0 11 Calcineurin Calcineurin NNP 12 21 activates activate VBZ 22 35 transcription transcription NN 36 40 from from IN 41 44 the the DT 45 51 GM-CSF GM-CSF NNP 52 60 promoter promoter NN 61 63 in in IN 64 71 synergy synergy NN 72 76 with with IN 77 83 either either CC 84 91 protein protein NN 92 98 kinase kinase NNP 99 100 C C NNP 101 103 or or CC 104 112 NF-kappa NF-kappa NNP 113 119 B/AP-1 b/ap-1 NN 120 122 in in IN 123 124 T t NN 125 130 cells cell NNS 130 131 . . . 133 136 Two two CD 137 147 cis-acting cis-acting JJ 148 156 elements element NNS 157 165 GM-kappa GM-kappa NNP 166 174 B/GC-box B/GC-box NNP 175 178 and and CC 179 183 CLE0 CLE0 NNP 183 184 , , , 185 187 of of IN 188 191 the the DT 192 214 granulocyte-macrophage granulocyte-macrophage JJ 215 233 colony-stimulating colony-stimulating JJ 234 240 factor factor NN 241 242 ( ( ( 242 248 GM-CSF GM-CSF NNP 248 249 ) ) ) 250 254 gene gene NN 255 258 are be VBP 259 267 required require VBN 268 271 for for IN 272 279 maximal maximal JJ 280 289 induction induction NN 290 292 in in IN 293 299 Jurkat Jurkat NNP 300 301 T t NN 302 307 cells cell NNS 308 310 by by IN 311 324 costimulation costimulation NN 325 329 with with IN 330 350 phorbol-12-myristate phorbol-12-myristate NN 351 358 acetate acetate NN 359 360 ( ( ( 360 363 PMA PMA NNP 363 364 ) ) ) 365 368 and and CC 369 373 Ca2+ ca2+ NN 374 383 ionophore ionophore NN 384 385 ( ( ( 385 391 A23187 A23187 NNP 391 392 ) ) ) 392 393 . . . 394 397 The the DT 398 406 GM-kappa GM-kappa NNP 407 408 B B NNP 409 417 sequence sequence NN 418 420 is be VBZ 421 431 recognized recognize VBN 432 434 by by IN 435 443 NF-kappa NF-kappa NNP 444 445 B B NNP 445 446 , , , 447 452 which which WDT 453 455 is be VBZ 456 462 mainly mainly RB 463 470 induced induce VBN 471 473 by by IN 474 477 PMA PMA NNP 477 478 . . . 479 482 The the DT 483 487 CLE0 cle0 NN 488 496 sequence sequence NN 497 506 interacts interact VBZ 507 511 with with IN 512 519 factors factor NNS 519 520 , , , 521 528 related related JJ 529 531 to to TO 532 533 a a DT 534 545 PMA-induced pma-induced JJ 546 550 AP-1 ap-1 NN 551 554 and and CC 555 556 a a DT 557 575 PMA/A23187-induced pma/a23187-induced JJ 576 581 NF-AT NF-AT NNP 581 582 . . . 583 585 We we PRP 586 594 examined examine VBD 595 602 whether whether IN 603 609 signal signal NN 610 621 transducing transducing NN 622 632 components component NNS 633 635 in in IN 636 637 T t NN 638 643 cells cell NNS 644 647 can can MD 648 656 activate activate VB 657 670 transcription transcription NN 671 673 of of IN 674 677 the the DT 678 684 GM-CSF GM-CSF NNP 685 689 gene gene NN 689 690 . . . 691 705 Cotransfection Cotransfection NNP 706 708 of of IN 709 717 NF-kappa NF-kappa NNP 718 719 B B NNP 720 721 ( ( ( 721 730 p50/p65)- p50/p65)- NN 731 733 or or CC 734 738 AP-1 ap-1 NN 739 740 ( ( ( 740 753 c-Jun/c-Fos)- c-jun/c-fos)- NN 754 764 expression expression NN 765 772 vectors vector NNS 773 777 into into IN 778 784 Jurkat Jurkat NNP 785 790 cells cell NNS 791 795 with with IN 796 797 a a DT 798 808 luciferase luciferase NN 809 817 reporter reporter NN 818 828 containing contain VBG 829 832 the the DT 833 839 GM-CSF GM-CSF NNP 840 848 promoter promoter NN 849 852 did do VBD 853 856 not not RB 857 866 stimulate stimulate VB 867 880 transcription transcription NN 881 885 from from IN 886 889 the the DT 890 896 GM-CSF GM-CSF NNP 897 905 promoter promoter NN 905 906 . . . 907 909 In in IN 910 918 contrast contrast NN 918 919 , , , 920 934 cotransfection cotransfection NN 935 939 with with IN 940 941 a a DT 942 953 combination combination NN 954 956 of of IN 957 965 NF-kappa NF-kappa NNP 966 967 B B NNP 968 971 and and CC 972 976 AP-1 AP-1 NNP 977 990 significantly significantly RB 991 1000 augmented augment VBD 1001 1014 transcription transcription NN 1015 1019 from from IN 1020 1023 the the DT 1024 1030 GM-CSF GM-CSF NNP 1031 1039 promoter promoter NN 1040 1050 containing contain VBG 1051 1054 the the DT 1055 1063 GM-kappa GM-kappa NNP 1064 1072 B/GC-box B/GC-box NNP 1073 1076 and and CC 1077 1080 the the DT 1081 1085 CLE0 CLE0 NNP 1086 1087 ( ( ( 1087 1097 AP-1/NF-AT AP-1/NF-AT NNP 1097 1098 ) ) ) 1098 1099 . . . 1100 1110 Expression expression NN 1111 1113 of of IN 1114 1115 a a DT 1116 1130 constitutively constitutively RB 1131 1137 active active JJ 1138 1149 calcineurin calcineurin NN 1150 1151 ( ( ( 1151 1153 CN CN NNP 1153 1154 ) ) ) 1154 1155 , , , 1156 1157 a a DT 1158 1183 Ca2+/calmodulin-dependent ca2+/calmodulin-dependent JJ 1184 1191 protein protein NN 1192 1203 phosphatase phosphatase NN 1203 1204 , , , 1205 1216 potentiated potentiate VBN 1217 1219 by by IN 1220 1223 two two CD 1224 1228 fold fold RB 1229 1232 the the DT 1233 1248 transcriptional transcriptional JJ 1249 1259 activation activation NN 1260 1262 by by IN 1263 1271 NF-kappa NF-kappa NNP 1272 1278 B/AP-1 b/ap-1 NN 1278 1279 . . . 1280 1284 Both both CC 1285 1299 constitutively constitutively RB 1300 1306 active active JJ 1307 1312 forms form NNS 1313 1315 of of IN 1316 1318 CN CN NNP 1319 1322 and and CC 1323 1330 protein protein NN 1331 1337 kinase kinase NNP 1338 1339 C C NNP 1340 1341 ( ( ( 1341 1344 PKC PKC NNP 1344 1345 ) ) ) 1346 1361 synergistically synergistically RB 1362 1371 activated activate VBD 1372 1385 transcription transcription NN 1386 1390 from from IN 1391 1394 the the DT 1395 1401 GM-CSF GM-CSF NNP 1402 1410 promoter promoter NN 1410 1411 . . . 1412 1417 These these DT 1418 1425 results result NNS 1426 1433 suggest suggest VBP 1434 1438 that that IN 1439 1450 cooperation cooperation NN 1451 1456 among among IN 1457 1465 NF-kappa NF-kappa NNP 1466 1468 B- B- NNP 1468 1469 , , , 1470 1475 AP-1- AP-1- NNP 1476 1479 and and CC 1480 1493 NF-AT-binding nf-at-binding JJ 1494 1503 sequences sequence NNS 1504 1506 is be VBZ 1507 1515 required require VBN 1516 1519 for for IN 1520 1529 induction induction NN 1530 1532 of of IN 1533 1536 the the DT 1537 1543 GM-CSF GM-CSF NNP 1544 1548 gene gene NN 1549 1556 through through IN 1557 1561 PKC- PKC- NNP 1562 1565 and and CC 1566 1571 Ca2+- ca2+- NN 1572 1581 signaling signaling NN 1582 1590 pathways pathway NNS 1591 1601 downstream downstream JJ 1602 1604 of of IN 1605 1606 T t NN 1607 1611 cell cell NN 1612 1622 activation activation NN 1622 1623 . . .