0 7 Cloning cloning NN 8 10 of of IN 11 17 murine murine JJ 18 23 TCF-1 tcf-1 NN 23 24 , , , 25 26 a a DT 27 28 T t NN 29 42 cell-specific cell-specific JJ 43 56 transcription transcription NN 57 63 factor factor NN 64 75 interacting interact VBG 76 80 with with IN 81 91 functional functional JJ 92 98 motifs motif NNS 99 101 in in IN 102 105 the the DT 106 117 CD3-epsilon CD3-epsilon NNP 118 121 and and CC 122 123 T t NN 124 128 cell cell NN 129 137 receptor receptor NN 138 143 alpha alpha NN 144 153 enhancers enhancer NNS 153 154 . . . 156 167 CD3-epsilon CD3-epsilon NNP 168 172 gene gene NN 173 183 expression expression NN 184 186 is be VBZ 187 195 confined confine VBN 196 198 to to TO 199 202 the the DT 203 204 T t NN 205 209 cell cell NN 210 217 lineage lineage NN 217 218 . . . 219 221 We we PRP 222 226 have have VBP 227 235 recently recently RB 236 246 identified identify VBN 247 250 and and CC 251 257 cloned clone VBN 258 259 a a DT 260 265 human human JJ 266 279 transcription transcription NN 280 286 factor factor NN 286 287 , , , 288 293 TCF-1 TCF-1 NNP 293 294 , , , 295 299 that that WDT 300 305 binds bind VBZ 306 308 to to TO 309 310 a a DT 311 321 functional functional JJ 322 329 element element NN 330 332 in in IN 333 336 the the DT 337 338 T t NN 339 358 lymphocyte-specific lymphocyte-specific JJ 359 367 enhancer enhancer NN 368 370 of of IN 371 382 CD3-epsilon CD3-epsilon NNP 382 383 . . . 384 386 In in IN 387 388 a a DT 389 394 panel panel NN 395 397 of of IN 398 403 human human JJ 404 408 cell cell NN 409 414 lines line NNS 414 415 , , , 416 421 TCF-1 tcf-1 NN 422 432 expression expression NN 433 436 was be VBD 437 447 restricted restricted JJ 448 450 to to TO 451 452 T t NN 453 460 lineage lineage NN 461 466 cells cell NNS 466 467 . . . 468 473 TCF-1 TCF-1 NNP 474 482 belonged belong VBD 483 485 to to TO 486 487 a a DT 488 493 novel novel JJ 494 500 family family NN 501 503 of of IN 504 509 genes gene NNS 510 514 that that WDT 515 522 contain contain VBP 523 526 the the DT 527 536 so-called so-called JJ 537 541 high high JJ 542 550 mobility mobility NN 551 556 group group NN 557 558 1 1 CD 559 560 ( ( ( 560 563 HMG HMG NNP 563 564 ) ) ) 565 568 box box NN 568 569 . . . 570 574 Here here RB 575 577 we we PRP 578 584 report report VBP 585 588 the the DT 589 596 cloning cloning NN 597 599 of of IN 600 606 murine murine JJ 607 612 TCF-1 tcf-1 NN 612 613 . . . 614 617 Two two CD 618 624 splice splice NN 625 637 alternatives alternative NNS 638 642 were be VBD 643 653 identified identify VBN 654 658 that that WDT 659 663 were be VBD 664 667 not not RB 668 678 previously previously RB 679 687 observed observe VBN 688 690 in in IN 691 696 human human JJ 697 702 TCF-1 tcf-1 NN 702 703 . . . 704 710 Murine murine JJ 711 714 and and CC 715 720 human human JJ 721 726 TCF-1 tcf-1 NN 727 736 displayed display VBD 737 738 a a DT 739 743 95.5 95.5 CD 743 744 % % NN 745 752 overall overall JJ 753 758 amino amino JJ 759 763 acid acid NN 764 772 homology homology NN 772 773 . . . 774 785 Recombinant recombinant JJ 786 792 murine murine JJ 793 796 and and CC 797 802 human human JJ 803 808 TCF-1 tcf-1 NN 809 819 recognized recognize VBD 820 823 the the DT 824 828 same same JJ 829 837 sequence sequence NN 838 843 motif motif NN 844 846 in in IN 847 850 the the DT 851 862 CD3-epsilon CD3-epsilon NNP 863 871 enhancer enhancer NN 872 874 as as IN 875 881 judged judge VBN 882 884 by by IN 885 888 gel gel NN 889 900 retardation retardation NN 901 904 and and CC 905 916 methylation methylation NN 917 929 interference interference NN 930 936 assays assay NNS 936 937 . . . 938 942 With with IN 943 946 the the DT 947 953 murine murine JJ 954 958 cDNA cdna NN 959 965 clones clone NNS 966 973 several several JJ 974 981 aspects aspect NNS 982 984 of of IN 985 990 TCF-1 TCF-1 NNP 991 995 were be VBD 996 1004 analyzed analyze VBN 1004 1005 . . . 1006 1011 First first RB 1011 1012 , , , 1013 1021 deletion deletion NN 1022 1030 analysis analysis NN 1031 1039 revealed reveal VBD 1040 1044 that that IN 1045 1046 a a DT 1047 1053 region region NN 1054 1056 of of IN 1057 1062 TCF-1 tcf-1 NN 1063 1073 containing contain VBG 1074 1077 the the DT 1078 1081 HMG HMG NNP 1082 1085 box box NN 1086 1089 was be VBD 1090 1100 sufficient sufficient JJ 1101 1104 for for IN 1105 1122 sequence-specific sequence-specific JJ 1123 1130 binding binding NN 1130 1131 . . . 1132 1138 Second second RB 1138 1139 , , , 1140 1142 by by IN 1143 1147 high high JJ 1148 1158 stringency stringency NN 1159 1167 Northern Northern NNP 1168 1176 blotting blotting NN 1177 1180 and and CC 1181 1183 in in FW 1184 1188 situ situ FW 1189 1202 hybridization hybridization NN 1202 1203 , , , 1204 1209 TCF-1 tcf-1 NN 1210 1220 expression expression NN 1221 1224 was be VBD 1225 1230 shown show VBN 1231 1233 to to TO 1234 1236 be be VB 1237 1245 confined confine VBN 1246 1248 to to TO 1249 1252 the the DT 1253 1259 thymus thymus NN 1260 1263 and and CC 1264 1266 to to TO 1267 1270 the the DT 1271 1272 T t NN 1273 1277 cell cell NN 1278 1283 areas area NNS 1284 1286 of of IN 1287 1290 the the DT 1291 1297 spleen spleen NN 1297 1298 . . . 1299 1304 Third third RB 1304 1305 , , , 1306 1311 TCF-1 tcf-1 NN 1312 1317 bound bind VBD 1318 1330 specifically specifically RB 1331 1333 to to TO 1334 1335 a a DT 1336 1346 functional functional JJ 1347 1348 T t NN 1349 1362 cell-specific cell-specific JJ 1363 1370 element element NN 1371 1373 in in IN 1374 1377 the the DT 1378 1379 T t NN 1380 1384 cell cell NN 1385 1393 receptor receptor NN 1394 1399 alpha alpha NN 1400 1401 ( ( ( 1401 1410 TCR-alpha TCR-alpha NNP 1410 1411 ) ) ) 1412 1420 enhancer enhancer NN 1420 1421 . . . 1422 1425 The the DT 1426 1427 T t NN 1428 1444 lineage-specific lineage-specific JJ 1445 1455 expression expression NN 1456 1459 and and CC 1460 1463 the the DT 1464 1472 affinity affinity NN 1473 1476 for for IN 1477 1487 functional functional JJ 1488 1494 motifs motif NNS 1495 1497 in in IN 1498 1501 the the DT 1502 1511 TCR-alpha TCR-alpha NNP 1512 1515 and and CC 1516 1527 CD3-epsilon CD3-epsilon NNP 1528 1537 enhancers enhancer NNS 1538 1543 imply imply VBP 1544 1546 an an DT 1547 1556 important important JJ 1557 1561 role role NN 1562 1565 for for IN 1566 1571 TCF-1 tcf-1 NN 1572 1574 in in IN 1575 1578 the the DT 1579 1592 establishment establishment NN 1593 1595 of of IN 1596 1599 the the DT 1600 1606 mature mature JJ 1607 1608 T t NN 1609 1613 cell cell NN 1614 1623 phenotype phenotype NN 1623 1624 . . .