0 15 Transcriptional transcriptional JJ 16 26 regulation regulation NN 27 29 of of IN 30 33 the the DT 34 47 interleukin-2 interleukin-2 NN 48 52 gene gene NN 53 55 in in IN 56 62 normal normal JJ 63 68 human human JJ 69 79 peripheral peripheral JJ 80 85 blood blood NN 86 87 T t NN 88 93 cells cell NNS 93 94 . . . 95 106 Convergence Convergence NNP 107 109 of of IN 110 123 costimulatory costimulatory JJ 124 131 signals signal NNS 132 135 and and CC 136 147 differences difference NNS 148 152 from from IN 153 164 transformed transform VBN 165 166 T t NN 167 172 cells cell NNS 172 173 . . . 175 177 To to TO 178 183 study study VB 184 199 transcriptional transcriptional JJ 200 210 regulation regulation NN 211 213 in in IN 214 220 normal normal JJ 221 226 human human JJ 227 228 T t NN 229 234 cells cell NNS 234 235 , , , 236 238 we we PRP 239 243 have have VBP 244 253 optimized optimize VBN 254 264 conditions condition NNS 265 268 for for IN 269 278 transient transient JJ 279 291 transfection transfection NN 291 292 . . . 293 306 Interleukin-2 interleukin-2 NN 307 308 ( ( ( 308 312 IL-2 il-2 NN 312 313 ) ) ) 314 331 promoter-reporter promoter-reporter JJ 332 336 gene gene NN 337 345 behavior behavior NN 346 353 closely closely RB 354 363 parallels parallel VBZ 364 367 the the DT 368 378 endogenous endogenous JJ 379 383 gene gene NN 384 386 in in IN 387 395 response response NN 396 398 to to TO 399 400 T t NN 401 405 cell cell NN 406 414 receptor receptor NN 415 418 and and CC 419 432 costimulatory costimulatory JJ 433 440 signals signal NNS 440 441 . . . 442 444 As as IN 445 453 assessed assess VBN 454 458 with with IN 459 470 mutagenized mutagenize VBN 471 480 promoters promoter NNS 480 481 , , , 482 485 the the DT 486 490 most most RBS 491 500 important important JJ 501 505 IL-2 il-2 NN 506 520 cis-regulatory cis-regulatory JJ 521 529 elements element NNS 530 532 in in IN 533 539 normal normal JJ 540 541 T t NN 542 547 cells cell NNS 548 551 are be VBP 552 555 the the DT 556 564 proximal proximal JJ 565 569 AP-1 ap-1 NN 570 574 site site NN 575 578 and and CC 579 582 the the DT 583 586 NF- nf- NF- 587 593 kappaB kappab NN 594 598 site site NN 598 599 . . . 600 604 Both both CC 605 612 primary primary JJ 613 623 activation activation NN 623 624 , , , 625 629 with with IN 630 648 phytohemagglutinin phytohemagglutinin NN 649 651 or or CC 652 662 antibodies antibody NNS 663 665 to to TO 666 669 CD3 CD3 NNP 669 670 , , , 671 674 and and CC 675 688 costimulation costimulation NN 688 689 , , , 690 698 provided provide VBN 699 701 by by IN 702 707 pairs pair NNS 708 710 of of IN 711 714 CD2 cd2 NN 715 725 antibodies antibody NNS 726 728 or or CC 729 740 B7-positive b7-positive JJ 741 742 ( ( ( 742 743 B B NNP 744 749 cells cell NNS 749 750 ) ) ) 751 753 or or CC 754 765 B7-negative b7-negative JJ 766 767 ( ( ( 767 778 endothelial endothelial JJ 778 779 ) ) ) 780 789 accessory accessory JJ 790 795 cells cell NNS 795 796 , , , 797 800 are be VBP 801 809 mediated mediate VBN 810 817 through through IN 818 821 the the DT 822 826 same same JJ 827 839 cis-elements cis-element NNS 839 840 . . . 841 854 Interestingly interestingly RB 854 855 , , , 856 859 the the DT 860 867 nuclear nuclear JJ 868 874 factor factor NN 875 877 of of IN 878 887 activated activate VBN 888 889 T t NN 890 894 cell cell NN 895 900 sites site NNS 901 904 are be VBP 905 909 much much RB 910 914 less less RBR 915 924 important important JJ 925 927 in in IN 928 934 normal normal JJ 935 936 T t NN 937 942 cells cell NNS 943 947 than than IN 948 950 in in IN 951 957 Jurkat Jurkat NNP 958 959 T t NN 960 965 cells cell NNS 965 966 . . . 967 969 We we PRP 970 978 conclude conclude VBP 979 983 that that IN 984 988 IL-2 il-2 NN 989 1004 transcriptional transcriptional JJ 1005 1015 regulation regulation NN 1016 1023 differs differ VBZ 1024 1026 in in IN 1027 1032 tumor tumor NN 1033 1037 cell cell NN 1038 1043 lines line NNS 1044 1052 compared compare VBN 1053 1057 with with IN 1058 1064 normal normal JJ 1065 1066 T t NN 1067 1072 cells cell NNS 1073 1076 and and CC 1077 1081 that that IN 1082 1091 different different JJ 1092 1105 costimulatory costimulatory JJ 1106 1113 signals signal NNS 1114 1122 converge converge VBP 1123 1125 on on IN 1126 1129 the the DT 1130 1134 same same JJ 1135 1147 cis-elements cis-element NNS 1148 1150 in in IN 1151 1154 the the DT 1155 1159 IL-2 il-2 NN 1160 1168 promoter promoter NN 1168 1169 . . .