0 7 Nuclear nuclear JJ 8 17 factor-90 factor-90 NN 18 20 of of IN 21 30 activated activate VBN 31 38 T-cells t-cell NNS 38 39 : : : 40 41 A a DT 42 57 double-stranded double-stranded JJ 58 69 RNA-binding rna-binding JJ 70 77 protein protein NN 78 81 and and CC 82 91 substrate substrate NN 92 95 for for IN 96 99 the the DT 100 115 double-stranded double-stranded JJ 116 129 RNA-dependent rna-dependent JJ 130 137 protein protein NN 138 144 kinase kinase NNP 144 145 , , , 146 149 PKR PKR NNP 149 150 . . . 152 156 NFAT NFAT NNP 157 170 transcription transcription NN 171 178 factors factor NNS 179 183 play play VBP 184 185 a a DT 186 193 central central JJ 194 198 role role NN 199 201 in in IN 202 212 initiating initiate VBG 213 219 T-cell t-cell NN 220 230 activation activation NN 231 238 through through IN 239 242 the the DT 243 252 induction induction NN 253 255 of of IN 256 271 immediate-early immediate-early JJ 272 278 T-cell t-cell NN 279 287 specific specific JJ 288 293 genes gene NNS 294 303 including include VBG 304 317 interleukin-2 interleukin-2 NN 318 319 ( ( ( 319 323 IL-2 il-2 NN 323 324 ) ) ) 324 325 . . . 326 330 NFAT NFAT NNP 331 344 transcription transcription NN 345 352 factors factor NNS 353 357 bind bind VBP 358 360 to to TO 361 362 a a DT 363 371 sequence sequence NN 372 374 in in IN 375 378 the the DT 379 383 IL-2 il-2 NN 384 392 enhancer enhancer NN 393 398 known know VBN 399 401 as as IN 402 405 the the DT 406 413 antigen antigen NN 414 422 receptor receptor NN 423 431 response response NN 432 439 element element NN 440 441 2 2 CD 442 443 ( ( ( 443 449 ARRE-2 arre-2 NN 449 450 ) ) ) 450 451 . . . 452 460 Multiple multiple JJ 461 469 proteins protein NNS 470 480 exhibiting exhibit VBG 481 487 ARRE-2 arre-2 NN 488 495 binding binding NN 496 504 activity activity NN 505 509 have have VBP 510 514 been be VBN 515 523 isolated isolate VBN 523 524 , , , 525 534 including include VBG 535 536 a a DT 537 548 heterodimer heterodimer NN 549 553 from from IN 554 564 stimulated stimulate VBN 565 571 T-cell t-cell NN 572 579 nuclear nuclear JJ 580 588 extracts extract NNS 589 599 consisting consist VBG 600 602 of of IN 603 605 Mr Mr NNP 606 607 = = JJ 608 610 90 90 CD 611 614 000 000 CD 615 616 ( ( ( 616 620 NF90 nf90 NN 620 621 ) ) ) 622 625 and and CC 626 628 Mr Mr NNP 629 630 = = JJ 631 633 45 45 CD 634 637 000 000 CD 638 639 ( ( ( 639 643 NF45 NF45 NNP 643 644 ) ) ) 645 653 subunits subunit NNS 653 654 . . . 655 658 The the DT 659 667 subunits subunit NNS 668 670 of of IN 671 675 this this DT 676 687 heterodimer heterodimer NN 688 692 have have VBP 693 697 been be VBN 698 704 cloned clone VBN 704 705 , , , 706 709 and and CC 710 714 NF90 NF90 NNP 715 718 was be VBD 719 724 found find VBN 725 727 to to TO 728 734 encode encode VB 735 736 a a DT 737 744 protein protein NN 745 755 containing contain VBG 756 759 two two CD 760 767 domains domain NNS 768 772 that that WDT 773 776 are be VBP 777 786 predicted predict VBN 787 789 to to TO 790 794 form form VB 795 801 motifs motif NNS 802 809 capable capable JJ 810 812 of of IN 813 820 binding bind VBG 821 823 to to TO 824 839 double-stranded double-stranded JJ 840 843 RNA RNA NNP 843 844 . . . 845 850 Using use VBG 851 853 in in FW 854 859 vitro vitro FW 860 870 translated translate VBN 871 883 polypeptides polypeptide NNS 883 884 , , , 885 887 we we PRP 888 892 have have VBP 893 905 demonstrated demonstrate VBN 906 910 that that IN 911 915 NF90 nf90 NN 916 928 specifically specifically RB 929 934 binds bind VBZ 935 937 to to TO 938 953 double-stranded double-stranded JJ 954 957 RNA RNA NNP 957 958 . . . 959 970 Furthermore furthermore RB 970 971 , , , 972 976 NF90 NF90 NNP 977 980 was be VBD 981 995 phosphorylated phosphorylate VBN 996 998 in in IN 999 1000 a a DT 1001 1016 double-stranded double-stranded JJ 1017 1030 RNA-dependent rna-dependent JJ 1031 1037 manner manner NN 1038 1044 likely likely RB 1045 1047 by by IN 1048 1051 the the DT 1052 1070 interferon-induced interferon-induced JJ 1070 1071 , , , 1072 1087 double-stranded double-stranded JJ 1088 1101 RNA-dependent rna-dependent JJ 1102 1109 protein protein NN 1110 1116 kinase kinase NNP 1116 1117 , , , 1118 1121 PKR PKR NNP 1121 1122 . . . 1123 1126 The the DT 1127 1131 NF90 nf90 NN 1132 1139 protein protein NN 1140 1143 was be VBD 1144 1149 found find VBN 1150 1152 to to TO 1153 1155 be be VB 1156 1165 expressed express VBN 1166 1169 not not RB 1170 1174 only only RB 1175 1177 in in IN 1178 1185 T-cells T-cell NNP 1185 1186 , , , 1187 1190 but but CC 1191 1195 also also RB 1196 1198 in in IN 1199 1208 nonimmune nonimmune JJ 1209 1213 HeLa HeLa NNP 1214 1219 cells cell NNS 1219 1220 . . . 1221 1223 In in IN 1224 1228 HeLa HeLa NNP 1229 1234 cells cell NNS 1234 1235 , , , 1236 1239 the the DT 1240 1247 protein protein NN 1248 1251 was be VBD 1252 1258 almost almost RB 1259 1270 exclusively exclusively RB 1271 1280 localized localized JJ 1281 1283 to to TO 1284 1287 the the DT 1288 1296 ribosome ribosome NN 1297 1301 salt salt NN 1302 1306 wash wash NN 1307 1315 fraction fraction NN 1316 1318 of of IN 1319 1323 cell cell NN 1324 1331 lysates lysate NNS 1331 1332 . . .