 1364 with with IN 1365 1374 activated activate VBN 1375 1380 cells cell NNS 1380 1381 . . . 1382 1384 In in IN 1385 1393 contrast contrast NN 1393 1394 , , , 1395 1402 anergic anergic JJ 1403 1404 T t NN 1405 1410 cells cell NNS 1411 1418 express express VBP 1419 1432 approximately approximately RB 1433 1442 eightfold eightfold RB 1443 1448 lower low JJR 1449 1456 amounts amount NNS 1457 1459 of of IN 1460 1465 NF-AT NF-AT NNP 1465 1466 , , , 1467 1468 a a DT 1469 1475 member member NN 1476 1478 of of IN 1479 1482 the the DT 1483 1488 class class NN 1489 1491 of of IN 1492 1501 inducible inducible JJ 1502 1509 factors factor NNS 1510 1514 that that WDT 1515 1524 regulates regulate VBZ 1525 1529 IL-2 il-2 NN 1530 1534 gene gene NN 1535 1548 transcription transcription NN 1548 1549 . . . 1550 1551 ( ( ( 1551 1559 ABSTRACT abstract NN 1560 1569 TRUNCATED truncated VBN 1570 1572 AT at IN 1573 1576 250 250 CD 1577 1582 WORDS words NNS 1582 1583 ) ) )