0 10 Initiation initiation NN 11 18 binding binding NN 19 28 repressor repressor NN 28 29 , , , 30 31 a a DT 32 38 factor factor NN 39 43 that that WDT 44 49 binds bind VBZ 50 52 to to TO 53 56 the the DT 57 70 transcription transcription NN 71 81 initiation initiation NN 82 86 site site NN 87 89 of of IN 90 93 the the DT 94 101 histone histone NN 102 104 h5 h5 NN 105 109 gene gene NN 109 110 , , , 111 113 is be VBZ 114 115 a a DT 116 128 glycosylated glycosylate VBN 129 135 member member NN 136 138 of of IN 139 140 a a DT 141 147 family family NN 148 150 of of IN 151 155 cell cell NN 156 162 growth growth NN 163 173 regulators regulator NNS 174 175 [ [ ( 175 184 corrected correct VBN 184 185 ] ] ) 186 187 [ [ ( 187 196 published publish VBN 197 204 erratum erratum NN 205 212 appears appear VBZ 213 215 in in IN 216 219 Mol Mol NNP 220 224 Cell Cell NNP 225 229 Biol Biol NNP 230 234 1996 1996 CD 235 248 Feb;16(2):735 feb;16(2):735 . 248 249 ] ] ) 251 261 Initiation Initiation NNP 262 269 binding binding NN 270 279 repressor repressor NN 280 281 [ [ ( 281 290 corrected correct VBN 290 291 ] ] ) 292 293 ( ( ( 293 296 IBR IBR NNP 296 297 ) ) ) 298 300 is be VBZ 301 302 a a DT 303 310 chicken chicken NN 311 322 erythrocyte erythrocyte NN 323 329 factor factor NN 330 331 ( ( ( 331 339 apparent apparent JJ 340 349 molecular molecular JJ 350 354 mass mass NN 354 355 , , , 356 358 70 70 CD 359 361 to to TO 362 364 73 73 CD 365 368 kDa kda NN 368 369 ) ) ) 370 374 that that WDT 375 380 binds bind VBZ 381 383 to to TO 384 387 the the DT 388 397 sequences sequence NNS 398 406 spanning span VBG 407 410 the the DT 411 424 transcription transcription NN 425 435 initiation initiation NN 436 440 site site NN 441 443 of of IN 444 447 the the DT 448 455 histone histone NN 456 458 h5 h5 NN 459 463 gene gene NN 463 464 , , , 465 475 repressing repress VBG 476 479 its its PRP$ 480 493 transcription transcription NN 493 494 . . . 495 496 A a DT 497 504 variety variety NN 505 507 of of IN 508 513 other other JJ 514 519 cells cell NNS 519 520 , , , 521 530 including include VBG 531 542 transformed transform VBN 543 552 erythroid erythroid JJ 553 563 precursors precursor NNS 563 564 , , , 565 567 do do VBP 568 571 not not RB 572 576 have have VB 577 580 IBR IBR NNP 581 584 but but CC 585 586 a a DT 587 593 factor factor NN 594 602 referred refer VBN 603 605 to to TO 606 608 as as IN 609 612 IBF IBF NNP 613 614 ( ( ( 614 616 68 68 CD 617 619 to to TO 620 622 70 70 CD 623 626 kDa kda NN 626 627 ) ) ) 628 632 that that WDT 633 643 recognizes recognize VBZ 644 647 the the DT 648 652 same same JJ 653 656 IBR ibr NN 657 662 sites site NNS 662 663 . . . 664 666 We we PRP 667 671 have have VBP 672 678 cloned clone VBN 679 682 the the DT 683 686 IBR IBR NNP 687 691 cDNA cDNA NNP 692 695 and and CC 696 703 studied study VBD 704 707 the the DT 708 720 relationship relationship NN 721 723 of of IN 724 727 IBR IBR NNP 728 731 and and CC 732 735 IBF IBF NNP 735 736 . . . 737 740 IBR IBR NNP 741 743 is be VBZ 744 745 a a DT 746 765 503-amino-acid-long 503-amino-acid-long JJ 766 772 acidic acidic JJ 773 780 protein protein NN 781 786 which which WDT 787 789 is be VBZ 790 794 99.0 99.0 CD 794 795 % % NN 796 805 identical identical JJ 806 808 to to TO 809 812 the the DT 813 821 recently recently RB 822 830 reported report VBN 831 836 human human JJ 837 852 NRF-1/alpha-Pal nrf-1/alpha-pal JJ 853 859 factor factor NN 860 863 and and CC 864 870 highly highly RB 871 878 related related JJ 879 881 to to TO 882 885 the the DT 886 898 invertebrate invertebrate NN 899 912 transcription transcription NN 913 920 factors factor NNS 921 925 P3A2 p3a2 NN 926 929 and and CC 930 937 erected erect VBN 938 942 wing wing NN 943 947 gene gene NN 948 955 product product NN 956 957 ( ( ( 957 960 EWG EWG NNP 960 961 ) ) ) 961 962 . . . 963 965 We we PRP 966 973 present present VBP 974 982 evidence evidence NN 983 987 that that IN 988 991 IBR IBR NNP 992 995 and and CC 996 999 IBF IBF NNP 1000 1003 are be VBP 1004 1008 most most RBS 1009 1015 likely likely JJ 1016 1025 identical identical JJ 1026 1034 proteins protein NNS 1034 1035 , , , 1036 1045 differing differ VBG 1046 1048 in in IN 1049 1054 their their PRP$ 1055 1061 degree degree NN 1062 1064 of of IN 1065 1078 glycosylation glycosylation NN 1078 1079 . . . 1080 1082 We we PRP 1083 1087 have have VBP 1088 1096 analyzed analyze VBN 1097 1104 several several JJ 1105 1114 molecular molecular JJ 1115 1122 aspects aspect NNS 1123 1125 of of IN 1126 1131 IBR/F IBR/F NNP 1132 1135 and and CC 1136 1141 shown show VBN 1142 1146 that that IN 1147 1150 the the DT 1151 1157 factor factor NN 1158 1168 associates associate VBZ 1169 1171 as as IN 1172 1178 stable stable JJ 1179 1189 homodimers homodimer NNS 1190 1193 and and CC 1194 1198 that that IN 1199 1202 the the DT 1203 1208 dimer dimer NN 1209 1211 is be VBZ 1212 1215 the the DT 1216 1224 relevant relevant JJ 1225 1236 DNA-binding dna-binding JJ 1237 1244 species specie NNS 1244 1245 . . . 1246 1249 The the DT 1250 1264 evolutionarily evolutionarily RB 1265 1274 conserved conserve VBN 1275 1285 N-terminal n-terminal JJ 1286 1290 half half NN 1291 1293 of of IN 1294 1299 IBR/F IBR/F NNP 1300 1307 harbors harbor VBZ 1308 1311 the the DT 1312 1336 DNA-binding/dimerization dna-binding/dimerization NN 1337 1343 domain domain NN 1344 1345 ( ( ( 1345 1350 outer outer JJ 1351 1357 limits limit NNS 1357 1358 , , , 1359 1362 127 127 CD 1363 1365 to to TO 1366 1369 283 283 CD 1369 1370 ) ) ) 1370 1371 , , , 1372 1375 one one CD 1376 1378 or or CC 1379 1386 several several JJ 1387 1393 casein casein NN 1394 1400 kinase kinase NNP 1401 1403 II ii CD 1404 1409 sites site NNS 1410 1411 ( ( ( 1411 1413 37 37 CD 1414 1416 to to TO 1417 1419 67 67 CD 1419 1420 ) ) ) 1420 1421 , , , 1422 1425 and and CC 1426 1427 a a DT 1428 1437 bipartite bipartite JJ 1438 1445 nuclear nuclear JJ 1446 1458 localization localization NN 1459 1465 signal signal NN 1466 1467 ( ( ( 1467 1469 89 89 CD 1470 1472 to to TO 1473 1476 106 106 CD 1476 1477 ) ) ) 1478 1483 which which WDT 1484 1491 appears appear VBZ 1492 1494 to to TO 1495 1497 be be VB 1498 1507 necessary necessary JJ 1508 1511 for for IN 1512 1519 nuclear nuclear JJ 1520 1529 targeting targeting NN 1529 1530 . . . 1531 1538 Binding binding NN 1539 1543 site site NN 1544 1553 selection selection NN 1554 1562 revealed reveal VBD 1563 1567 that that IN 1568 1571 the the DT 1572 1583 alternating alternate VBG 1584 1594 RCGCRYGCGY rcgcrygcgy NN 1595 1604 consensus consensus NN 1605 1616 constitutes constitute VBZ 1617 1630 high-affinity high-affinity JJ 1631 1636 IBR/F ibr/f NN 1637 1644 binding binding NN 1645 1650 sites site NNS 1651 1654 and and CC 1655 1659 that that IN 1660 1663 the the DT 1664 1677 direct-repeat direct-repeat JJ 1678 1688 palindrome palindrome NN 1689 1701 TGCGCATGCGCA TGCGCATGCGCA NNP 1702 1704 is be VBZ 1705 1708 the the DT 1709 1716 optimal optimal JJ 1717 1721 site site NN 1721 1722 . . . 1723 1724 A a DT 1725 1731 survey survey NN 1732 1734 of of IN 1735 1740 genes gene NNS 1741 1752 potentially potentially RB 1753 1762 regulated regulate VBN 1763 1765 by by IN 1766 1770 this this DT 1771 1777 family family NN 1778 1780 of of IN 1781 1788 factors factor NNS 1789 1798 primarily primarily RB 1799 1807 revealed reveal VBD 1808 1813 genes gene NNS 1814 1822 involved involve VBN 1823 1825 in in IN 1826 1840 growth-related growth-related JJ 1841 1851 metabolism metabolism NN 1851 1852 . . .