0	23	Charybdotoxin-sensitive	charybdotoxin-sensitive	JJ
23	24	,	,	,
25	41	Ca(2+)-dependent	ca(2+)-dependent	JJ
42	50	membrane	membrane	NN
51	60	potential	potential	NN
61	68	changes	change	NNS
69	72	are	be	VBP
73	76	not	not	RB
77	85	involved	involve	VBN
86	88	in	in	IN
89	94	human	human	JJ
95	96	T	t	NN
97	99	or	or	CC
100	101	B	B	NNP
102	106	cell	cell	NN
107	117	activation	activation	NN
118	121	and	and	CC
122	135	proliferation	proliferation	NN
135	136	.	.	.

138	141	The	the	DT
142	153	involvement	involvement	NN
154	156	of	of	IN
157	160	ion	ion	NN
161	169	channels	channel	NNS
170	172	in	in	IN
173	174	B	B	NNP
175	178	and	and	CC
179	180	T	t	NN
181	191	lymphocyte	lymphocyte	NN
192	202	activation	activation	NN
203	205	is	be	VBZ
206	215	supported	support	VBN
216	218	by	by	IN
219	223	many	many	JJ
224	231	reports	report	NNS
232	234	of	of	IN
235	242	changes	change	NNS
243	245	in	in	IN
246	249	ion	ion	NN
250	256	fluxes	flux	NNS
257	260	and	and	CC
261	269	membrane	membrane	NN
270	279	potential	potential	NN
280	285	after	after	IN
286	293	mitogen	mitogen	NN
294	301	binding	binding	NN
301	302	.	.	.

303	308	Human	human	JJ
309	310	T	t	NN
311	314	and	and	CC
315	316	B	b	NN
317	328	lymphocytes	lymphocyte	NNS
329	340	demonstrate	demonstrate	VBP
341	343	an	an	DT
344	349	early	early	JJ
350	353	and	and	CC
354	363	transient	transient	JJ
364	381	hyperpolarization	hyperpolarization	NN
382	387	after	after	IN
388	394	ligand	ligand	NN
395	402	binding	binding	NN
402	403	.	.	.

404	412	Inasmuch	inasmuch	RB
413	415	as	as	IN
416	419	the	the	DT
420	426	change	change	NN
427	429	in	in	IN
430	438	membrane	membrane	NN
439	448	potential	potential	NN
449	451	is	be	VBZ
452	461	dependent	dependent	JJ
462	464	on	on	IN
465	474	elevation	elevation	NN
475	477	of	of	IN
478	482	free	free	JJ
483	492	cytosolic	cytosolic	JJ
493	500	calcium	calcium	NN
500	501	,	,	,
502	505	the	the	DT
506	523	hyperpolarization	hyperpolarization	NN
524	526	is	be	VBZ
527	537	presumably	presumably	RB
538	545	through	through	IN
546	553	opening	opening	NN
554	556	of	of	IN
557	574	Ca(2+)-stimulated	ca(2+)-stimulated	JJ
575	577	K+	k+	NN
578	586	channels	channel	NNS
586	587	.	.	.

588	590	We	we	PRP
591	595	have	have	VBP
596	600	used	use	VBN
601	614	charybdotoxin	charybdotoxin	NN
614	615	,	,	,
616	617	a	a	DT
618	623	known	known	JJ
624	633	inhibitor	inhibitor	NN
634	636	of	of	IN
637	653	Ca(2+)-dependent	ca(2+)-dependent	JJ
654	656	K+	K+	NNP
657	665	channels	channel	NNS
665	666	,	,	,
667	669	to	to	TO
670	675	study	study	VB
676	679	the	the	DT
680	684	role	role	NN
685	687	of	of	IN
688	693	these	these	DT
694	702	channels	channel	NNS
703	705	in	in	IN
706	716	lymphocyte	lymphocyte	NN
717	727	activation	activation	NN
728	731	and	and	CC
732	743	mitogenesis	mitogenesis	NN
743	744	.	.	.

745	747	We	we	PRP
748	759	demonstrate	demonstrate	VBP
760	764	that	that	IN
765	778	charybdotoxin	charybdotoxin	NN
779	787	inhibits	inhibit	VBZ
788	791	the	the	DT
792	806	ligand-induced	ligand-induced	JJ
807	816	transient	transient	JJ
817	825	membrane	membrane	NN
826	843	hyperpolarization	hyperpolarization	NN
844	846	in	in	IN
847	848	B	B	NNP
849	852	and	and	CC
853	854	T	t	NN
855	860	cells	cell	NNS
861	863	in	in	IN
864	865	a	a	DT
866	880	dose-dependent	dose-dependent	JJ
881	888	fashion	fashion	NN
888	889	,	,	,
890	897	without	without	IN
898	907	affecting	affect	VBG
908	915	changes	change	NNS
916	918	in	in	IN
919	928	cytosolic	cytosolic	JJ
929	933	Ca2+	ca2+	NN
933	934	.	.	.

935	942	However	however	RB
942	943	,	,	,
944	952	blockade	blockade	NN
953	955	of	of	IN
956	959	the	the	DT
960	976	Ca(2+)-activated	ca(2+)-activated	JJ
977	979	K+	k+	NN
980	987	channel	channel	NN
988	990	is	be	VBZ
991	994	not	not	RB
995	1005	associated	associate	VBN
1006	1010	with	with	IN
1011	1018	changes	change	NNS
1019	1021	in	in	IN
1022	1032	cell-cycle	cell-cycle	NN
1033	1037	gene	gene	NN
1038	1048	activation	activation	NN
1048	1049	,	,	,
1050	1054	IL-2	il-2	NN
1055	1065	production	production	NN
1065	1066	,	,	,
1067	1072	IL-2R	IL-2R	NNP
1073	1083	expression	expression	NN
1084	1086	or	or	CC
1087	1088	B	B	NNP
1089	1092	and	and	CC
1093	1094	T	t	NN
1095	1099	cell	cell	NN
1100	1111	mitogenesis	mitogenesis	NN
1111	1112	.	.	.

1113	1118	These	these	DT
1119	1126	results	result	NNS
1127	1132	imply	imply	VBP
1133	1137	that	that	IN
1138	1146	membrane	membrane	NN
1147	1156	potential	potential	NN
1157	1164	changes	change	NNS
1165	1174	secondary	secondary	JJ
1175	1177	to	to	TO
1178	1181	the	the	DT
1182	1198	ligand-dependent	ligand-dependent	JJ
1199	1206	opening	opening	NN
1207	1209	of	of	IN
1210	1226	Ca(2+)-activated	ca(2+)-activated	JJ
1227	1229	K+	k+	NN
1230	1238	channels	channel	NNS
1239	1242	are	be	VBP
1243	1246	not	not	RB
1247	1255	involved	involve	VBN
1256	1258	in	in	IN
1259	1260	B	B	NNP
1261	1264	and	and	CC
1265	1266	T	t	NN
1267	1277	lymphocyte	lymphocyte	NN
1278	1288	activation	activation	NN
1289	1292	and	and	CC
1293	1304	mitogenesis	mitogenesis	NN
1304	1305	.	.	.