0 9 Induction Induction NNP 10 12 of of IN 13 16 Sp1 sp1 NN 17 32 phosphorylation phosphorylation NN 33 36 and and CC 37 45 NF-kappa NF-kappa NNP 46 59 B-independent b-independent JJ 60 63 HIV HIV NNP 64 72 promoter promoter NN 73 79 domain domain NN 80 88 activity activity NN 89 91 in in IN 92 93 T t NN 94 105 lymphocytes lymphocyte NNS 106 116 stimulated stimulate VBN 117 119 by by IN 120 127 okadaic okadaic JJ 128 132 acid acid NN 132 133 . . . 135 137 In in IN 138 146 contrast contrast NN 147 149 to to TO 150 153 the the DT 154 160 purely purely RB 161 179 enhancer-dependent enhancer-dependent JJ 180 186 effect effect NN 187 189 of of IN 190 199 cytokines cytokine NNS 200 204 such such JJ 205 207 as as IN 208 211 TNF TNF NNP 212 214 on on IN 215 218 the the DT 219 227 activity activity NN 228 230 of of IN 231 234 the the DT 235 238 HIV HIV NNP 239 249 regulatory regulatory JJ 250 256 region region NN 257 258 ( ( ( 258 261 LTR LTR NNP 261 262 ) ) ) 262 263 , , , 264 266 we we PRP 267 275 observed observe VBD 276 280 that that IN 281 288 okadaic okadaic JJ 289 293 acid acid NN 294 295 ( ( ( 295 298 OKA OKA NNP 298 299 ) ) ) 300 309 activates activate VBZ 310 313 HIV HIV NNP 314 327 transcription transcription NN 328 335 through through IN 336 340 both both CC 341 344 the the DT 345 353 enhancer enhancer NN 353 354 , , , 355 365 responding respond VBG 366 368 to to TO 369 372 the the DT 373 379 factor factor NN 380 388 NF-kappa NF-kappa NNP 389 390 B B NNP 390 391 , , , 392 395 and and CC 396 399 the the DT 400 408 promoter promoter NN 409 415 domain domain NN 416 418 of of IN 419 422 the the DT 423 426 LTR ltr NN 426 427 . . . 428 431 The the DT 432 444 inducibility inducibility NN 445 447 of of IN 448 451 HIV HIV NNP 452 462 LTR-driven ltr-driven JJ 463 473 luciferase luciferase NN 474 484 expression expression NN 485 495 constructs construct NNS 496 498 in in IN 499 513 lymphoblastoid lymphoblastoid JJ 514 519 cells cell NNS 520 530 stimulated stimulate VBN 531 533 by by IN 534 537 OKA OKA NNP 538 546 depended depend VBD 547 549 on on IN 550 554 both both CC 555 565 functional functional JJ 566 569 Sp1 sp1 NN 570 577 binding binding NN 578 586 elements element NNS 587 590 and and CC 591 594 the the DT 595 602 ability ability NN 603 605 of of IN 606 609 the the DT 610 614 TATA TATA NNP 615 618 box box NN 619 621 to to TO 622 626 bind bind VB 627 630 the the DT 631 638 protein protein NN 639 642 TBP TBP NNP 642 643 . . . 644 646 In in IN 647 651 both both CC 652 663 transformed transform VBN 664 667 and and CC 668 674 normal normal JJ 675 686 lymphocytes lymphocyte NNS 686 687 , , , 688 691 OKA OKA NNP 692 703 stimulation stimulation NN 704 711 induced induce VBD 712 719 intense intense JJ 720 735 phosphorylation phosphorylation NN 736 738 of of IN 739 742 the the DT 743 757 constitutively constitutively RB 758 767 expressed express VBN 768 771 Sp1 sp1 NN 772 779 protein protein NN 780 782 in in IN 783 786 the the DT 787 794 nucleus nucleus NN 794 795 , , , 796 797 a a DT 798 806 property property NN 807 809 of of IN 810 813 OKA OKA NNP 814 817 not not RB 818 824 shared share VBN 825 827 by by IN 828 831 TNF TNF NNP 831 832 , , , 833 840 phorbol phorbol NN 841 846 ester ester NN 846 847 , , , 848 850 or or CC 851 854 PHA PHA NNP 855 858 and and CC 859 870 interleukin interleukin NN 871 872 2 2 CD 872 873 . . . 874 888 Responsiveness Responsiveness NNP 889 891 of of IN 892 895 LTR LTR NNP 896 906 constructs construct NNS 907 914 deleted delete VBN 915 917 of of IN 918 923 kappa kappa NN 924 925 B B NNP 926 934 elements element NNS 935 937 to to TO 938 941 HIV HIV NNP 942 945 Tat Tat NNP 946 956 expression expression NN 957 960 was be VBD 961 970 increased increase VBN 971 975 upon upon IN 976 979 OKA OKA NNP 980 983 but but CC 984 987 not not RB 988 991 TNF TNF NNP 992 1003 stimulation stimulation NN 1003 1004 . . . 1005 1008 Our our PRP$ 1009 1016 results result NNS 1017 1024 suggest suggest VBP 1025 1029 that that IN 1030 1033 SP1 sp1 NN 1034 1049 phosphorylation phosphorylation NN 1050 1057 induced induce VBN 1058 1060 by by IN 1061 1064 OKA OKA NNP 1064 1065 , , , 1066 1067 a a DT 1068 1077 selective selective JJ 1078 1087 inhibitor inhibitor NN 1088 1090 of of IN 1091 1094 the the DT 1095 1111 serine-threonine serine-threonine JJ 1112 1123 phosphatase phosphatase NN 1124 1128 PP2A PP2A NNP 1128 1129 , , , 1130 1141 facilitates facilitate VBZ 1142 1145 the the DT 1146 1155 formation formation NN 1156 1158 of of IN 1159 1160 a a DT 1161 1174 transcription transcription NN 1175 1182 complex complex NN 1183 1192 involving involve VBG 1193 1200 general general JJ 1201 1214 transcription transcription NN 1215 1222 factors factor NNS 1222 1223 , , , 1224 1227 HIV HIV NNP 1228 1231 Tat Tat NNP 1231 1232 , , , 1233 1236 and and CC 1237 1240 Sp1 sp1 NN 1241 1249 proteins protein NNS 1249 1250 . . . 1251 1254 The the DT 1255 1264 formation formation NN 1265 1267 of of IN 1268 1272 this this DT 1273 1280 complex complex NN 1281 1286 would would MD 1287 1295 increase increase VB 1295 1296 , , , 1297 1310 independently independently RB 1311 1313 of of IN 1314 1316 an an DT 1317 1319 in in IN 1320 1327 synergy synergy NN 1328 1332 with with IN 1333 1341 NF-kappa NF-kappa NNP 1342 1343 B B NNP 1343 1344 , , , 1345 1348 the the DT 1349 1352 low low JJ 1353 1358 basal basal JJ 1359 1367 activity activity NN 1368 1370 of of IN 1371 1374 the the DT 1375 1378 HIV HIV NNP 1379 1382 LTR LTR NNP 1383 1391 observed observe VBN 1392 1394 in in IN 1395 1401 normal normal JJ 1402 1403 T t NN 1404 1415 lymphocytes lymphocyte NNS 1415 1416 . . .