0 16 Characterization characterization NN 17 19 of of IN 20 23 the the DT 24 29 human human JJ 30 35 elk-1 elk-1 NN 36 44 promoter promoter NN 44 45 . . . 46 55 Potential potential JJ 56 60 role role NN 61 63 of of IN 64 65 a a DT 66 76 downstream downstream JJ 77 85 intronic intronic JJ 86 94 sequence sequence NN 95 98 for for IN 99 104 elk-1 elk-1 NN 105 109 gene gene NN 110 120 expression expression NN 121 123 in in IN 124 133 monocytes monocyte NNS 133 134 . . . 136 138 To to TO 139 151 characterize characterize VB 152 155 the the DT 156 161 human human JJ 162 167 elk-1 elk-1 NN 168 176 promoter promoter NN 176 177 , , , 178 180 we we PRP 181 187 mapped map VBD 188 191 the the DT 192 207 transcriptional transcriptional JJ 208 213 start start NN 214 218 site site NN 219 222 and and CC 223 231 isolated isolate VBN 232 246 elk-1-specific elk-1-specific JJ 247 254 genomic genomic JJ 255 260 phage phage NN 261 267 clones clone NNS 268 272 that that WDT 273 282 contained contain VBD 283 292 extensive extensive JJ 293 301 upstream upstream JJ 302 305 and and CC 306 316 downstream downstream JJ 317 326 sequences sequence NNS 326 327 . . . 328 329 A a DT 330 339 TATA-like tata-like JJ 340 345 motif motif NN 346 349 was be VBD 350 360 identified identify VBN 361 372 immediately immediately RB 373 381 upstream upstream RB 382 384 of of IN 385 388 the the DT 389 404 transcriptional transcriptional JJ 405 410 start start NN 411 415 site site NN 415 416 . . . 417 427 Functional functional JJ 428 436 analyses analysis NNS 437 439 of of IN 440 443 DNA DNA NNP 444 453 fragments fragment NNS 454 464 containing contain VBG 465 468 the the DT 469 473 TATA TATA NNP 474 481 element element NN 482 485 and and CC 486 489 the the DT 490 504 identification identification NN 505 507 of of IN 508 509 a a DT 510 515 DNase DNase NNP 516 532 I-hypersensitive i-hypersensitive JJ 533 542 chromatin chromatin NN 543 547 site site NN 548 549 ( ( ( 549 551 HS HS NNP 552 553 1 1 CD 553 554 ) ) ) 555 557 in in IN 558 563 close close JJ 564 573 proximity proximity NN 574 576 to to TO 577 580 the the DT 581 585 TATA TATA NNP 586 589 box box NN 590 597 suggest suggest VBP 598 602 that that IN 603 606 the the DT 607 617 identified identify VBN 618 622 TATA TATA NNP 623 628 motif motif NN 629 631 is be VBZ 632 641 important important JJ 642 645 for for IN 646 651 elk-1 elk-1 NN 652 665 transcription transcription NN 666 668 in in FW 669 673 vivo vivo FW 673 674 . . . 675 684 Sequences Sequence NNP 685 693 upstream upstream RB 694 697 and and CC 698 708 downstream downstream RB 709 713 from from IN 714 717 the the DT 718 722 TATA TATA NNP 723 726 box box NN 727 731 were be VBD 732 737 found find VBN 738 740 to to TO 741 751 contribute contribute VB 752 754 to to TO 755 760 elk-1 elk-1 NN 761 769 promoter promoter NN 770 778 activity activity NN 778 779 . . . 780 781 A a DT 782 788 second second JJ 789 803 hypersensitive hypersensitive JJ 804 808 site site NN 809 810 ( ( ( 810 812 HS HS NNP 813 814 2 2 CD 814 815 ) ) ) 816 819 was be VBD 820 830 identified identify VBN 831 837 within within IN 838 841 the the DT 842 847 first first JJ 848 854 intron intron NN 855 857 in in IN 858 871 pre-monocytic pre-monocytic JJ 872 877 cells cell NNS 877 878 , , , 879 884 which which WDT 885 892 express express VBP 893 898 Elk-1 elk-1 NN 899 903 only only RB 904 908 when when WRB 909 924 differentiating differentiate VBG 925 927 to to TO 928 937 monocytes monocyte NNS 937 938 . . . 939 941 In in IN 942 943 a a DT 944 951 variety variety NN 952 954 of of IN 955 960 other other JJ 961 965 cell cell NN 966 971 types type NNS 971 972 , , , 973 978 which which WDT 979 986 display display VBP 987 988 a a DT 989 1001 constitutive constitutive JJ 1002 1007 Elk-1 elk-1 NN 1008 1018 expression expression NN 1018 1019 , , , 1020 1022 HS HS NNP 1023 1024 2 2 CD 1025 1028 did do VBD 1029 1032 not not RB 1033 1038 exist exist VB 1038 1039 , , , 1040 1050 suggesting suggest VBG 1051 1055 that that IN 1056 1068 inducibility inducibility NN 1069 1071 of of IN 1072 1077 elk-1 elk-1 NN 1078 1088 expression expression NN 1089 1091 is be VBZ 1092 1102 associated associate VBN 1103 1107 with with IN 1108 1111 the the DT 1112 1120 presence presence NN 1121 1123 of of IN 1124 1126 HS HS NNP 1127 1128 2 2 CD 1128 1129 . . . 1130 1135 Egr-1 Egr-1 NNP 1136 1139 and and CC 1140 1143 the the DT 1144 1149 serum serum NN 1150 1158 response response NN 1159 1165 factor factor NN 1166 1170 were be VBD 1171 1176 found find VBN 1177 1179 to to TO 1180 1188 interact interact VB 1189 1201 specifically specifically RB 1202 1206 with with IN 1207 1210 the the DT 1211 1219 intronic intronic JJ 1220 1228 sequence sequence NN 1229 1231 at at IN 1232 1236 +265 +265 CD 1237 1240 and and CC 1241 1245 +448 +448 CD 1245 1246 , , , 1247 1259 respectively respectively RB 1259 1260 . . . 1261 1268 Because because IN 1269 1274 Egr-1 egr-1 NN 1275 1279 mRNA mRNA NNP 1280 1283 and and CC 1284 1291 protein protein NN 1292 1298 levels level NNS 1299 1303 were be VBD 1304 1312 observed observe VBN 1313 1315 to to TO 1316 1324 increase increase VB 1325 1338 significantly significantly RB 1339 1345 before before IN 1346 1355 induction induction NN 1356 1358 of of IN 1359 1364 elk-1 elk-1 NN 1365 1375 expression expression NN 1375 1376 , , , 1377 1379 we we PRP 1380 1387 propose propose VBP 1388 1392 that that IN 1393 1398 Egr-1 egr-1 NN 1399 1401 is be VBZ 1402 1411 important important JJ 1412 1415 for for IN 1416 1419 the the DT 1420 1430 regulation regulation NN 1431 1433 of of IN 1434 1439 elk-1 elk-1 NN 1440 1453 transcription transcription NN 1454 1456 in in IN 1457 1472 differentiating differentiate VBG 1473 1482 monocytes monocyte NNS 1482 1483 . . .