0 12 Constitutive constitutive JJ 13 23 expression expression NN 24 26 of of IN 27 32 HIV-1 HIV-1 NNP 33 36 tat tat NN 37 44 protein protein NN 45 47 in in IN 48 53 human human JJ 54 60 Jurkat Jurkat NNP 61 62 T t NN 63 68 cells cell NNS 69 74 using use VBG 75 76 a a DT 77 79 BK BK NNP 80 85 virus virus NN 86 92 vector vector NN 92 93 . . . 95 98 The the DT 99 109 production production NN 110 113 and and CC 114 130 characterization characterization NN 131 133 of of IN 134 140 Jurkat Jurkat NNP 141 145 cell cell NN 146 151 lines line NNS 152 156 that that WDT 157 171 constitutively constitutively RB 172 179 express express VBP 180 190 functional functional JJ 191 196 human human JJ 197 203 immune immune JJ 204 214 deficiency deficiency NN 215 220 virus virus NN 221 225 type type NN 226 227 1 1 CD 228 229 ( ( ( 229 234 HIV-1 HIV-1 NNP 234 235 ) ) ) 236 239 tat tat NN 240 247 protein protein NN 247 248 , , , 249 254 using use VBG 255 256 a a DT 257 259 BK BK NNP 260 265 virus virus NN 266 273 plasmid plasmid NN 274 284 expression expression NN 285 291 vector vector NN 292 295 and and CC 296 301 HIV-1 HIV-1 NNP 302 305 tat tat NN 306 310 cDNA cDNA NNP 310 311 , , , 312 314 is be VBZ 315 324 described describe VBN 324 325 . . . 326 328 An an DT 329 338 increased increase VBN 339 345 growth growth NN 346 350 rate rate NN 351 353 of of IN 354 359 these these DT 360 370 Jurkat-tat Jurkat-tat NNP 371 375 cell cell NN 376 381 lines line NNS 382 384 as as IN 385 393 compared compare VBN 394 398 with with IN 399 406 control control NN 407 411 cell cell NN 412 417 lines line NNS 418 421 was be VBD 422 430 observed observe VBN 430 431 . . .