0 12 Epstein-Barr epstein-barr JJ 13 18 virus virus NN 19 30 replicative replicative JJ 31 35 gene gene NN 36 49 transcription transcription NN 50 56 during during IN 57 59 de de FW 60 64 novo novo FW 65 74 infection infection NN 75 77 of of IN 78 83 human human JJ 84 94 thymocytes thymocyte NNS 94 95 : : : 96 108 simultaneous simultaneous JJ 109 114 early early JJ 115 125 expression expression NN 126 128 of of IN 129 135 BZLF-1 BZLF-1 NNP 136 139 and and CC 140 143 its its PRP$ 144 153 repressor repressor NN 154 157 RAZ RAZ NNP 157 158 . . . 160 172 Epstein-Barr epstein-barr JJ 173 178 virus virus NN 179 180 ( ( ( 180 183 EBV EBV NNP 183 184 ) ) ) 185 187 is be VBZ 188 193 known know VBN 194 196 to to TO 197 203 infect infect VB 204 205 B B NNP 206 211 cells cell NNS 212 215 and and CC 216 226 epithelial epithelial JJ 227 232 cells cell NNS 232 233 . . . 234 236 We we PRP 237 240 and and CC 241 247 others other NNS 248 252 have have VBP 253 258 shown show VBN 259 263 that that IN 264 267 EBV EBV NNP 268 271 can can MD 272 276 also also RB 277 283 infect infect VB 284 285 a a DT 286 292 subset subset NN 293 295 of of IN 296 306 thymocytes thymocyte NNS 306 307 . . . 308 317 Infection infection NN 318 320 of of IN 321 331 thymocytes thymocyte NNS 332 335 was be VBD 336 347 accompanied accompany VBN 348 350 by by IN 351 354 the the DT 355 365 appearance appearance NN 366 368 of of IN 369 375 linear linear JJ 376 379 EBV EBV NNP 380 386 genome genome NN 387 393 within within IN 394 395 8 8 CD 396 398 hr hr NN 399 401 of of IN 402 411 infection infection NN 411 412 . . . 413 428 Circularization circularization NN 429 431 of of IN 432 435 the the DT 436 439 EBV EBV NNP 440 446 genome genome NN 447 450 was be VBD 451 454 not not RB 455 463 detected detect VBN 463 464 . . . 465 469 This this DT 470 472 is be VBZ 473 475 in in IN 476 484 contrast contrast NN 485 487 to to TO 488 491 the the DT 492 501 infection infection NN 502 504 in in IN 505 506 B B NNP 507 512 cells cell NNS 513 518 where where WRB 519 522 the the DT 523 529 genome genome NN 530 533 can can MD 534 545 circularize circularize VB 546 552 within within IN 553 555 24 24 CD 556 558 hr hr NN 559 561 of of IN 562 571 infection infection NN 571 572 . . . 573 576 The the DT 577 587 appearance appearance NN 588 590 of of IN 591 594 the the DT 595 600 BamHI BamHI NNP 601 606 ZLF-1 zlf-1 NN 607 611 gene gene NN 612 619 product product NN 619 620 , , , 621 626 ZEBRA ZEBRA NNP 626 627 , , , 628 630 by by IN 631 637 RT-PCR RT-PCR NNP 637 638 , , , 639 642 was be VBD 643 651 observed observe VBN 652 658 within within IN 659 660 8 8 CD 661 663 hr hr NN 664 666 of of IN 667 676 infection infection NN 676 677 . . . 678 681 The the DT 682 692 appearance appearance NN 693 695 of of IN 696 697 a a DT 698 703 novel novel JJ 704 710 fusion fusion NN 711 721 transcript transcript NN 722 723 ( ( ( 723 726 RAZ RAZ NNP 726 727 ) ) ) 727 728 , , , 729 734 which which WDT 735 744 comprised comprise VBD 745 752 regions region NNS 753 755 of of IN 756 759 the the DT 760 766 BZLF-1 bzlf-1 NN 767 772 locus locus NN 773 776 and and CC 777 780 the the DT 781 789 adjacent adjacent JJ 790 796 BRLF-1 brlf-1 NN 797 802 locus locus NN 802 803 , , , 804 807 was be VBD 808 816 detected detect VBN 817 819 by by IN 820 826 RT-PCR RT-PCR NNP 826 827 . . . 828 833 ZEBRA ZEBRA NNP 834 841 protein protein NN 842 845 was be VBD 846 850 also also RB 851 861 identified identify VBN 862 864 in in IN 865 873 infected infected JJ 874 884 thymocytes thymocyte NNS 885 887 by by IN 888 907 immunoprecipitation immunoprecipitation NN 907 908 . . . 909 911 In in IN 912 920 addition addition NN 920 921 , , , 922 924 we we PRP 925 937 demonstrated demonstrate VBD 938 942 that that IN 943 946 the the DT 947 953 EBNA-1 ebna-1 NN 954 958 gene gene NN 959 961 in in IN 962 970 infected infected JJ 971 981 thymocytes thymocyte NNS 982 985 was be VBD 986 997 transcribed transcribe VBN 998 1002 from from IN 1003 1006 the the DT 1007 1009 Fp Fp NNP 1010 1018 promoter promoter NN 1018 1019 , , , 1020 1026 rather rather RB 1027 1031 than than IN 1032 1036 from from IN 1037 1040 the the DT 1041 1046 Cp/Wp Cp/Wp NNP 1047 1055 promoter promoter NN 1056 1061 which which WDT 1062 1064 is be VBZ 1065 1069 used use VBN 1070 1072 in in IN 1073 1081 latently latently RB 1082 1090 infected infected JJ 1091 1092 B B NNP 1093 1098 cells cell NNS 1098 1099 . . . 1100 1111 Transcripts transcript NNS 1112 1120 encoding encode VBG 1121 1130 gp350/220 gp350/220 NN 1130 1131 , , , 1132 1135 the the DT 1136 1141 major major JJ 1142 1146 coat coat NN 1147 1154 protein protein NN 1155 1157 of of IN 1158 1161 EBV EBV NNP 1161 1162 , , , 1163 1167 were be VBD 1168 1178 identified identify VBN 1178 1179 , , , 1180 1183 but but CC 1184 1186 we we PRP 1187 1190 did do VBD 1191 1194 not not RB 1195 1199 find find VB 1200 1203 any any DT 1204 1212 evidence evidence NN 1213 1215 of of IN 1216 1229 transcription transcription NN 1230 1234 from from IN 1235 1238 the the DT 1239 1245 LMP-2A LMP-2A NNP 1246 1248 or or CC 1249 1255 EBER-1 eber-1 NN 1256 1260 loci locus NNS 1261 1263 in in IN 1264 1272 infected infected JJ 1273 1283 thymocytes thymocyte NNS 1283 1284 . . . 1285 1290 These these DT 1291 1303 observations observation NNS 1304 1311 suggest suggest VBP 1312 1316 that that IN 1317 1319 de de FW 1320 1324 novo novo FW 1325 1328 EBV EBV NNP 1329 1338 infection infection NN 1339 1341 of of IN 1342 1352 thymocytes thymocyte NNS 1353 1360 differs differ VBZ 1361 1365 from from IN 1366 1375 infection infection NN 1376 1378 of of IN 1379 1380 B B NNP 1381 1386 cells cell NNS 1386 1387 . . . 1388 1391 The the DT 1392 1396 main main JJ 1397 1407 difference difference NN 1408 1410 is be VBZ 1411 1415 that that IN 1416 1420 with with IN 1421 1431 thymocytes thymocyte NNS 1431 1432 , , , 1433 1435 no no DT 1436 1444 evidence evidence NN 1445 1450 could could MD 1451 1453 be be VB 1454 1459 found find VBN 1460 1464 that that IN 1465 1468 the the DT 1469 1474 virus virus NN 1475 1479 ever ever RB 1480 1492 circularizes circularize VBZ 1492 1493 . . . 1494 1500 Rather rather RB 1500 1501 , , , 1502 1505 EBV EBV NNP 1506 1513 remains remain VBZ 1514 1516 in in IN 1517 1518 a a DT 1519 1525 linear linear JJ 1526 1539 configuration configuration NN 1540 1544 from from IN 1545 1550 which which WDT 1551 1562 replicative replicative JJ 1563 1568 genes gene NNS 1569 1572 are be VBP 1573 1584 transcribed transcribe VBN 1584 1585 . . .