0 9 Oxidative oxidative JJ 10 16 stress stress NN 17 25 triggers trigger VBZ 26 31 STAT3 stat3 NN 32 40 tyrosine tyrosine NN 41 56 phosphorylation phosphorylation NN 57 60 and and CC 61 68 nuclear nuclear JJ 69 82 translocation translocation NN 83 85 in in IN 86 91 human human JJ 92 103 lymphocytes lymphocyte NNS 103 104 . . . 106 115 Oxidizing oxidize VBG 116 122 agents agent NNS 123 126 are be VBP 127 135 powerful powerful JJ 136 146 activators activator NNS 147 149 of of IN 150 157 factors factor NNS 158 169 responsible responsible JJ 170 173 for for IN 174 177 the the DT 178 193 transcriptional transcriptional JJ 194 204 activation activation NN 205 207 of of IN 208 225 cytokine-encoding cytokine-encoding JJ 226 231 genes gene NNS 232 240 involved involve VBN 241 243 in in IN 244 250 tissue tissue NN 251 257 injury injury NN 257 258 . . . 259 261 In in IN 262 266 this this DT 267 272 study study NN 273 275 we we PRP 276 280 show show VBP 281 289 evidence evidence NN 290 294 that that IN 295 300 STAT3 STAT3 NNP 301 303 is be VBZ 304 305 a a DT 306 319 transcription transcription NN 320 326 factor factor NN 327 332 whose whose WP$ 333 341 activity activity NN 342 344 is be VBZ 345 354 modulated modulate VBN 355 357 by by IN 358 362 H2O2 h2o2 NN 363 365 in in IN 366 371 human human JJ 372 383 lymphocytes lymphocyte NNS 383 384 , , , 385 387 in in IN 388 393 which which WDT 394 404 endogenous endogenous JJ 405 413 catalase catalase NN 414 417 had have VBD 418 428 previously previously RB 429 433 been be VBN 434 443 inhibited inhibit VBN 443 444 . . . 445 457 H2O2-induced h2o2-induced JJ 458 465 nuclear nuclear JJ 466 479 translocation translocation NN 480 482 of of IN 483 488 STAT3 STAT3 NNP 489 491 to to TO 492 496 form form VB 497 514 sequence-specific sequence-specific JJ 515 524 DNA-bound dna-bound JJ 525 534 complexes complex NNS 535 538 was be VBD 539 548 evidenced evidence VBN 549 551 by by IN 552 566 immunoblotting immunoblotting NN 567 569 of of IN 570 577 nuclear nuclear JJ 578 587 fractions fraction NNS 588 591 and and CC 592 607 electrophoretic electrophoretic JJ 608 616 mobility mobility NN 617 622 shift shift NN 623 629 assays assay NNS 629 630 , , , 631 634 and and CC 635 643 vanadate vanadate NN 644 647 was be VBD 648 653 found find VBN 654 656 to to TO 657 665 strongly strongly RB 666 675 synergize synergize VB 676 680 with with IN 681 685 H2O2 h2o2 NN 685 686 . . . 687 695 Moreover moreover RB 695 696 , , , 697 707 anti-STAT3 anti-stat3 JJ 708 718 antibodies antibody NNS 719 731 specifically specifically RB 732 744 precipitated precipitate VBD 745 746 a a DT 747 754 protein protein NN 755 757 of of IN 758 760 92 92 CD 761 764 kDa kda NN 765 769 that that WDT 770 777 becomes become VBZ 778 792 phosphorylated phosphorylate VBN 793 795 on on IN 796 804 tyrosine tyrosine NN 805 809 upon upon IN 810 820 lymphocyte lymphocyte NN 821 830 treatment treatment NN 831 835 with with IN 836 840 H2O2 h2o2 NN 840 841 . . . 842 854 Phenylarsine Phenylarsine NNP 855 860 oxide oxide NN 860 861 , , , 862 863 a a DT 864 872 tyrosine tyrosine NN 873 884 phosphatase phosphatase NN 885 894 inhibitor inhibitor NN 894 895 , , , 896 899 and and CC 900 909 genistein genistein NN 909 910 , , , 911 912 a a DT 913 921 tyrosine tyrosine NN 922 928 kinase kinase NNP 929 938 inhibitor inhibitor NN 938 939 , , , 940 950 cooperated cooperate VBD 951 954 and and CC 955 964 cancelled cancel VBD 964 965 , , , 966 978 respectively respectively RB 978 979 , , , 980 983 the the DT 984 997 H2O2-promoted h2o2-promoted JJ 998 1003 STAT3 stat3 NN 1004 1011 nuclear nuclear JJ 1012 1025 translocation translocation NN 1025 1026 . . . 1027 1035 Evidence evidence NN 1036 1038 is be VBZ 1039 1043 also also RB 1044 1053 presented present VBN 1053 1054 , , , 1055 1060 using use VBG 1061 1070 Fe2+/Cu2+ fe2+/cu2+ JJ 1071 1075 ions ion NNS 1075 1076 , , , 1077 1084 that.OH that.oh NN 1085 1094 generated generate VBN 1095 1099 from from IN 1100 1104 H2O2 h2o2 NN 1105 1112 through through IN 1113 1119 Fenton Fenton NNP 1120 1129 reactions reaction NNS 1130 1135 could could MD 1136 1138 be be VB 1139 1140 a a DT 1141 1150 candidate candidate NN 1151 1157 oxygen oxygen NN 1158 1166 reactive reactive JJ 1167 1174 species specie NNS 1175 1177 to to TO 1178 1186 directly directly RB 1187 1195 activate activate VB 1196 1201 STAT3 stat3 NN 1201 1202 . . . 1203 1210 Present present JJ 1211 1215 data datum NNS 1216 1223 suggest suggest VBP 1224 1228 that that IN 1229 1233 H2O2 h2o2 NN 1234 1237 and and CC 1238 1246 vanadate vanadate NN 1247 1250 are be VBP 1251 1257 likely likely JJ 1258 1260 to to TO 1261 1268 inhibit inhibit VB 1269 1272 the the DT 1273 1281 activity activity NN 1282 1284 of of IN 1285 1298 intracellular intracellular JJ 1299 1307 tyrosine tyrosine NN 1308 1322 phosphatase(s) phosphatase(s) NN 1322 1323 , , , 1324 1331 leading lead VBG 1332 1334 to to TO 1335 1343 enhanced enhance VBN 1344 1349 STAT3 stat3 NN 1350 1358 tyrosine tyrosine NN 1359 1374 phosphorylation phosphorylation NN 1375 1378 and and CC 1379 1384 hence hence RB 1385 1388 its its PRP$ 1389 1402 translocation translocation NN 1403 1405 to to TO 1406 1409 the the DT 1410 1417 nucleus nucleus NN 1417 1418 . . . 1419 1424 These these DT 1425 1432 results result NNS 1433 1444 demonstrate demonstrate VBP 1445 1449 that that IN 1450 1453 the the DT 1454 1457 DNA dna NN 1458 1465 binding binding NN 1466 1474 activity activity NN 1475 1477 of of IN 1478 1483 STAT3 STAT3 NNP 1484 1487 can can MD 1488 1490 be be VB 1491 1500 modulated modulate VBN 1501 1503 by by IN 1504 1513 oxidizing oxidize VBG 1514 1520 agents agent NNS 1521 1524 and and CC 1525 1532 provide provide VBP 1533 1534 a a DT 1535 1544 framework framework NN 1545 1547 to to TO 1548 1558 understand understand VB 1559 1562 the the DT 1563 1570 effects effect NNS 1571 1573 of of IN 1574 1583 oxidative oxidative JJ 1584 1590 stress stress NN 1591 1593 on on IN 1594 1597 the the DT 1598 1606 JAK-STAT jak-stat NN 1607 1616 signaling signaling NN 1617 1624 pathway pathway NN 1624 1625 . . .