0	7	Effects	Effect	NNPS
8	10	of	of	IN
11	17	diesel	diesel	NN
18	25	organic	organic	JJ
26	34	extracts	extract	NNS
35	37	on	on	IN
38	47	chemokine	chemokine	NN
48	58	production	production	NN
59	61	by	by	IN
62	72	peripheral	peripheral	JJ
73	78	blood	blood	NN
79	90	mononuclear	mononuclear	JJ
91	96	cells	cell	NNS
96	97	.	.	.

99	109	BACKGROUND	background	NN
109	110	:	:	:
111	123	Polyaromatic	polyaromatic	JJ
124	136	hydrocarbons	hydrocarbon	NNS
137	138	(	(	(
138	142	PAHs	PAH	NNP
142	143	)	)	)
144	154	associated	associate	VBN
155	159	with	with	IN
160	166	diesel	diesel	NN
167	174	exhaust	exhaust	NN
175	184	particles	particle	NNS
185	186	(	(	(
186	190	DEPs	DEP	NNPS
190	191	)	)	)
192	195	are	be	VBP
196	201	found	find	VBN
202	204	in	in	IN
205	208	the	the	DT
209	220	atmospheric	atmospheric	JJ
221	226	urban	urban	JJ
227	236	pollution	pollution	NN
236	237	.	.	.

238	242	Such	such	JJ
243	252	compounds	compound	NNS
253	257	have	have	VBP
258	262	been	be	VBN
263	268	shown	show	VBN
269	271	to	to	TO
272	277	favor	favor	VB
278	281	IgE	ige	NN
282	292	production	production	NN
292	293	,	,	,
294	303	bronchial	bronchial	JJ
304	323	hyperresponsiveness	hyperresponsiveness	NN
323	324	,	,	,
325	328	and	and	CC
329	335	airway	airway	NN
336	348	inflammation	inflammation	NN
348	349	.	.	.

350	360	Chemokines	chemokine	NNS
361	364	are	be	VBP
365	366	a	a	DT
367	372	group	group	NN
373	375	of	of	IN
376	387	chemotactic	chemotactic	JJ
388	397	cytokines	cytokine	NNS
398	406	involved	involve	VBN
407	409	in	in	IN
410	413	the	the	DT
414	425	recruitment	recruitment	NN
426	428	of	of	IN
429	441	inflammatory	inflammatory	JJ
442	447	cells	cell	NNS
447	448	.	.	.

449	458	OBJECTIVE	objective	NN
458	459	:	:	:
460	462	We	we	PRP
463	475	investigated	investigate	VBD
476	479	the	the	DT
480	486	effect	effect	NN
487	489	of	of	IN
490	498	DEP-PAHs	dep-pah	NNS
499	501	on	on	IN
502	505	the	the	DT
506	513	release	release	NN
514	517	and	and	CC
518	522	mRNA	mrna	NN
523	533	expression	expression	NN
534	536	of	of	IN
537	541	IL-8	il-8	NN
541	542	,	,	,
543	548	MCP-1	mcp-1	NN
548	549	,	,	,
550	553	and	and	CC
554	560	RANTES	rantes	NN
561	563	by	by	IN
564	569	PBMCs	pbmc	NNS
570	578	obtained	obtain	VBN
579	583	from	from	IN
584	591	healthy	healthy	JJ
592	600	subjects	subject	NNS
600	601	.	.	.

602	609	METHODS	methods	NNS
609	610	:	:	:
611	618	Protein	protein	NN
619	629	production	production	NN
630	632	in	in	IN
633	645	supernatants	supernatant	NNS
646	649	was	be	VBD
650	658	assessed	assess	VBN
659	661	by	by	IN
662	667	ELISA	ELISA	NNP
667	668	,	,	,
669	672	and	and	CC
673	677	mRNA	mrna	NN
678	688	expression	expression	NN
689	692	was	be	VBD
693	702	evaluated	evaluate	VBN
703	705	by	by	IN
706	722	semiquantitative	semiquantitative	JJ
723	729	RT-PCR	rt-pcr	NN
729	730	.	.	.

731	738	RESULTS	results	NNS
738	739	:	:	:
740	749	Secretion	Secretion	NNP
750	752	of	of	IN
753	757	IL-8	IL-8	NNP
758	761	and	and	CC
762	768	RANTES	rantes	NN
769	778	increased	increase	VBD
779	781	in	in	IN
782	783	a	a	DT
784	798	dose-dependent	dose-dependent	JJ
799	805	manner	manner	NN
806	810	with	with	IN
811	821	increasing	increase	VBG
822	836	concentrations	concentration	NNS
837	839	of	of	IN
840	848	DEP-PAHs	DEP-PAH	NNP
849	850	(	(	(
850	855	range	range	NN
855	856	,	,	,
857	860	0.5	0.5	CD
861	863	ng	ng	NN
864	866	to	to	TO
867	869	50	50	CD
870	875	ng/mL	ng/ml	NN
875	876	)	)	)
876	877	.	.	.

878	880	On	on	IN
881	884	the	the	DT
885	893	contrary	contrary	NN
893	894	,	,	,
895	898	the	the	DT
899	906	release	release	NN
907	909	of	of	IN
910	915	MCP-1	mcp-1	NN
916	919	was	be	VBD
920	933	significantly	significantly	RB
934	943	inhibited	inhibit	VBN
943	944	,	,	,
945	949	also	also	RB
950	952	in	in	IN
953	954	a	a	DT
955	969	dose-dependent	dose-dependent	JJ
970	976	manner	manner	NN
976	977	.	.	.

978	987	Messenger	messenger	NN
988	991	RNA	RNA	NNP
992	1002	production	production	NN
1003	1009	coding	cod	VBG
1010	1013	for	for	IN
1014	1018	IL-8	il-8	NN
1018	1019	,	,	,
1020	1026	RANTES	RANTES	NNP
1026	1027	,	,	,
1028	1031	and	and	CC
1032	1037	MCP-1	mcp-1	NN
1038	1044	showed	show	VBD
1045	1053	parallel	parallel	JJ
1054	1064	variations	variation	NNS
1065	1067	to	to	TO
1068	1071	the	the	DT
1072	1082	production	production	NN
1083	1085	of	of	IN
1086	1089	the	the	DT
1090	1103	correspondent	correspondent	JJ
1104	1112	proteins	protein	NNS
1112	1113	.	.	.

1114	1121	Effects	Effect	NNPS
1122	1124	of	of	IN
1125	1133	DEP-PAHs	DEP-PAH	NNP
1134	1140	became	become	VBD
1141	1152	significant	significant	JJ
1153	1155	at	at	IN
1156	1157	7	7	CD
1158	1163	hours	hour	NNS
1164	1167	and	and	CC
1168	1170	up	up	RB
1171	1173	to	to	TO
1174	1176	48	48	CD
1177	1182	hours	hour	NNS
1183	1187	time	time	NN
1188	1195	culture	culture	NN
1196	1199	for	for	IN
1200	1205	MCP-1	mcp-1	NN
1205	1206	,	,	,
1207	1210	and	and	CC
1211	1213	up	up	RB
1214	1216	to	to	TO
1217	1219	24	24	CD
1220	1225	hours	hour	NNS
1226	1230	time	time	NN
1231	1238	culture	culture	NN
1239	1242	for	for	IN
1243	1247	IL-8	il-8	NN
1248	1251	and	and	CC
1252	1258	RANTES	RANTES	NNP
1258	1259	.	.	.

1260	1268	Moreover	moreover	RB
1268	1269	,	,	,
1270	1282	supernatants	supernatant	NNS
1283	1287	from	from	IN
1288	1305	DEP-PAH-activated	dep-pah-activated	JJ
1306	1311	cells	cell	NNS
1311	1312	,	,	,
1313	1321	compared	compare	VBN
1322	1326	with	with	IN
1327	1332	those	those	DT
1333	1335	of	of	IN
1336	1344	controls	control	NNS
1344	1345	,	,	,
1346	1355	exhibited	exhibit	VBD
1356	1357	a	a	DT
1358	1371	significantly	significantly	RB
1372	1380	enhanced	enhance	VBN
1381	1392	chemotactic	chemotactic	JJ
1393	1401	activity	activity	NN
1402	1405	for	for	IN
1406	1417	neutrophils	neutrophil	NNS
1418	1421	and	and	CC
1422	1433	eosinophils	eosinophil	NNS
1433	1434	,	,	,
1435	1440	which	which	WDT
1441	1444	was	be	VBD
1445	1458	significantly	significantly	RB
1459	1468	inhibited	inhibit	VBN
1469	1471	by	by	IN
1472	1484	pretreatment	pretreatment	NN
1485	1489	with	with	IN
1490	1499	anti-IL-8	anti-il-8	JJ
1500	1503	and	and	CC
1504	1515	anti-RANTES	anti-rantes	JJ
1516	1528	neutralizing	neutralize	VBG
1529	1539	antibodies	antibody	NNS
1539	1540	,	,	,
1541	1553	respectively	respectively	RB
1553	1554	.	.	.

1555	1565	CONCLUSION	conclusion	NN
1565	1566	:	:	:
1567	1572	These	these	DT
1573	1581	findings	finding	NNS
1582	1589	suggest	suggest	VBP
1590	1594	that	that	IN
1595	1598	the	the	DT
1599	1608	chemokine	chemokine	NN
1609	1617	pathways	pathway	NNS
1618	1621	are	be	VBP
1622	1631	modulated	modulate	VBN
1632	1634	by	by	IN
1635	1643	DEP-PAHs	dep-pah	NNS
1644	1646	at	at	IN
1647	1650	the	the	DT
1651	1666	transcriptional	transcriptional	JJ
1667	1672	level	level	NN
1672	1673	,	,	,
1674	1685	reinforcing	reinforce	VBG
1686	1689	the	the	DT
1690	1694	idea	idea	NN
1695	1699	that	that	IN
1700	1703	the	the	DT
1704	1715	development	development	NN
1716	1718	of	of	IN
1719	1731	inflammatory	inflammatory	JJ
1732	1741	reactions	reaction	NNS
1742	1747	might	might	MD
1748	1750	be	be	VB
1751	1759	affected	affect	VBN
1760	1762	by	by	IN
1763	1769	diesel	diesel	NN
1770	1777	exhaust	exhaust	NN
1778	1786	emission	emission	NN
1786	1787	.	.	.