0 16 Lineage-specific lineage-specific JJ 17 27 activation activation NN 28 30 of of IN 31 36 STAT3 stat3 NN 37 39 by by IN 40 56 interferon-gamma interferon-gamma NN 57 59 in in IN 60 65 human human JJ 66 77 neutrophils neutrophil NNS 77 78 . . . 80 87 Binding binding NN 88 90 of of IN 91 107 interferon-gamma interferon-gamma NN 108 109 ( ( ( 109 118 IFN-gamma IFN-gamma NNP 118 119 ) ) ) 120 122 to to TO 123 126 its its PRP$ 127 140 heterodimeric heterodimeric JJ 141 149 receptor receptor NN 150 157 induces induce VBZ 158 168 activation activation NN 169 171 of of IN 172 175 the the DT 176 184 tyrosine tyrosine NN 185 192 kinases kinase NNS 193 197 JAK1 jak1 NN 198 201 and and CC 202 206 JAK2 jak2 NN 207 215 followed follow VBN 216 218 by by IN 219 227 tyrosine tyrosine NN 228 243 phosphorylation phosphorylation NN 244 246 of of IN 247 257 STAT1alpha STAT1alpha NNP 257 258 . . . 259 268 Selective selective JJ 269 279 activation activation NN 280 282 of of IN 283 293 STAT1alpha STAT1alpha NNP 294 296 at at IN 297 300 the the DT 301 310 IFN-gamma IFN-gamma NNP 311 319 receptor receptor NN 320 322 is be VBZ 323 331 achieved achieve VBN 332 334 by by IN 335 343 specific specific JJ 344 355 interaction interaction NN 356 363 between between IN 364 365 a a DT 366 375 cytosolic cytosolic JJ 376 384 tyrosine tyrosine NN 385 390 motif motif NN 391 400 including include VBG 401 405 Y440 y440 NN 406 408 in in IN 409 412 the the DT 413 422 IFN-gamma IFN-gamma NNP 423 431 receptor receptor NN 432 443 alpha-chain alpha-chain NN 444 447 and and CC 448 451 the the DT 452 455 SH2 sh2 NN 456 462 domain domain NN 463 465 of of IN 466 476 STAT1alpha STAT1alpha NNP 476 477 . . . 478 480 We we PRP 481 492 demonstrate demonstrate VBP 493 497 that that IN 497 498 , , , 499 501 in in IN 502 510 addition addition NN 511 513 to to TO 514 524 STAT1alpha STAT1alpha NNP 524 525 , , , 526 531 STAT3 STAT3 NNP 532 534 is be VBZ 535 539 also also RB 540 549 activated activate VBN 550 552 by by IN 553 562 IFN-gamma IFN-gamma NNP 563 565 in in IN 566 571 human human JJ 572 583 neutrophils neutrophil NNS 583 584 . . . 585 588 The the DT 589 599 activation activation NN 600 602 of of IN 603 608 STAT3 STAT3 NNP 609 612 was be VBD 613 616 not not RB 617 622 found find VBN 623 625 in in IN 626 631 human human JJ 632 643 eosinophils eosinophil NNS 643 644 , , , 645 654 monocytes monocyte NNS 654 655 , , , 656 659 and and CC 660 665 HL-60 hl-60 NN 666 671 cells cell NNS 671 672 , , , 673 681 although although IN 682 685 the the DT 686 691 STAT3 stat3 NN 692 699 protein protein NN 700 703 was be VBD 704 713 expressed express VBN 714 716 in in IN 717 722 these these DT 723 728 cells cell NNS 728 729 . . . 730 733 The the DT 734 738 cell cell NN 739 752 type-specific type-specific JJ 753 763 activation activation NN 764 766 of of IN 767 772 STAT3 STAT3 NNP 773 775 by by IN 776 785 IFN-gamma IFN-gamma NNP 786 789 was be VBD 790 794 also also RB 795 803 observed observe VBN 804 806 in in IN 807 818 neutrophils neutrophil NNS 819 823 that that WDT 824 827 are be VBP 828 842 differentiated differentiate VBN 843 845 in in FW 846 851 vitro vitro FW 852 856 from from IN 857 862 human human JJ 863 868 CD34+ cd34+ JJ 869 882 hematopoietic hematopoietic JJ 883 887 stem stem NN 888 893 cells cell NNS 893 894 . . . 895 900 These these DT 901 908 results result NNS 909 917 indicate indicate VBP 918 922 that that IN 923 924 a a DT 925 931 single single JJ 932 940 cytokine cytokine NN 941 949 receptor receptor NN 950 953 can can MD 954 962 activate activate VB 963 972 different different JJ 973 977 STAT STAT NNP 978 984 family family NN 985 992 members member NNS 993 995 in in IN 996 997 a a DT 998 1011 cell-specific cell-specific JJ 1012 1018 manner manner NN 1018 1019 , , , 1020 1025 which which WDT 1026 1031 might might MD 1032 1038 result result VB 1039 1041 in in IN 1042 1055 cell-specific cell-specific JJ 1056 1060 gene gene NN 1061 1074 transcription transcription NN 1074 1075 . . .