0	1	A	a	DT
2	6	role	role	NN
7	10	for	for	IN
11	17	RanBP1	ranbp1	NN
18	20	in	in	IN
21	24	the	the	DT
25	32	release	release	NN
33	35	of	of	IN
36	40	CRM1	crm1	NN
41	45	from	from	IN
46	49	the	the	DT
50	57	nuclear	nuclear	JJ
58	62	pore	pore	NN
63	70	complex	complex	NN
71	73	in	in	IN
74	75	a	a	DT
76	84	terminal	terminal	JJ
85	89	step	step	NN
90	92	of	of	IN
93	100	nuclear	nuclear	JJ
101	107	export	export	NN
107	108	.	.	.

110	112	We	we	PRP
113	121	recently	recently	RB
122	131	developed	develop	VBD
132	134	an	an	DT
135	140	assay	assay	NN
141	143	in	in	IN
144	149	which	which	WDT
150	157	nuclear	nuclear	JJ
158	164	export	export	NN
165	167	of	of	IN
168	171	the	the	DT
172	181	shuttling	shuttle	VBG
182	195	transcription	transcription	NN
196	202	factor	factor	NN
203	207	NFAT	NFAT	NNP
208	209	(	(	(
209	216	nuclear	nuclear	JJ
217	223	factor	factor	NN
224	226	of	of	IN
227	236	activated	activate	VBN
237	238	T	t	NN
239	244	cells	cell	NNS
244	245	)	)	)
246	249	can	can	MD
250	252	be	be	VB
253	266	reconstituted	reconstitute	VBN
267	269	in	in	IN
270	283	permeabilized	permeabilized	JJ
284	289	cells	cell	NNS
290	294	with	with	IN
295	298	the	the	DT
299	305	GTPase	GTPase	NNP
306	309	Ran	Ran	NNP
310	313	and	and	CC
314	317	the	the	DT
318	325	nuclear	nuclear	JJ
326	332	export	export	NN
333	341	receptor	receptor	NN
342	346	CRM1	crm1	NN
346	347	.	.	.

348	350	We	we	PRP
351	355	have	have	VBP
356	359	now	now	RB
360	364	used	use	VBN
365	369	this	this	DT
370	375	assay	assay	NN
376	378	to	to	TO
379	387	identify	identify	VB
388	395	another	another	DT
396	402	export	export	NN
403	409	factor	factor	NN
409	410	.	.	.

411	416	After	after	IN
417	430	preincubation	preincubation	NN
431	433	of	of	IN
434	447	permeabilized	permeabilized	JJ
448	453	cells	cell	NNS
454	458	with	with	IN
459	460	a	a	DT
461	464	Ran	ran	NN
465	471	mutant	mutant	NN
472	476	that	that	WDT
477	483	cannot	cannot	MD
484	493	hydrolyze	hydrolyze	VB
494	497	GTP	GTP	NNP
498	499	(	(	(
499	506	RanQ69L	RanQ69L	NNP
506	507	)	)	)
507	508	,	,	,
509	516	cytosol	cytosol	NN
517	525	supports	support	VBZ
526	530	NFAT	nfat	NN
531	537	export	export	NN
537	538	,	,	,
539	542	but	but	CC
543	547	CRM1	CRM1	NNP
548	551	and	and	CC
552	555	Ran	Ran	NNP
556	561	alone	alone	RB
562	564	do	do	VBP
565	568	not	not	RB
568	569	.	.	.

570	573	The	the	DT
574	581	RanQ69L	RanQ69L	NNP
582	595	preincubation	preincubation	NN
596	601	leads	lead	VBZ
602	604	to	to	TO
605	617	accumulation	accumulation	NN
618	620	of	of	IN
621	625	CRM1	crm1	NN
626	628	at	at	IN
629	632	the	the	DT
633	644	cytoplasmic	cytoplasmic	JJ
645	654	periphery	periphery	NN
655	657	of	of	IN
658	661	the	the	DT
662	669	nuclear	nuclear	JJ
670	674	pore	pore	NN
675	682	complex	complex	NN
683	684	(	(	(
684	687	NPC	NPC	NNP
687	688	)	)	)
689	691	in	in	IN
692	703	association	association	NN
704	708	with	with	IN
709	712	the	the	DT
713	716	p62	p62	NN
717	724	complex	complex	NN
725	728	and	and	CC
729	739	Can/Nup214	can/nup214	NN
739	740	.	.	.

741	757	RanGTP-dependent	rangtp-dependent	JJ
758	769	association	association	NN
770	772	of	of	IN
773	777	CRM1	crm1	NN
778	782	with	with	IN
783	788	these	these	DT
789	801	nucleoporins	nucleoporin	NNS
802	805	was	be	VBD
806	819	reconstituted	reconstitute	VBN
820	822	in	in	FW
823	828	vitro	vitro	FW
828	829	.	.	.

830	832	By	by	IN
833	844	biochemical	biochemical	JJ
845	858	fractionation	fractionation	NN
859	862	and	and	CC
863	877	reconstitution	reconstitution	NN
877	878	,	,	,
879	881	we	we	PRP
882	888	showed	show	VBD
889	893	that	that	IN
894	900	RanBP1	ranbp1	NN
901	909	restores	restore	VBZ
910	917	nuclear	nuclear	JJ
918	924	export	export	NN
925	930	after	after	IN
931	934	the	the	DT
935	942	RanQ69L	RanQ69L	NNP
943	956	preincubation	preincubation	NN
956	957	.	.	.

958	960	It	it	PRP
961	965	also	also	RB
966	976	stimulates	stimulate	VBZ
977	984	nuclear	nuclear	JJ
985	991	export	export	NN
992	994	in	in	IN
995	1000	cells	cell	NNS
1001	1005	that	that	WDT
1006	1010	have	have	VBP
1011	1014	not	not	RB
1015	1019	been	be	VBN
1020	1032	preincubated	preincubate	VBN
1033	1037	with	with	IN
1038	1046	RanQ69L.	RanQ69L.	NNP
1047	1053	RanBP1	RanBP1	NNP
1054	1056	as	as	RB
1057	1061	well	well	RB
1062	1064	as	as	IN
1065	1076	Ran-binding	ran-binding	JJ
1077	1084	domains	domain	NNS
1085	1087	of	of	IN
1088	1091	the	the	DT
1092	1103	cytoplasmic	cytoplasmic	JJ
1104	1115	nucleoporin	nucleoporin	NN
1116	1122	RanBP2	ranbp2	NN
1123	1130	promote	promote	VBP
1131	1134	the	the	DT
1135	1142	release	release	NN
1143	1145	of	of	IN
1146	1150	CRM1	crm1	NN
1151	1155	from	from	IN
1156	1159	the	the	DT
1160	1163	NPC	NPC	NNP
1163	1164	.	.	.

1165	1170	Taken	take	VBN
1171	1179	together	together	RB
1179	1180	,	,	,
1181	1184	our	our	PRP$
1185	1192	results	result	NNS
1193	1201	indicate	indicate	VBP
1202	1206	that	that	IN
1207	1213	RanGTP	RanGTP	NNP
1214	1216	is	be	VBZ
1217	1226	important	important	JJ
1227	1230	for	for	IN
1231	1234	the	the	DT
1235	1244	targeting	targeting	NN
1245	1247	of	of	IN
1248	1254	export	export	NN
1255	1264	complexes	complex	NNS
1265	1267	to	to	TO
1268	1271	the	the	DT
1272	1283	cytoplasmic	cytoplasmic	JJ
1284	1288	side	side	NN
1289	1291	of	of	IN
1292	1295	the	the	DT
1296	1299	NPC	NPC	NNP
1300	1303	and	and	CC
1304	1308	that	that	IN
1309	1315	RanBP1	ranbp1	NN
1316	1319	and	and	CC
1320	1328	probably	probably	RB
1329	1335	RanBP2	ranbp2	NN
1336	1339	are	be	VBP
1340	1348	involved	involve	VBN
1349	1351	in	in	IN
1352	1355	the	the	DT
1356	1368	dissociation	dissociation	NN
1369	1371	of	of	IN
1372	1379	nuclear	nuclear	JJ
1380	1386	export	export	NN
1387	1396	complexes	complex	NNS
1397	1401	from	from	IN
1402	1405	the	the	DT
1406	1409	NPC	NPC	NNP
1410	1412	in	in	IN
1413	1414	a	a	DT
1415	1423	terminal	terminal	JJ
1424	1428	step	step	NN
1429	1431	of	of	IN
1432	1441	transport	transport	NN
1441	1442	.	.	.