0 6 Aiolos Aiolos NNP 7 20 transcription transcription NN 21 27 factor factor NN 28 36 controls control VBZ 37 41 cell cell NN 42 47 death death NN 48 50 in in IN 51 52 T t NN 53 58 cells cell NNS 59 61 by by IN 62 72 regulating regulate VBG 73 78 Bcl-2 bcl-2 NN 79 89 expression expression NN 90 93 and and CC 94 97 its its PRP$ 98 106 cellular cellular JJ 107 119 localization localization NN 119 120 . . . 122 124 We we PRP 125 133 searched search VBD 134 137 for for IN 138 146 proteins protein NNS 147 151 that that WDT 152 160 interact interact VBP 161 165 with with IN 166 169 Ras ras NN 170 172 in in IN 173 184 interleukin interleukin NN 185 186 ( ( ( 186 202 IL)-2-stimulated il)-2-stimulated JJ 203 205 or or CC 206 219 IL-2-deprived il-2-deprived JJ 220 225 cells cell NNS 225 226 , , , 227 230 and and CC 231 236 found find VBD 237 241 that that IN 242 245 the the DT 246 259 transcription transcription NN 260 266 factor factor NN 267 273 Aiolos Aiolos NNP 274 283 interacts interact VBZ 284 288 with with IN 289 292 Ras Ras NNP 292 293 . . . 294 297 The the DT 298 308 Ras-Aiolos Ras-Aiolos NNP 309 320 interaction interaction NN 321 324 was be VBD 325 334 confirmed confirm VBN 335 337 in in FW 338 343 vitro vitro FW 344 347 and and CC 348 350 in in FW 351 355 vivo vivo FW 356 358 by by IN 359 381 co-immunoprecipitation co-immunoprecipitation NN 381 382 . . . 383 391 Indirect indirect JJ 392 410 immunofluorescence immunofluorescence NN 411 416 shows show VBZ 417 421 that that IN 422 426 IL-2 il-2 NN 427 435 controls control VBZ 436 439 the the DT 440 448 cellular cellular JJ 449 461 distribution distribution NN 462 464 of of IN 465 471 Aiolos Aiolos NNP 472 475 and and CC 476 483 induces induce VBZ 484 487 its its PRP$ 488 496 tyrosine tyrosine NN 497 512 phosphorylation phosphorylation NN 512 513 , , , 514 522 required require VBN 523 526 for for IN 527 539 dissociation dissociation NN 540 544 from from IN 545 548 Ras Ras NNP 548 549 . . . 550 552 We we PRP 553 557 also also RB 558 568 identified identify VBD 569 579 functional functional JJ 580 594 Aiolos-binding aiolos-binding JJ 595 600 sites site NNS 601 603 in in IN 604 607 the the DT 608 613 Bcl-2 bcl-2 NN 614 622 promoter promoter NN 622 623 , , , 624 629 which which WDT 630 633 are be VBP 634 638 able able JJ 639 641 to to TO 642 650 activate activate VB 651 654 the the DT 655 665 luciferase luciferase NN 666 674 reporter reporter NN 675 679 gene gene NN 679 680 . . . 681 689 Mutation mutation NN 690 692 of of IN 693 707 Aiolos-binding aiolos-binding JJ 708 713 sites site NNS 714 720 within within IN 721 724 the the DT 725 730 Bcl-2 bcl-2 NN 731 739 promoter promoter NN 740 748 inhibits inhibit VBZ 749 764 transactivation transactivation NN 765 767 of of IN 768 771 the the DT 772 780 reporter reporter NN 781 785 gene gene NN 786 796 luciferase luciferase NN 796 797 , , , 798 808 suggesting suggest VBG 809 815 direct direct JJ 816 823 control control NN 824 826 of of IN 827 832 Bcl-2 bcl-2 NN 833 843 expression expression NN 844 846 by by IN 847 853 Aiolos Aiolos NNP 853 854 . . . 855 870 Co-transfection co-transfection NN 871 882 experiments experiment NNS 883 890 confirm confirm VBP 891 895 that that IN 896 902 Aiolos Aiolos NNP 903 910 induces induce VBZ 911 916 Bcl-2 bcl-2 NN 917 927 expression expression NN 928 931 and and CC 932 940 prevents prevent VBZ 941 950 apoptosis apoptosis NN 951 953 in in IN 954 967 IL-2-deprived il-2-deprived JJ 968 973 cells cell NNS 973 974 . . . 975 977 We we PRP 978 985 propose propose VBP 986 987 a a DT 988 993 model model NN 994 997 for for IN 998 1001 the the DT 1002 1012 regulation regulation NN 1013 1015 of of IN 1016 1021 Bcl-2 bcl-2 NN 1022 1032 expression expression NN 1033 1036 via via IN 1037 1043 Aiolos Aiolos NNP 1043 1044 . . .