0 10 Diminished diminish VBN 11 20 responses response NNS 21 23 to to TO 24 29 IL-13 il-13 NN 30 32 by by IN 33 38 human human JJ 39 48 monocytes monocyte NNS 49 63 differentiated differentiate VBN 64 66 in in FW 67 72 vitro vitro FW 72 73 : : : 74 78 role role NN 79 81 of of IN 82 85 the the DT 86 98 IL-13Ralpha1 il-13ralpha1 NN 99 104 chain chain NN 105 108 and and CC 109 114 STAT6 stat6 NN 114 115 . . . 117 120 The the DT 121 128 primary primary JJ 129 134 IL-13 il-13 NN 135 143 receptor receptor NN 144 151 complex complex NN 152 154 on on IN 155 160 human human JJ 161 170 monocytes monocyte NNS 171 173 is be VBZ 174 182 believed believe VBN 183 185 to to TO 186 188 be be VB 189 190 a a DT 191 202 heterodimer heterodimer NN 203 212 comprised comprise VBN 213 215 of of IN 216 219 the the DT 220 225 IL-4R IL-4R NNP 226 231 alpha alpha NN 232 237 chain chain NN 238 241 and and CC 242 245 the the DT 246 251 IL-2R IL-2R NNP 252 257 gamma gamma NN 258 263 chain chain NN 264 265 ( ( ( 265 279 gamma(c))-like gamma(c))-like JJ 280 288 molecule molecule NN 288 289 , , , 290 296 IL-13R IL-13R NNP 297 304 alpha1. alpha1. NN 305 309 mRNA mrna NN 310 316 levels level NNS 317 320 for for IN 321 327 IL-13R IL-13R NNP 328 334 alpha1 alpha1 NN 334 335 , , , 336 339 but but CC 340 343 not not RB 344 349 IL-4R il-4r NN 350 355 alpha alpha NN 355 356 , , , 357 361 were be VBD 362 370 markedly markedly RB 371 380 decreased decrease VBN 381 383 in in IN 384 386 in in FW 387 392 vitro vitro FW 393 409 monocyte-derived monocyte-derived JJ 410 421 macrophages macrophage NNS 422 423 ( ( ( 423 428 MDMac MDMac NNP 428 429 ) ) ) 429 430 , , , 431 434 and and CC 435 439 with with IN 440 450 increasing increase VBG 451 455 time time NN 456 458 of of IN 459 468 monocytes monocyte NNS 469 471 in in IN 472 479 culture culture NN 480 490 correlated correlate VBD 491 495 with with IN 496 499 the the DT 500 504 loss loss NN 505 507 of of IN 508 513 IL-13 il-13 NN 514 524 regulation regulation NN 525 527 of of IN 528 554 lipopolysaccharide-induced lipopolysaccharide-induced JJ 555 564 TNF-alpha TNF-alpha NNP 565 575 production production NN 575 576 . . . 577 585 Analysis Analysis NNP 586 588 of of IN 589 593 cell cell NN 594 599 lines line NNS 600 605 Daudi Daudi NNP 606 609 and and CC 610 615 THP-1 thp-1 NN 616 620 that that WDT 621 635 differentially differentially RB 636 643 express express VBP 644 652 gamma(c) gamma(c) NNP 653 656 and and CC 657 663 IL-13R IL-13R NNP 664 670 alpha1 alpha1 NN 671 677 showed show VBD 678 682 that that IN 683 688 IL-13 il-13 NN 689 692 can can MD 693 701 activate activate VB 702 707 STAT6 stat6 NN 708 710 in in IN 711 717 IL-13R IL-13R NNP 718 733 alpha1-positive alpha1-positive JJ 734 739 THP-1 thp-1 NN 740 745 cells cell NNS 746 749 but but CC 750 753 not not RB 754 756 in in IN 757 774 gamma(c)-positive gamma(c)-positive JJ 774 775 , , , 776 782 IL-13R IL-13R NNP 783 798 alpha1-negative alpha1-negative JJ 799 804 Daudi Daudi NNP 805 810 cells cell NNS 810 811 . . . 812 817 IL-13 il-13 NN 818 828 activation activation NN 829 831 of of IN 832 837 STAT6 STAT6 NNP 838 841 was be VBD 842 849 reduced reduce VBN 850 852 in in IN 853 858 MDMac MDMac NNP 859 864 which which WDT 865 868 was be VBD 869 879 associated associate VBN 880 884 with with IN 885 895 diminished diminish VBN 896 909 IL-13-induced il-13-induced JJ 910 920 expression expression NN 921 923 of of IN 924 928 CD23 CD23 NNP 929 932 and and CC 933 936 MHC MHC NNP 937 942 class class NN 943 945 II ii CD 945 946 . . . 947 954 However however RB 954 955 , , , 956 960 with with IN 961 968 reduced reduce VBN 969 975 IL-13R IL-13R NNP 976 982 alpha1 alpha1 NN 983 993 expression expression NN 994 997 and and CC 998 1001 low low JJ 1002 1009 nuclear nuclear JJ 1010 1015 STAT6 stat6 NN 1016 1024 activity activity NN 1024 1025 , , , 1026 1030 some some DT 1031 1044 IL-13-induced il-13-induced JJ 1045 1054 responses response NNS 1055 1059 were be VBD 1060 1069 unaltered unaltered JJ 1070 1072 in in IN 1073 1082 magnitude magnitude NN 1083 1085 in in IN 1086 1091 MDMac MDMac NNP 1091 1092 . . . 1093 1095 In in IN 1096 1099 the the DT 1100 1107 absence absence NN 1108 1110 of of IN 1111 1121 functional functional JJ 1122 1128 IL-13R IL-13R NNP 1129 1135 alpha1 alpha1 NN 1136 1139 and and CC 1140 1148 gamma(c) gamma(c) NNP 1148 1149 , , , 1150 1155 IL-13 il-13 NN 1156 1160 must must MD 1161 1167 signal signal VB 1168 1175 through through IN 1176 1178 an an DT 1179 1190 alternative alternative JJ 1191 1199 receptor receptor NN 1200 1207 complex complex NN 1208 1210 on on IN 1211 1216 MDMac MDMac NNP 1216 1217 . . . 1218 1229 Experiments experiment NNS 1230 1234 with with IN 1235 1236 a a DT 1237 1245 blocking block VBG 1246 1254 antibody antibody NN 1255 1257 to to TO 1258 1263 IL-4R IL-4R NNP 1264 1269 alpha alpha NN 1270 1276 showed show VBD 1277 1281 that that IN 1282 1286 this this DT 1287 1292 chain chain NN 1293 1300 remains remain VBZ 1301 1303 an an DT 1304 1313 essential essential JJ 1314 1323 component component NN 1324 1326 of of IN 1327 1330 the the DT 1331 1336 IL-13 il-13 NN 1337 1345 receptor receptor NN 1346 1353 complex complex NN 1354 1356 on on IN 1357 1362 MDMac MDMac NNP 1362 1363 . . .