0 8 Paternal paternal JJ 9 19 expression expression NN 20 22 of of IN 23 26 WT1 wt1 NN 27 29 in in IN 30 35 human human JJ 36 47 fibroblasts fibroblast NNS 48 51 and and CC 52 63 lymphocytes lymphocyte NNS 63 64 . . . 66 69 The the DT 70 75 Wilms Wilms NNPS 75 76 ’ ' POS 77 82 tumor tumor NN 83 93 suppressor suppressor NN 94 98 gene gene NN 99 100 ( ( ( 101 104 WT1 WT1 NNP 105 106 ) ) ) 107 110 was be VBD 111 121 previously previously RB 122 132 identified identify VBN 133 135 as as IN 136 141 being be VBG 142 151 imprinted imprint VBN 151 152 , , , 153 157 with with IN 158 166 frequent frequent JJ 167 175 maternal maternal JJ 176 186 expression expression NN 187 189 in in IN 190 195 human human JJ 196 205 placentae placentae NN 206 209 and and CC 210 215 fetal fetal JJ 216 222 brains brain NNS 222 223 . . . 224 226 We we PRP 227 235 examined examine VBD 236 239 the the DT 240 255 allele-specific allele-specific JJ 256 266 expression expression NN 267 269 of of IN 270 273 WT1 wt1 NN 274 276 in in IN 277 285 cultured culture VBN 286 291 human human JJ 292 303 fibroblasts fibroblast NNS 304 308 from from IN 309 311 15 15 CD 312 323 individuals individual NNS 323 324 . . . 325 330 Seven seven CD 331 333 of of IN 334 336 15 15 CD 337 347 fibroblast fibroblast NN 348 353 lines line NNS 354 358 were be VBD 359 371 heterozygous heterozygous JJ 372 375 for for IN 376 387 polymorphic polymorphic JJ 388 395 alleles allele NNS 395 396 , , , 397 400 and and CC 401 404 the the DT 405 415 expression expression NN 416 424 patterns pattern NNS 425 429 were be VBD 430 438 variable variable JJ 438 439 , , , 440 444 i.e. i.e. FW 444 445 , , , 446 451 equal equal JJ 451 452 , , , 453 460 unequal unequal JJ 461 463 or or CC 464 475 monoallelic monoallelic JJ 476 484 paternal paternal JJ 485 495 expression expression NN 496 498 in in IN 499 504 three three CD 504 505 , , , 506 509 two two CD 510 513 and and CC 514 517 two two CD 518 523 cases case NNS 523 524 , , , 525 537 respectively respectively RB 537 538 . . . 539 548 Exclusive exclusive JJ 549 557 paternal paternal JJ 558 568 expression expression NN 569 571 of of IN 572 575 WT1 WT1 NNP 576 579 was be VBD 580 584 also also RB 585 590 shown show VBN 591 593 in in IN 594 606 non-cultured non-cultured JJ 607 617 peripheral peripheral JJ 618 629 lymphocytes lymphocyte NNS 630 634 from from IN 635 638 the the DT 639 645 latter latter JJ 646 649 two two CD 650 661 individuals individual NNS 661 662 . . . 663 666 The the DT 667 682 allele-specific allele-specific JJ 683 693 expression expression NN 694 702 profiles profile NNS 703 705 of of IN 706 711 other other JJ 712 721 imprinted imprinted JJ 722 727 genes gene NNS 727 728 , , , 729 733 IGF2 igf2 NN 734 737 and and CC 738 741 H19 h19 NN 741 742 , , , 743 745 on on IN 746 751 human human JJ 752 762 chromosome chromosome NN 763 765 11 11 CD 766 770 were be VBD 771 779 constant constant JJ 780 783 and and CC 784 794 consistent consistent JJ 795 799 with with IN 800 805 those those DT 806 808 in in IN 809 814 other other JJ 815 822 tissues tissue NNS 822 823 . . . 824 827 Our our PRP$ 828 838 unexpected unexpected JJ 839 851 observations observation NNS 852 854 of of IN 855 863 paternal paternal JJ 864 866 or or CC 867 876 biallelic biallelic JJ 877 887 expression expression NN 888 890 of of IN 891 894 WT1 wt1 NN 895 897 in in IN 898 909 fibroblasts fibroblast NNS 910 913 and and CC 914 925 lymphocytes lymphocyte NNS 925 926 , , , 927 935 together together RB 936 940 with with IN 941 944 the the DT 945 953 previous previous JJ 954 962 findings finding NNS 963 965 of of IN 966 974 maternal maternal JJ 975 977 or or CC 978 987 biallelic biallelic JJ 988 998 expression expression NN 999 1001 in in IN 1002 1011 placentae placenta NNS 1012 1015 and and CC 1016 1022 brains brain NNS 1022 1023 , , , 1024 1031 suggest suggest VBP 1032 1036 that that IN 1037 1040 the the DT 1041 1056 allele-specific allele-specific JJ 1057 1067 regulatory regulatory JJ 1068 1074 system system NN 1075 1077 of of IN 1078 1081 WT1 WT1 NNP 1082 1084 is be VBZ 1085 1091 unique unique JJ 1092 1095 and and CC 1096 1099 may may MD 1100 1102 be be VB 1103 1113 controlled control VBN 1114 1116 by by IN 1117 1118 a a DT 1119 1127 putative putative JJ 1128 1135 tissue- tissue- NN 1136 1139 and and CC 1140 1159 individual-specific individual-specific JJ 1160 1168 modifier modifier NN 1168 1169 . . .