0 6 Signal Signal NNP 7 19 transduction transduction NN 20 27 through through IN 28 44 interferon-gamma interferon-gamma NN 45 53 receptor receptor NN 54 56 on on IN 57 62 human human JJ 63 74 eosinophils eosinophil NNS 74 75 . . . 77 87 BACKGROUND background NN 87 88 : : : 89 91 We we PRP 92 100 reported report VBD 101 103 on on IN 104 107 the the DT 108 120 constitutive constitutive JJ 121 137 interferon-gamma interferon-gamma NN 138 146 receptor receptor NN 147 148 ( ( ( 148 158 IFN-gammaR IFN-gammaR NNP 158 159 ) ) ) 160 170 expression expression NN 171 173 on on IN 174 185 eosinophils eosinophil NNS 185 186 . . . 187 190 But but CC 191 197 signal signal NN 198 210 transduction transduction NN 211 218 through through IN 219 229 IFN-gammaR ifn-gammar NN 230 232 on on IN 233 244 eosinophils eosinophil NNS 245 252 remains remain VBZ 253 255 to to TO 256 258 be be VB 259 269 elucidated elucidate VBN 269 270 . . . 271 273 In in IN 274 278 this this DT 279 284 study study NN 284 285 , , , 286 288 we we PRP 289 297 examined examine VBD 298 301 the the DT 302 313 involvement involvement NN 314 316 of of IN 317 320 the the DT 321 329 Jak/Stat Jak/Stat NNP 330 337 pathway pathway NN 338 340 in in IN 341 344 the the DT 345 354 signaling signaling NN 355 357 of of IN 358 369 eosinophils eosinophil NNS 370 375 after after IN 376 386 IFN-gammaR IFN-gammaR NNP 387 398 conjugation conjugation NN 399 401 by by IN 402 405 the the DT 406 412 ligand ligand NN 413 420 binding binding NN 420 421 . . . 422 429 METHODS methods NNS 429 430 : : : 431 439 Purified purify VBN 440 450 peripheral peripheral JJ 451 462 eosinophils eosinophil NNS 463 467 were be VBD 468 478 stimulated stimulate VBN 479 483 with with IN 484 493 IFN-gamma IFN-gamma NNP 494 496 at at IN 497 499 37 37 CD 500 507 degrees degree NNS 508 509 C c JJ 510 513 for for IN 514 518 1-60 1-60 CD 519 523 min. min. NN 524 532 Tyrosine Tyrosine NNP 533 548 phosphorylation phosphorylation NN 549 551 of of IN 552 562 IFN-gammaR IFN-gammaR NNP 562 563 , , , 564 568 Jak1 Jak1 NNP 568 569 , , , 570 574 Jak2 Jak2 NNP 574 575 , , , 576 579 and and CC 580 590 Stat1alpha Stat1alpha NNP 591 594 was be VBD 595 603 examined examine VBN 604 606 by by IN 607 621 immunoblotting immunoblotting NN 621 622 . . . 623 632 Gel-shift gel-shift JJ 633 638 assay assay NN 639 642 was be VBD 643 647 also also RB 648 656 examined examine VBN 657 659 to to TO 660 664 show show VB 665 668 the the DT 669 678 formation formation NN 679 681 of of IN 682 696 Stat1alpha-DNA stat1alpha-dna JJ 697 706 complexes complex NNS 706 707 . . . 708 715 RESULTS results NNS 715 716 : : : 717 719 We we PRP 720 724 show show VBP 725 729 that that IN 730 737 binding binding NN 738 740 of of IN 741 750 IFN-gamma IFN-gamma NNP 751 753 to to TO 754 759 human human JJ 760 771 eosinophils eosinophil NNS 772 781 initiated initiate VBD 782 783 a a DT 784 790 series series NN 791 793 of of IN 794 800 events event NNS 801 805 that that WDT 806 814 resulted result VBD 815 817 in in IN 818 821 the the DT 822 827 rapid rapid JJ 828 836 tyrosine tyrosine NN 837 852 phosphorylation phosphorylation NN 853 855 of of IN 856 859 not not RB 860 864 only only RB 865 868 the the DT 869 884 IFN-gammaRalpha IFN-gammaRalpha NNP 885 890 chain chain NN 891 894 but but CC 895 899 also also RB 900 904 Jak1 jak1 NN 904 905 , , , 906 910 Jak2 Jak2 NNP 910 911 , , , 912 915 and and CC 916 926 Stat1alpha Stat1alpha NNP 926 927 . . . 928 930 In in IN 931 939 addition addition NN 939 940 , , , 941 950 IFN-gamma IFN-gamma NNP 951 959 enhanced enhance VBD 960 963 the the DT 964 975 DNA-binding dna-binding JJ 976 984 activity activity NN 985 987 of of IN 988 998 Stat1alpha Stat1alpha NNP 998 999 . . . 1000 1010 CONCLUSION conclusion NN 1010 1011 : : : 1012 1017 These these DT 1018 1022 data datum NNS 1023 1031 indicate indicate VBP 1032 1036 that that IN 1037 1046 IFN-gamma IFN-gamma NNP 1047 1054 affects affect VBZ 1055 1066 eosinophils eosinophil NNS 1067 1074 through through IN 1075 1078 its its PRP$ 1079 1087 specific specific JJ 1088 1096 receptor receptor NN 1097 1100 and and CC 1101 1109 utilizes utilize VBZ 1110 1113 the the DT 1114 1122 Jak/Stat Jak/Stat NNP 1123 1130 pathway pathway NN 1131 1133 as as IN 1134 1137 its its PRP$ 1138 1142 mode mode NN 1143 1145 of of IN 1146 1155 signaling signaling NN 1155 1156 . . .