0 11 Interleukin Interleukin NNP 12 14 10 10 CD 15 22 induced induce VBD 23 28 c-fos c-fos NN 29 39 expression expression NN 40 42 in in IN 43 48 human human JJ 49 50 B B NNP 51 56 cells cell NNS 57 59 by by IN 60 70 activation activation NN 71 73 of of IN 74 83 divergent divergent JJ 84 91 protein protein NN 92 99 kinases kinase NNS 99 100 . . . 102 107 IL-10 IL-10 NNP 108 110 is be VBZ 111 112 a a DT 113 119 potent potent JJ 120 128 mediator mediator NN 129 131 of of IN 132 137 human human JJ 138 139 B B NNP 140 144 cell cell NN 145 151 growth growth NN 152 155 and and CC 156 162 plasma plasma NN 163 167 cell cell NN 168 177 formation formation NN 177 178 . . . 179 186 However however RB 186 187 , , , 188 194 signal signal NN 195 207 transduction transduction NN 208 210 of of IN 211 216 IL-10 IL-10 NNP 217 219 in in IN 220 221 B B NNP 222 227 cells cell NNS 228 230 is be VBZ 231 237 poorly poorly RB 238 248 understood understand VBN 248 249 . . . 250 252 In in IN 253 257 this this DT 258 263 study study NN 264 267 the the DT 268 274 effect effect NN 275 277 of of IN 278 283 IL-10 il-10 NN 284 286 on on IN 287 290 the the DT 291 301 expression expression NN 302 304 of of IN 305 308 the the DT 309 322 protooncogene protooncogene NN 323 328 c-fos c-fos NN 329 332 was be VBD 333 345 investigated investigate VBN 345 346 , , , 347 354 because because IN 355 358 Fos Fos NNP 359 364 plays play VBZ 365 366 a a DT 367 376 potential potential JJ 377 381 role role NN 382 384 in in IN 385 388 the the DT 389 399 regulation regulation NN 400 402 of of IN 403 404 B B NNP 405 409 cell cell NN 410 423 proliferation proliferation NN 424 427 and and CC 428 443 differentiation differentiation NN 443 444 . . . 445 446 B B NNP 447 452 cells cell NNS 453 457 were be VBD 458 466 purified purify VBN 467 471 from from IN 472 477 buffy buffy JJ 478 482 coat coat NN 483 495 preparations preparation NNS 496 498 of of IN 499 506 healthy healthy JJ 507 512 blood blood NN 513 519 donors donor NNS 520 522 by by IN 523 531 positive positive JJ 532 541 selection selection NN 542 547 using use VBG 548 550 an an DT 551 555 anti anti JJ 556 560 CD20 CD20 NNP 561 571 monoclonal monoclonal JJ 572 580 antibody antibody NN 581 584 and and CC 585 586 a a DT 587 595 MiniMACS MiniMACS NNP 596 606 separation separation NN 607 611 unit unit NN 611 612 . . . 613 614 B B NNP 615 620 cells cell NNS 621 625 were be VBD 626 639 prestimulated prestimulate VBN 640 644 with with IN 645 648 SAC SAC NNP 649 652 for for IN 653 655 48 48 CD 656 659 hrs hr NNS 659 660 . . . 661 665 Then then RB 665 666 , , , 667 672 cells cell NNS 673 677 were be VBD 678 687 incubated incubate VBN 688 692 with with IN 693 699 medium medium NN 700 702 or or CC 703 708 IL-10 il-10 NN 709 710 ( ( ( 710 713 100 100 CD 714 719 ng/ml ng/ml NN 719 720 ) ) ) 721 724 for for IN 725 727 10 10 CD 728 730 to to TO 731 734 120 120 CD 735 739 min. min. NN 740 743 RNA RNA NNP 744 747 was be VBD 748 757 extracted extract VBN 758 760 by by IN 761 778 phenol/chloroform phenol/chloroform NN 779 782 and and CC 783 788 c-fos c-fos NN 789 799 expression expression NN 800 803 was be VBD 804 812 analyzed analyze VBN 813 815 by by IN 816 819 PCR PCR NNP 820 828 assisted assist VBD 829 833 mRNA mrna NN 834 839 assay assay NN 839 840 . . . 841 842 A a DT 843 854 significant significant JJ 855 858 2-4 2-4 CD 859 863 fold fold JJ 864 872 increase increase NN 873 875 of of IN 876 881 c-fos c-fos NN 882 892 expression expression NN 893 896 was be VBD 897 905 observed observe VBN 906 912 within within IN 913 915 30 30 CD 916 919 min min NN 920 922 of of IN 923 934 stimulation stimulation NN 935 939 with with IN 940 945 IL-10 IL-10 NNP 946 947 ( ( ( 947 948 p p NN 949 950 < < SYM 951 955 0.01 0.01 CD 955 956 ) ) ) 956 957 . . . 958 963 After after IN 964 965 2 2 CD 966 969 hrs hr NNS 970 975 c-fos c-fos NN 976 986 expression expression NN 987 995 declined decline VBD 996 998 to to TO 999 1004 basal basal JJ 1005 1011 levels level NNS 1011 1012 . . . 1013 1016 The the DT 1017 1023 effect effect NN 1024 1026 of of IN 1027 1032 IL-10 IL-10 NNP 1033 1036 was be VBD 1037 1051 dose-dependent dose-dependent JJ 1052 1056 with with IN 1057 1058 a a DT 1059 1066 maximum maximum NN 1067 1078 stimulation stimulation NN 1079 1084 using use VBG 1085 1088 100 100 CD 1089 1094 ng/ml ng/ml NN 1095 1097 of of IN 1098 1103 IL-10 IL-10 NNP 1103 1104 . . . 1105 1108 The the DT 1109 1114 IL-10 il-10 NN 1115 1121 effect effect NN 1122 1124 on on IN 1125 1130 c-fos c-fos NN 1131 1141 expression expression NN 1142 1145 was be VBD 1146 1149 not not RB 1150 1157 blocked block VBN 1158 1160 by by IN 1161 1170 polymyxin polymyxin NN 1171 1173 B. B. NNP 1174 1179 Using use VBG 1180 1183 the the DT 1184 1192 tyrosine tyrosine NN 1193 1199 kinase kinase NNP 1200 1209 inhibitor inhibitor NN 1210 1219 genistein genistein NN 1220 1221 ( ( ( 1221 1223 10 10 CD 1224 1230 microM microM NNP 1230 1231 ) ) ) 1232 1233 a a DT 1234 1242 complete complete JJ 1243 1253 inhibition inhibition NN 1254 1256 of of IN 1257 1262 IL-10 IL-10 NNP 1263 1270 induced induced JJ 1271 1276 c-fos c-fos NN 1277 1287 expression expression NN 1288 1291 was be VBD 1292 1300 observed observe VBN 1300 1301 . . . 1302 1304 In in IN 1305 1313 addition addition NN 1313 1314 , , , 1315 1318 H-7 H-7 NNP 1319 1320 ( ( ( 1320 1322 10 10 CD 1323 1329 microM microM NNP 1329 1330 ) ) ) 1330 1331 , , , 1332 1333 a a DT 1334 1342 specific specific JJ 1343 1352 inhibitor inhibitor NN 1353 1355 of of IN 1356 1372 serine/threonine serine/threonine NN 1373 1380 kinases kinase NNS 1380 1381 , , , 1382 1395 significantly significantly RB 1396 1403 blocked block VBD 1404 1409 IL-10 IL-10 NNP 1410 1418 mediated mediated JJ 1419 1424 c-fos c-fos NN 1425 1435 expression expression NN 1436 1437 ( ( ( 1437 1438 p p NN 1439 1440 < < SYM 1441 1445 0.05 0.05 CD 1445 1446 ) ) ) 1446 1447 . . . 1448 1450 In in IN 1451 1461 conclusion conclusion NN 1461 1462 , , , 1463 1468 these these DT 1469 1473 data datum NNS 1474 1478 show show VBP 1479 1483 that that IN 1484 1489 IL-10 il-10 NN 1490 1497 induces induce VBZ 1498 1503 c-fos c-fos NN 1504 1514 expression expression NN 1515 1517 in in IN 1518 1523 human human JJ 1524 1531 B-cells b-cell NNS 1532 1534 by by IN 1535 1545 activation activation NN 1546 1548 of of IN 1549 1557 tyrosine tyrosine NN 1558 1561 and and CC 1562 1578 serine/threonine serine/threonine NN 1579 1586 kinases kinase NNS 1586 1587 . . . 1588 1593 Since since IN 1594 1598 this this DT 1599 1601 is be VBZ 1602 1605 the the DT 1606 1611 first first JJ 1612 1618 report report NN 1619 1621 on on IN 1622 1627 IL-10 IL-10 NNP 1628 1635 induced induce VBD 1636 1642 signal signal NN 1643 1655 transduction transduction NN 1655 1656 , , , 1657 1662 these these DT 1663 1667 data datum NNS 1668 1671 may may MD 1672 1676 help help VB 1677 1679 to to TO 1680 1688 identify identify VB 1689 1692 the the DT 1693 1706 intracellular intracellular JJ 1707 1717 mechanisms mechanism NNS 1718 1720 by by IN 1721 1726 which which WDT 1727 1732 IL-10 IL-10 NNP 1733 1743 stimulates stimulate VBZ 1744 1749 human human JJ 1750 1757 B-cells b-cell NNS 1757 1758 . . .