0 13 Cell-specific cell-specific JJ 14 26 bifunctional bifunctional JJ 27 31 role role NN 32 34 of of IN 35 38 Jun Jun NNP 39 47 oncogene oncogene NN 48 54 family family NN 55 62 members member NNS 63 65 on on IN 66 80 glucocorticoid glucocorticoid NN 81 99 receptor-dependent receptor-dependent JJ 100 113 transcription transcription NN 113 114 . . . 116 127 Interaction interaction NN 128 135 between between IN 136 143 protein protein NN 144 150 kinase kinase NNP 151 152 C C NNP 153 154 ( ( ( 154 159 PKC)- PKC)- NNP 160 163 and and CC 164 178 glucocorticoid glucocorticoid NN 179 187 receptor receptor NN 188 189 ( ( ( 189 201 GR)-mediated gr)-mediated JJ 202 211 signaling signaling NN 212 214 is be VBZ 215 224 suggested suggest VBN 225 227 by by IN 228 231 the the DT 232 239 ability ability NN 240 242 of of IN 243 246 the the DT 247 250 PKC PKC NNP 251 261 activating activate VBG 262 269 phorbol phorbol NN 270 275 ester ester NN 276 312 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate NNP 313 314 ( ( ( 314 317 TPA TPA NNP 317 318 ) ) ) 319 321 to to TO 322 329 inhibit inhibit VB 330 342 GR-dependent gr-dependent JJ 343 356 transcription transcription NN 357 359 of of IN 360 363 the the DT 364 369 mouse mouse NN 370 377 mammary mammary JJ 378 383 tumor tumor NN 384 389 virus virus NN 390 391 ( ( ( 391 395 MMTV MMTV NNP 395 396 ) ) ) 397 401 long long JJ 402 410 terminal terminal JJ 411 417 repeat repeat NN 418 419 ( ( ( 419 422 LTR LTR NNP 422 423 ) ) ) 423 424 . . . 425 429 Here here RB 430 432 we we PRP 433 439 report report VBP 440 444 that that IN 445 449 this this DT 450 462 interference interference NN 463 465 is be VBZ 466 470 cell cell NN 471 479 specific specific JJ 479 480 , , , 481 483 as as IN 484 487 TPA TPA NNP 488 497 augmented augment VBD 498 519 dexamethasone-induced dexamethasone-induced JJ 520 535 transcriptional transcriptional JJ 536 546 activation activation NN 547 549 of of IN 550 553 the the DT 554 558 MMTV MMTV NNP 559 562 LTR LTR NNP 563 565 in in IN 566 573 several several JJ 574 575 T t NN 576 580 cell cell NN 581 586 lines line NNS 587 590 but but CC 591 594 was be VBD 595 605 inhibitory inhibitory JJ 606 608 in in IN 609 616 NIH-3T3 nih-3t3 NN 617 628 fibroblasts fibroblast NNS 628 629 . . . 630 636 TPA-GR TPA-GR NNP 637 646 synergism synergism NN 647 650 was be VBD 651 661 determined determine VBN 662 664 to to TO 665 669 have have VB 670 678 occurred occur VBN 679 681 at at IN 682 685 the the DT 686 699 GR-responsive gr-responsive JJ 700 707 element element NN 708 709 ( ( ( 709 712 GRE GRE NNP 712 713 ) ) ) 714 719 level level NN 720 722 by by IN 723 733 functional functional JJ 734 742 analysis analysis NN 743 745 of of IN 746 754 deletion deletion NN 755 762 mutants mutant NNS 763 765 or or CC 766 775 synthetic synthetic JJ 776 779 GRE GRE NNP 780 796 oligonucleotides oligonucleotide NNS 797 804 driving drive VBG 805 820 chloramphenicol chloramphenicol NN 821 839 acetyl-transferase acetyl-transferase NN 840 850 expression expression NN 850 851 . . . 852 861 Synergism Synergism NNP 862 870 required require VBD 871 873 an an DT 874 880 intact intact JJ 881 883 GR gr NN 884 895 DNA-binding dna-binding JJ 896 902 domain domain NN 902 903 , , , 904 911 whereas whereas IN 912 918 amino- amino- NN 919 921 or or CC 922 939 carboxyl-terminal carboxyl-terminal JJ 940 947 domains domain NNS 948 952 were be VBD 953 964 dispensable dispensable JJ 964 965 . . . 966 969 The the DT 970 976 effect effect NN 977 980 was be VBD 981 990 abrogated abrogate VBN 991 993 by by IN 994 997 the the DT 998 1001 PKC PKC NNP 1002 1011 inhibitor inhibitor NN 1012 1025 staurosporine staurosporine NN 1025 1026 , , , 1027 1037 suggesting suggest VBG 1038 1039 a a DT 1040 1044 role role NN 1045 1048 for for IN 1049 1052 PKC PKC NNP 1052 1053 . . . 1054 1063 Increased increase VBN 1064 1069 c-jun c-jun NN 1069 1070 , , , 1071 1076 jun-B jun-b NN 1076 1077 , , , 1078 1081 and and CC 1082 1087 jun-D jun-d NN 1088 1098 expression expression NN 1099 1104 above above IN 1105 1110 basal basal JJ 1111 1117 levels level NNS 1118 1121 and and CC 1122 1131 increased increase VBN 1132 1147 transcriptional transcriptional JJ 1148 1156 activity activity NN 1157 1159 of of IN 1160 1168 AP-1/TPA AP-1/TPA NNP 1169 1179 responsive responsive JJ 1180 1188 elements element NNS 1189 1194 fused fuse VBN 1195 1197 to to TO 1198 1213 chloramphenicol chloramphenicol NN 1214 1232 acetyl-transferase acetyl-transferase NN 1233 1240 vectors vector NNS 1241 1245 were be VBD 1246 1254 observed observe VBN 1255 1257 in in IN 1258 1259 T t NN 1260 1265 cells cell NNS 1266 1273 treated treat VBN 1274 1278 with with IN 1279 1282 TPA TPA NNP 1283 1288 alone alone RB 1289 1291 or or CC 1292 1294 in in IN 1295 1306 combination combination NN 1307 1311 with with IN 1312 1325 dexamethasone dexamethasone NN 1325 1326 . . . 1327 1330 The the DT 1331 1338 ability ability NN 1339 1341 of of IN 1342 1345 Jun Jun NNP 1346 1354 proteins protein NNS 1355 1357 to to TO 1358 1367 cooperate cooperate VB 1368 1372 with with IN 1373 1375 GR gr NN 1376 1378 in in IN 1379 1380 T t NN 1381 1386 cells cell NNS 1387 1390 has have VBZ 1391 1395 been be VBN 1396 1408 investigated investigate VBN 1409 1414 after after IN 1415 1427 transfection transfection NN 1428 1430 of of IN 1431 1436 c-jun c-jun NN 1436 1437 , , , 1438 1443 jun-B jun-b NN 1443 1444 , , , 1445 1447 or or CC 1448 1453 jun-D jun-d NN 1454 1464 expression expression NN 1465 1472 vectors vector NNS 1472 1473 , , , 1474 1479 which which WDT 1480 1489 augmented augment VBD 1490 1502 GR-dependent gr-dependent JJ 1503 1516 transcription transcription NN 1517 1521 from from IN 1522 1528 either either CC 1529 1533 MMTV MMTV NNP 1534 1537 LTR LTR NNP 1538 1540 or or CC 1541 1544 GRE GRE NNP 1544 1545 . . . 1546 1556 Conversely conversely RB 1556 1557 , , , 1558 1563 c-jun c-jun NN 1564 1567 and and CC 1568 1573 jun-B jun-b NN 1574 1586 transfection transfection NN 1587 1594 blunted blunt VBD 1595 1607 GR-dependent gr-dependent JJ 1608 1621 transcription transcription NN 1622 1624 in in IN 1625 1629 HeLa HeLa NNP 1630 1635 cells cell NNS 1635 1636 . . . 1637 1640 The the DT 1641 1649 presence presence NN 1650 1652 of of IN 1653 1658 c-fos c-fos NN 1659 1662 had have VBD 1663 1664 a a DT 1665 1673 negative negative JJ 1674 1683 influence influence NN 1684 1686 on on IN 1687 1689 GR GR NNP 1690 1698 function function NN 1699 1702 and and CC 1703 1713 correlated correlate VBD 1714 1718 with with IN 1719 1722 the the DT 1723 1736 cell-specific cell-specific JJ 1737 1748 synergistic synergistic JJ 1749 1751 or or CC 1752 1764 antagonistic antagonistic JJ 1765 1773 activity activity NN 1774 1776 of of IN 1777 1780 Jun Jun NNP 1781 1785 with with IN 1786 1793 respect respect NN 1794 1796 to to TO 1797 1799 GR GR NNP 1799 1800 ; ; : 1801 1805 high high JJ 1806 1811 basal basal JJ 1812 1822 expression expression NN 1823 1825 of of IN 1826 1831 c-fos c-fos NN 1832 1834 as as RB 1835 1839 well well RB 1840 1842 as as IN 1843 1847 AP-1 ap-1 NN 1848 1851 DNA dna NN 1852 1859 binding binding NN 1860 1863 and and CC 1864 1879 transcriptional transcriptional JJ 1880 1888 activity activity NN 1889 1893 were be VBD 1894 1902 observed observe VBN 1903 1905 in in IN 1906 1910 HeLa HeLa NNP 1911 1916 cells cell NNS 1916 1917 , , , 1918 1921 but but CC 1922 1925 not not RB 1926 1928 in in IN 1929 1930 T t NN 1931 1936 cells cell NNS 1936 1937 . . . 1938 1949 Furthermore furthermore RB 1950 1964 overexpression overexpression NN 1965 1967 of of IN 1968 1977 exogenous exogenous JJ 1978 1983 c-fos c-fos NN 1984 1987 has have VBZ 1988 1990 an an DT 1991 2001 inhibitory inhibitory JJ 2002 2008 effect effect NN 2009 2011 on on IN 2012 2024 GR-dependent gr-dependent JJ 2025 2038 transcription transcription NN 2039 2043 from from IN 2044 2047 GRE GRE NNP 2048 2050 in in IN 2051 2052 T t NN 2053 2058 cells cell NNS 2058 2059 . . . 2060 2062 We we PRP 2063 2070 propose propose VBP 2071 2075 that that IN 2076 2079 Jun Jun NNP 2080 2085 plays play VBZ 2086 2087 a a DT 2088 2100 bifunctional bifunctional JJ 2101 2105 role role NN 2106 2108 on on IN 2109 2121 GR-dependent gr-dependent JJ 2122 2137 transcriptional transcriptional JJ 2138 2148 activation activation NN 2149 2151 of of IN 2152 2155 GRE GRE NNP 2155 2156 , , , 2157 2166 selecting select VBG 2167 2173 either either CC 2174 2185 synergistic synergistic JJ 2186 2188 or or CC 2189 2201 antagonistic antagonistic JJ 2202 2210 activity activity NN 2211 2220 depending depend VBG 2221 2223 on on IN 2224 2227 the the DT 2228 2241 cell-specific cell-specific JJ 2242 2258 microenvironment microenvironment NN 2258 2259 . . . 2260 2262 In in IN 2263 2267 this this DT 2268 2274 regard regard NN 2274 2275 , , , 2276 2289 intracellular intracellular JJ 2290 2296 levels level NNS 2297 2299 of of IN 2300 2305 c-fos c-fos NN 2306 2312 appear appear VBP 2313 2315 to to TO 2316 2318 be be VB 2319 2330 influential influential JJ 2330 2331 . . .