0 10 Regulation regulation NN 11 13 of of IN 14 31 interleukin-1beta interleukin-1beta NN 32 45 transcription transcription NN 46 48 by by IN 49 61 Epstein-Barr epstein-barr JJ 62 67 virus virus NN 68 76 involves involve VBZ 77 78 a a DT 79 85 number number NN 86 88 of of IN 89 95 latent latent JJ 96 104 proteins protein NNS 105 108 via via IN 109 114 their their PRP$ 115 126 interaction interaction NN 127 131 with with IN 132 135 RBP RBP NNP 135 136 . . . 138 150 Epstein-Barr epstein-barr JJ 151 156 virus virus NN 157 158 ( ( ( 158 161 EBV EBV NNP 161 162 ) ) ) 163 170 infects infect VBZ 171 172 B B NNP 173 178 cells cell NNS 178 179 , , , 180 189 resulting result VBG 190 192 in in IN 193 196 the the DT 197 206 outgrowth outgrowth NN 207 209 of of IN 210 222 immortalised immortalised JJ 223 237 lymphoblastoid lymphoblastoid JJ 238 242 cell cell NN 243 248 lines line NNS 249 250 ( ( ( 250 254 LCLs LCL NNP 254 255 ) ) ) 255 256 . . . 257 261 Here here RB 261 262 , , , 263 265 we we PRP 266 277 demonstrate demonstrate VBP 278 285 through through IN 286 289 the the DT 290 293 use use NN 294 296 of of IN 297 310 intracellular intracellular JJ 311 319 staining staining NN 320 324 that that IN 325 342 interleukin-1beta interleukin-1beta NN 343 344 ( ( ( 344 352 IL-1beta IL-1beta NNP 352 353 ) ) ) 354 356 is be VBZ 357 366 expressed express VBN 367 369 in in IN 370 374 LCLs lcl NNS 375 378 and and CC 379 390 investigate investigate VB 391 394 the the DT 395 404 influence influence NN 405 407 of of IN 408 411 the the DT 412 422 individual individual JJ 423 429 latent latent JJ 430 438 proteins protein NNS 439 441 on on IN 442 445 the the DT 446 456 expression expression NN 457 459 of of IN 460 468 IL-1beta IL-1beta NNP 468 469 . . . 470 475 Using use VBG 476 482 RT-PCR RT-PCR NNP 482 483 , , , 484 492 IL-1beta IL-1beta NNP 493 496 was be VBD 497 502 shown show VBN 503 505 to to TO 506 508 be be VB 509 521 up-regulated up-regulate VBN 522 524 in in IN 525 540 EBV-transformed ebv-transformed JJ 541 545 LCLs LCL NNP 546 548 as as RB 549 553 well well RB 554 556 as as IN 557 559 in in IN 560 565 group group NN 566 569 III iii CD 570 577 Burkitt Burkitt NNP 577 579 ’s 's POS 580 588 lymphoma lymphoma NN 589 590 ( ( ( 590 592 BL BL NNP 592 593 ) ) ) 594 598 cell cell NN 599 604 lines line NNS 604 605 , , , 606 614 compared compare VBN 615 619 with with IN 620 625 group group NN 626 627 I i CD 628 630 BL bl NN 631 635 cell cell NN 636 641 lines line NNS 641 642 . . . 643 646 The the DT 647 660 up-regulation up-regulation NN 661 663 of of IN 664 672 IL-1beta IL-1beta NNP 673 680 message message NN 681 686 could could MD 687 689 be be VB 690 698 mediated mediate VBN 699 701 by by IN 702 705 the the DT 706 712 latent latent JJ 713 721 membrane membrane NN 722 731 protein-1 protein-1 NN 731 732 , , , 733 736 EBV EBV NNP 737 744 nuclear nuclear JJ 745 753 proteins protein NNS 754 755 2 2 CD 755 756 , , , 757 758 3 3 CD 758 759 , , , 760 761 4 4 CD 761 762 , , , 763 766 and and CC 767 768 6 6 CD 769 774 genes gene NNS 774 775 . . . 776 791 Electrophoretic electrophoretic JJ 792 800 mobility mobility NN 801 806 shift shift NN 807 813 assays assay NNS 814 815 ( ( ( 815 820 EMSAs EMSA NNP 820 821 ) ) ) 822 834 demonstrated demonstrate VBD 835 839 that that IN 840 843 the the DT 844 848 -300 -300 CD 849 855 region region NN 856 858 of of IN 859 862 the the DT 863 871 IL-1beta IL-1beta NNP 872 880 promoter promoter NN 880 881 , , , 882 887 which which WDT 888 896 contains contain VBZ 897 898 a a DT 899 906 nuclear nuclear JJ 907 920 factor-kappaB factor-kappab NN 921 922 ( ( ( 922 931 NF-kappaB NF-kappaB NNP 931 932 ) ) ) 933 940 binding binding NN 941 945 site site NN 945 946 , , , 947 956 contained contain VBD 957 958 a a DT 959 969 functional functional JJ 970 973 RBP RBP NNP 974 981 binding binding NN 982 986 site site NN 986 987 . . . 988 995 Binding binding NN 996 998 of of IN 999 1002 RBP RBP NNP 1003 1005 to to TO 1006 1010 this this DT 1011 1015 site site NN 1016 1021 could could MD 1022 1024 be be VB 1025 1034 inhibited inhibit VBN 1035 1037 by by IN 1038 1046 addition addition NN 1047 1049 of of IN 1050 1053 EBV EBV NNP 1054 1061 nuclear nuclear JJ 1062 1070 proteins protein NNS 1071 1072 3 3 CD 1073 1076 and and CC 1077 1078 6 6 CD 1078 1079 , , , 1080 1090 suggesting suggest VBG 1091 1095 that that IN 1096 1101 these these DT 1102 1110 proteins protein NNS 1111 1119 displace displace VBP 1120 1123 RBP RBP NNP 1124 1128 from from IN 1129 1132 its its PRP$ 1133 1144 recognition recognition NN 1145 1153 sequence sequence NN 1153 1154 , , , 1155 1163 removing remove VBG 1164 1179 transcriptional transcriptional JJ 1180 1190 repression repression NN 1191 1194 and and CC 1195 1203 allowing allow VBG 1204 1208 gene gene NN 1209 1222 transcription transcription NN 1223 1225 to to TO 1226 1231 occur occur VB 1231 1232 . . . 1233 1235 In in IN 1236 1241 group group NN 1242 1243 I i CD 1244 1246 BL BL NNP 1247 1252 cells cell NNS 1252 1253 , , , 1254 1264 containing contain VBG 1265 1268 low low JJ 1269 1275 levels level NNS 1276 1278 of of IN 1279 1288 NF-kappaB NF-kappaB NNP 1288 1289 , , , 1290 1294 only only RB 1295 1298 RBP RBP NNP 1299 1306 binding binding NN 1307 1310 was be VBD 1311 1319 observed observe VBN 1320 1322 in in IN 1323 1328 EMSAs EMSA NNP 1328 1329 , , , 1330 1337 whereas whereas IN 1338 1347 NF-kappaB NF-kappaB NNP 1348 1355 binding binding NN 1356 1361 could could MD 1362 1364 be be VB 1365 1377 demonstrated demonstrate VBN 1378 1380 in in IN 1381 1396 EBV-transformed ebv-transformed JJ 1397 1398 B B NNP 1399 1403 cell cell NN 1404 1409 lines line NNS 1410 1420 containing contain VBG 1421 1425 high high JJ 1426 1432 levels level NNS 1433 1435 of of IN 1436 1445 activated activate VBN 1446 1455 NF-kappaB nf-kappab NN 1455 1456 . . . 1457 1459 In in IN 1460 1468 addition addition NN 1468 1469 , , , 1470 1473 the the DT 1474 1484 expression expression NN 1485 1487 of of IN 1488 1494 latent latent JJ 1495 1503 membrane membrane NN 1504 1513 protein-1 protein-1 NN 1514 1517 led lead VBD 1518 1520 to to TO 1521 1531 activation activation NN 1532 1534 of of IN 1535 1544 NF-kappaB NF-kappaB NNP 1545 1549 that that WDT 1550 1553 was be VBD 1554 1561 capable capable JJ 1562 1564 of of IN 1565 1572 binding bind VBG 1573 1576 the the DT 1577 1585 IL-1beta IL-1beta NNP 1586 1594 promoter promoter NN 1594 1595 . . . 1596 1599 The the DT 1600 1605 study study NN 1606 1618 demonstrates demonstrate VBZ 1619 1623 that that IN 1624 1627 EBV EBV NNP 1628 1631 can can MD 1632 1643 up-regulate up-regulate VB 1644 1652 IL-1beta IL-1beta NNP 1653 1663 expression expression NN 1663 1664 , , , 1665 1673 possibly possibly RB 1674 1676 by by IN 1677 1682 using use VBG 1683 1686 RBP RBP NNP 1686 1687 , , , 1688 1697 NF-kappaB NF-kappaB NNP 1697 1698 , , , 1699 1701 or or CC 1702 1706 both both DT 1706 1707 . . . 1708 1717 Copyright Copyright NNP 1718 1722 1998 1998 CD 1723 1731 Academic Academic NNP 1732 1737 Press Press NNP 1737 1738 . . .