0 3 The the DT 4 8 role role NN 9 11 of of IN 12 23 gamma/delta gamma/delta NN 24 25 T t NN 26 30 cell cell NN 31 39 receptor receptor NN 40 48 positive positive JJ 49 54 cells cell NNS 55 57 in in IN 58 67 pregnancy pregnancy NN 67 68 . . . 70 77 PROBLEM problem NN 77 78 : : : 79 82 Due due JJ 83 85 to to TO 86 89 the the DT 90 94 lack lack NN 95 97 of of IN 98 107 classical classical JJ 108 111 HLA HLA NNP 112 120 antigens antigen NNS 121 123 on on IN 124 127 the the DT 128 139 trophoblast trophoblast NN 139 140 , , , 141 146 fetal fetal JJ 147 155 antigens antigen NNS 156 159 are be VBP 160 168 possibly possibly RB 169 178 presented present VBN 179 181 in in IN 182 183 a a DT 184 187 non non JJ 188 193 major major JJ 194 212 histocompatibility histocompatibility NN 213 220 complex complex NN 221 222 ( ( ( 222 225 MHC MHC NNP 225 226 ) ) ) 227 237 restricted restricted JJ 238 241 way way NN 241 242 . . . 243 251 Decidual decidual JJ 252 262 gammadelta gammadelta NN 263 264 T t NN 265 270 cells cell NNS 270 271 , , , 272 277 which which WDT 278 291 significantly significantly RB 292 300 increase increase VBP 301 303 in in IN 304 310 number number NN 311 317 during during IN 318 327 pregnancy pregnancy NN 327 328 , , , 329 334 might might MD 335 339 play play VB 340 341 a a DT 342 346 role role NN 347 349 in in IN 350 361 recognition recognition NN 362 364 of of IN 365 370 fetal fetal JJ 371 379 antigens antigen NNS 380 383 and and CC 384 388 also also RB 389 391 in in IN 392 403 determining determine VBG 404 407 the the DT 408 415 quality quality NN 416 418 of of IN 419 422 the the DT 423 431 response response NN 432 434 to to TO 435 440 these these DT 441 449 antigens antigen NNS 449 450 . . . 451 454 Our our PRP$ 455 460 study study NN 461 464 was be VBD 465 470 aimed aim VBN 471 473 at at IN 474 487 investigating investigate VBG 488 491 the the DT 492 496 role role NN 497 499 of of IN 500 504 this this DT 505 509 cell cell NN 510 520 population population NN 521 523 in in IN 524 546 progesterone-dependent progesterone-dependent JJ 547 563 immunomodulation immunomodulation NN 563 564 . . . 565 571 METHOD method NN 572 574 OF of IN 575 580 STUDY STUDY NNP 580 581 : : : 582 592 Peripheral peripheral JJ 593 604 lymphocytes lymphocyte NNS 605 609 from from IN 610 617 healthy healthy JJ 618 626 pregnant pregnant JJ 627 632 women woman NNS 633 636 and and CC 637 641 from from IN 642 650 habitual habitual JJ 651 659 aborters aborter NNS 660 664 were be VBD 665 671 tested test VBN 672 674 by by IN 675 694 immunocytochemistry immunocytochemistry NN 695 698 for for IN 699 702 the the DT 703 711 presence presence NN 712 714 of of IN 715 726 gamma/delta gamma/delta NN 727 728 T t NN 729 733 cell cell NN 734 742 receptor receptor NN 743 744 ( ( ( 744 747 TCR TCR NNP 747 748 ) ) ) 749 752 and and CC 753 765 progesterone progesterone NN 766 774 receptor receptor NN 774 775 . . . 776 778 To to TO 779 790 investigate investigate VB 791 794 the the DT 795 801 effect effect NN 802 804 of of IN 805 814 treatment treatment NN 815 819 with with IN 820 821 a a DT 822 825 pan pan NN 826 830 anti anti JJ 831 842 gamma/delta gamma/delta NN 843 851 antibody antibody NN 851 852 , , , 853 864 lymphocytes lymphocyte NNS 865 869 were be VBD 870 879 incubated incubate VBN 880 883 for for IN 884 885 3 3 CD 886 888 hr hr NN 889 893 with with IN 894 897 the the DT 898 906 antibody antibody NN 906 907 , , , 908 911 and and CC 912 916 then then RB 917 928 interleukin interleukin NN 929 930 ( ( ( 930 936 IL)-10 IL)-10 NNP 936 937 , , , 938 943 IL-12 il-12 NN 944 947 and and CC 948 968 progesterone-induced progesterone-induced JJ 969 977 blocking block VBG 978 984 factor factor NN 985 986 ( ( ( 986 990 PIBF PIBF NNP 990 991 ) ) ) 992 1002 expression expression NN 1003 1004 ( ( ( 1004 1006 by by IN 1007 1027 immuno-cytochemistry immuno-cytochemistry NN 1027 1028 ) ) ) 1029 1031 as as RB 1032 1036 well well RB 1037 1039 as as IN 1040 1047 natural natural JJ 1048 1054 killer killer NN 1055 1056 ( ( ( 1056 1058 NK NK NNP 1058 1059 ) ) ) 1060 1064 cell cell NN 1065 1073 activity activity NN 1074 1078 were be VBD 1079 1089 determined determine VBN 1089 1090 . . . 1091 1098 RESULTS results NNS 1098 1099 : : : 1100 1102 In in IN 1103 1113 peripheral peripheral JJ 1114 1119 blood blood NN 1120 1122 of of IN 1123 1130 healthy healthy JJ 1131 1139 pregnant pregnant JJ 1140 1145 women woman NNS 1146 1149 the the DT 1150 1160 percentage percentage NN 1161 1163 of of IN 1164 1175 gamma/delta gamma/delta NN 1176 1180 TCR+ tcr+ JJ 1181 1186 cells cell NNS 1187 1190 was be VBD 1191 1204 significantly significantly RB 1205 1211 higher high JJR 1212 1213 ( ( ( 1213 1214 P p NN 1215 1216 < < SYM 1217 1222 0.001 0.001 CD 1222 1223 ) ) ) 1224 1228 than than IN 1229 1231 in in IN 1232 1236 that that DT 1237 1239 of of IN 1240 1249 recurrent recurrent JJ 1250 1258 aborters aborter NNS 1259 1261 or or CC 1262 1264 of of IN 1265 1277 non-pregnant non-pregnant JJ 1278 1289 individuals individual NNS 1289 1290 . . . 1291 1303 Ninety-seven ninety-seven CD 1304 1311 percent percent NN 1312 1314 of of IN 1315 1326 gamma/delta gamma/delta NN 1327 1331 TCR+ tcr+ JJ 1332 1341 pregnancy pregnancy NN 1342 1353 lymphocytes lymphocyte NNS 1354 1363 expressed express VBD 1364 1376 progesterone progesterone NN 1377 1385 receptor receptor NN 1385 1386 . . . 1387 1394 Binding binding NN 1395 1397 of of IN 1398 1399 a a DT 1400 1408 specific specific JJ 1409 1417 antibody antibody NN 1418 1420 to to TO 1421 1424 the the DT 1425 1436 gamma/delta gamma/delta NN 1437 1440 TCR TCR NNP 1441 1450 inhibited inhibit VBD 1451 1456 PIBF- pibf- NN 1457 1459 as as RB 1460 1464 well well RB 1465 1467 as as IN 1468 1473 IL-10 il-10 NN 1474 1484 production production NN 1484 1485 , , , 1486 1493 whereas whereas IN 1494 1496 it it PRP 1497 1506 increased increase VBD 1507 1509 NK NK NNP 1510 1518 activity activity NN 1519 1522 and and CC 1523 1528 IL-12 il-12 NN 1529 1539 expression expression NN 1539 1540 . . . 1541 1552 CONCLUSIONS conclusions NNS 1552 1553 : : : 1554 1559 These these DT 1560 1564 data datum NNS 1565 1572 suggest suggest VBP 1573 1576 the the DT 1577 1581 role role NN 1582 1584 of of IN 1585 1596 gamma/delta gamma/delta NN 1597 1608 TCR-bearing tcr-bearing JJ 1609 1620 lymphocytes lymphocyte NNS 1621 1623 in in IN 1624 1646 progesterone-dependent progesterone-dependent JJ 1647 1663 immunomodulation immunomodulation NN 1663 1664 . . .