0 12 Interactions interaction NNS 13 15 of of IN 16 17 a a DT 18 33 transcriptional transcriptional JJ 34 43 activator activator NN 44 46 in in IN 47 50 the the DT 51 54 env env NN 55 59 gene gene NN 60 62 of of IN 63 66 the the DT 67 72 mouse mouse NN 73 80 mammary mammary JJ 81 86 tumor tumor NN 87 92 virus virus NN 93 97 with with IN 98 118 activation-dependent activation-dependent JJ 118 119 , , , 120 121 T t NN 122 135 cell-specific cell-specific JJ 136 147 transacting transact VBG 148 155 factors factor NNS 155 156 . . . 158 161 The the DT 162 167 mouse mouse NN 168 175 mammary mammary JJ 176 181 tumor tumor NN 182 187 virus virus NN 188 191 env env NN 192 196 gene gene NN 197 205 contains contain VBZ 206 207 a a DT 208 223 transcriptional transcriptional JJ 224 233 activator activator NN 234 235 ( ( ( 235 239 META META NNP 239 240 ) ) ) 241 245 that that WDT 246 249 can can MD 250 257 control control VB 258 271 transcription transcription NN 272 274 of of IN 275 278 the the DT 279 287 adjacent adjacent JJ 288 292 long long JJ 293 301 terminal terminal JJ 302 308 repeat repeat NN 309 315 region region NN 315 316 . . . 317 332 Transcriptional transcriptional JJ 333 340 control control NN 341 343 by by IN 344 348 META META NNP 349 358 parallels parallel VBZ 359 363 that that DT 364 366 of of IN 367 374 several several JJ 375 385 lymphokine lymphokine NN 386 391 genes gene NNS 391 392 , , , 393 398 being be VBG 399 407 specific specific JJ 408 410 to to TO 411 412 T t NN 413 418 cells cell NNS 418 419 , , , 420 429 dependent dependent JJ 430 432 on on IN 433 438 their their PRP$ 439 449 activation activation NN 449 450 , , , 451 454 and and CC 455 464 inhibited inhibit VBN 465 467 by by IN 468 471 the the DT 472 489 immunosuppressive immunosuppressive JJ 490 494 drug drug NN 495 507 cyclosporine cyclosporine NN 508 509 ( ( ( 509 512 CsA CsA NNP 512 513 ) ) ) 513 514 . . . 515 520 DNase DNase NNP 521 522 I i CD 523 535 footprinting footprinting NN 536 545 indicated indicate VBD 546 550 that that IN 551 558 nuclear nuclear JJ 559 566 factors factor NNS 567 571 from from IN 572 581 activated activate VBN 582 583 T t NN 584 595 lymphocytes lymphocyte NNS 596 601 bound bind VBD 602 603 a a DT 604 621 promoter-proximal promoter-proximal JJ 622 626 site site NN 626 627 , , , 628 635 META(P) meta(p) NN 635 636 , , , 637 640 and and CC 641 642 a a DT 643 658 promoter-distal promoter-distal JJ 659 663 site site NN 663 664 , , , 665 673 META(D+) META(D+) NNP 673 674 , , , 675 681 within within IN 682 685 the the DT 686 694 400-base 400-base JJ 695 699 pair pair NN 700 704 META META NNP 705 711 region region NN 711 712 . . . 713 720 Nuclear nuclear JJ 721 728 factors factor NNS 729 733 from from IN 734 746 unstimulated unstimulated JJ 746 747 , , , 748 751 but but CC 752 755 not not RB 756 760 from from IN 761 770 activated activate VBN 771 776 cells cell NNS 776 777 , , , 778 783 bound bind VBD 784 785 a a DT 786 790 site site NN 790 791 , , , 792 800 META(D-) META(D-) NNP 800 801 , , , 802 810 adjacent adjacent JJ 811 813 to to TO 814 822 META(D+) meta(d+) NN 822 823 . . . 824 832 META(D+) META(D+) NNP 833 841 directed direct VBD 842 855 transcription transcription NN 856 858 of of IN 859 860 a a DT 861 867 linked link VBN 868 878 luciferase luciferase NN 879 883 gene gene NN 883 884 , , , 885 888 and and CC 889 892 gel gel NN 893 898 shift shift NN 899 907 analysis analysis NN 908 916 revealed reveal VBD 917 924 binding binding NN 925 927 of of IN 928 937 inducible inducible JJ 937 938 , , , 939 952 CsA-sensitive csa-sensitive JJ 953 954 T t NN 955 959 cell cell NN 960 967 factors factor NNS 967 968 , , , 969 971 in in IN 972 980 parallel parallel NN 981 985 with with IN 986 998 transfection transfection NN 999 1006 results result NNS 1006 1007 . . . 1008 1017 Authentic authentic JJ 1018 1022 NFAT NFAT NNP 1023 1026 and and CC 1027 1036 NF-kappaB NF-kappaB NNP 1037 1044 targets target NNS 1045 1048 did do VBD 1049 1052 not not RB 1053 1060 compete compete VB 1061 1064 for for IN 1065 1068 the the DT 1069 1077 META(D+) meta(d+) NN 1078 1085 binding binding NN 1086 1095 factor(s) factor(s) NN 1095 1096 . . . 1097 1100 The the DT 1101 1105 SV40 sv40 NN 1106 1110 core core NN 1111 1119 sequence sequence NN 1120 1128 competed compete VBD 1129 1132 for for IN 1133 1141 META(D+) meta(d+) NN 1142 1149 binding binding NN 1150 1157 factors factor NNS 1157 1158 , , , 1159 1162 but but CC 1163 1171 META(D+) META(D+) NNP 1172 1178 failed fail VBD 1179 1181 to to TO 1182 1189 compete compete VB 1190 1193 for for IN 1194 1197 the the DT 1198 1207 complexes complex NNS 1208 1216 obtained obtain VBN 1217 1221 with with IN 1222 1225 the the DT 1226 1230 SV40 sv40 NN 1231 1236 probe probe NN 1236 1237 . . . 1238 1241 Our our PRP$ 1242 1249 results result NNS 1249 1250 , , , 1251 1256 taken take VBN 1257 1265 together together RB 1265 1266 , , , 1267 1275 indicate indicate VBP 1276 1280 that that IN 1281 1289 META(D+) meta(d+) NN 1290 1292 is be VBZ 1293 1294 a a DT 1295 1300 novel novel JJ 1301 1316 transcriptional transcriptional JJ 1317 1325 enhancer enhancer NN 1326 1333 element element NN 1334 1338 that that WDT 1339 1341 is be VBZ 1342 1349 similar similar JJ 1350 1352 in in IN 1353 1356 its its PRP$ 1357 1366 cell-type cell-type JJ 1367 1378 specificity specificity NN 1378 1379 , , , 1380 1390 activation activation NN 1391 1401 dependence dependence NN 1401 1402 , , , 1403 1406 and and CC 1407 1410 CsA CsA NNP 1411 1422 sensitivity sensitivity NN 1423 1425 to to TO 1426 1429 the the DT 1430 1434 NFAT NFAT NNP 1435 1442 element element NN 1442 1443 . . . 1444 1446 It it PRP 1447 1450 may may MD 1451 1453 be be VB 1454 1462 relevant relevant JJ 1463 1465 to to TO 1466 1469 the the DT 1470 1474 role role NN 1475 1477 of of IN 1478 1482 MMTV MMTV NNP 1483 1485 in in IN 1486 1496 expression expression NN 1497 1499 of of IN 1500 1503 Mls Ml NNP 1504 1512 antigens antigen NNS 1513 1515 or or CC 1516 1519 the the DT 1520 1529 induction induction NN 1530 1532 of of IN 1533 1534 T t NN 1535 1539 cell cell NN 1540 1549 lymphomas lymphoma NNS 1549 1550 . . .