0 5 Spi-C Spi-C NNP 5 6 , , , 7 8 a a DT 9 14 novel novel JJ 15 18 Ets ets NN 19 26 protein protein NN 27 31 that that WDT 32 34 is be VBZ 35 45 temporally temporally RB 46 55 regulated regulate VBN 56 62 during during IN 63 64 B B NNP 65 75 lymphocyte lymphocyte NN 76 87 development development NN 87 88 . . . 90 91 A a DT 92 97 novel novel JJ 98 101 Ets ets NN 102 109 protein protein NN 110 113 was be VBD 114 122 isolated isolate VBN 123 125 by by IN 126 131 yeast yeast NN 132 142 one-hybrid one-hybrid JJ 143 152 screening screening NN 153 155 of of IN 156 157 a a DT 158 162 cDNA cdna NN 163 170 library library NN 171 175 made make VBN 176 180 from from IN 181 210 lipopolysaccharide-stimulated lipopolysaccharide-stimulated JJ 211 216 mouse mouse NN 217 224 splenic splenic JJ 225 226 B B NNP 227 232 cells cell NNS 232 233 , , , 234 239 using use VBG 240 243 the the DT 244 247 SP6 SP6 NNP 248 253 kappa kappa NN 254 262 promoter promoter NN 263 269 kappaY kappay NN 270 277 element element NN 278 280 as as IN 281 282 a a DT 283 287 bait bait NN 287 288 . . . 289 292 The the DT 293 298 novel novel JJ 299 302 Ets ets NN 303 310 protein protein NN 311 314 was be VBD 315 319 most most RBS 320 327 closely closely RB 328 335 related related JJ 336 338 to to TO 339 343 PU.1 PU.1 NNP 344 347 and and CC 348 353 Spi-B Spi-B NNP 354 360 within within IN 361 364 the the DT 365 368 DNA dna NN 369 376 binding binding NN 377 380 Ets Ets NNP 381 387 domain domain NN 388 391 and and CC 392 395 was be VBD 396 405 therefore therefore RB 406 411 named name VBN 412 417 Spi-C Spi-C NNP 417 418 . . . 419 426 However however RB 426 427 , , , 428 433 Spi-C Spi-C NNP 434 437 may may MD 438 447 represent represent VB 448 449 a a DT 450 455 novel novel JJ 456 464 subgroup subgroup NN 465 471 within within IN 472 475 the the DT 476 479 Ets Ets NNP 480 487 protein protein NN 488 494 family family NN 494 495 , , , 496 498 as as IN 499 501 it it PRP 502 510 differed differ VBD 511 524 significantly significantly RB 525 529 from from IN 530 535 Spi-B Spi-B NNP 536 539 and and CC 540 544 PU.1 pu.1 NN 545 551 within within IN 552 557 helix helix NN 558 559 1 1 CD 560 562 of of IN 563 566 the the DT 567 570 Ets Ets NNP 571 577 domain domain NN 577 578 . . . 579 584 Spi-C Spi-C NNP 585 588 was be VBD 589 596 encoded encode VBN 597 599 by by IN 600 601 a a DT 602 613 single-copy single-copy JJ 614 618 gene gene NN 619 623 that that WDT 624 627 was be VBD 628 634 mapped map VBN 635 637 to to TO 638 648 chromosome chromosome NN 649 651 10 10 CD 651 652 , , , 653 659 region region NN 660 662 C. C. NNP 663 668 Spi-C Spi-C NNP 669 679 interacted interact VBD 680 684 with with IN 685 688 DNA DNA NNP 689 698 similarly similarly RB 699 701 to to TO 702 706 PU.1 pu.1 NN 707 709 as as IN 710 716 judged judge VBN 717 719 by by IN 720 731 methylation methylation NN 732 744 interference interference NN 744 745 , , , 746 756 band-shift band-shift NN 757 760 and and CC 761 765 site site NN 766 775 selection selection NN 776 784 analysis analysis NN 784 785 , , , 786 789 and and CC 790 799 activated activate VBD 800 813 transcription transcription NN 814 816 of of IN 817 818 a a DT 819 825 kappaY kappay NN 826 833 element element NN 834 842 reporter reporter NN 843 847 gene gene NN 848 852 upon upon IN 853 868 co-transfection co-transfection NN 869 871 of of IN 872 876 HeLa HeLa NNP 877 882 cells cell NNS 882 883 . . . 884 889 Spi-C Spi-C NNP 890 893 RNA RNA NNP 894 897 was be VBD 898 907 expressed express VBN 908 910 in in IN 911 917 mature mature JJ 918 919 B B NNP 920 931 lymphocytes lymphocyte NNS 932 935 and and CC 936 938 at at IN 939 944 lower low JJR 945 951 levels level NNS 952 954 in in IN 955 966 macrophages macrophage NNS 966 967 . . . 968 979 Furthermore furthermore RB 979 980 , , , 981 986 pre-B pre-b JJ 987 991 cell cell NN 992 995 and and CC 996 1002 plasma plasma NN 1003 1007 cell cell NN 1008 1013 lines line NNS 1014 1018 were be VBD 1019 1033 Spi-C-negative spi-c-negative JJ 1033 1034 , , , 1035 1045 suggesting suggest VBG 1046 1050 that that IN 1051 1056 Spi-C Spi-C NNP 1057 1062 might might MD 1063 1065 be be VB 1066 1067 a a DT 1068 1078 regulatory regulatory JJ 1079 1087 molecule molecule NN 1088 1094 during during IN 1095 1096 a a DT 1097 1105 specific specific JJ 1106 1111 phase phase NN 1112 1114 of of IN 1115 1116 B B NNP 1117 1125 lymphoid lymphoid JJ 1126 1137 development development NN 1137 1138 . . .