0 14 Megakaryocytic megakaryocytic JJ 15 18 and and CC 19 31 erythrocytic erythrocytic JJ 32 40 lineages lineage NNS 41 46 share share VBP 47 55 specific specific JJ 56 69 transcription transcription NN 70 77 factors factor NNS 77 78 . . . 80 98 Erythroid-specific erythroid-specific JJ 99 104 genes gene NNS 105 112 contain contain VBP 113 120 binding bind VBG 121 126 sites site NNS 127 130 for for IN 131 136 NF-E1 nf-e1 NN 137 138 ( ( ( 138 142 also also RB 143 149 called call VBN 150 154 GF-1 gf-1 NN 155 158 and and CC 159 165 Eryf-1 eryf-1 NN 165 166 ; ; : 167 171 refs ref NNS 172 175 1-3 1-3 CD 176 188 respectively respectively RB 188 189 ) ) ) 189 190 , , , 191 194 the the DT 195 204 principal principal JJ 205 216 DNA-binding dna-binding JJ 217 224 protein protein NN 225 227 of of IN 228 231 the the DT 232 244 erythrocytic erythrocytic JJ 245 252 lineage lineage NN 252 253 . . . 254 259 NF-E1 nf-e1 NN 260 270 expression expression NN 271 276 seems seem VBZ 277 279 to to TO 280 282 be be VB 283 293 restricted restricted JJ 294 296 to to TO 297 300 the the DT 301 313 erythrocytic erythrocytic JJ 314 321 lineage lineage NN 321 322 . . . 323 324 A a DT 325 332 closely closely RB 333 340 related related JJ 341 342 ( ( ( 342 344 if if IN 345 348 not not RB 349 358 identical identical JJ 358 359 ) ) ) 360 367 protein protein NN 368 370 is be VBZ 371 376 found find VBN 377 379 in in IN 380 384 both both CC 385 386 a a DT 387 392 human human JJ 393 407 megakaryocytic megakaryocytic JJ 408 412 cell cell NN 413 417 line line NN 418 421 and and CC 422 430 purified purify VBN 431 436 human human JJ 437 451 megakaryocytes megakaryocyte NNS 451 452 ; ; : 453 455 it it PRP 456 461 binds bind VBZ 462 464 to to TO 465 473 promoter promoter NN 474 481 regions region NNS 482 484 of of IN 485 488 two two CD 489 512 megakaryocytic-specific megakaryocytic-specific JJ 513 518 genes gene NNS 518 519 . . . 520 523 The the DT 524 531 binding bind VBG 532 537 sites site NNS 538 541 and and CC 542 549 partial partial JJ 550 561 proteolysis proteolysis NN 562 569 profile profile NN 570 572 of of IN 573 577 this this DT 578 585 protein protein NN 586 589 are be VBP 590 607 indistinguishable indistinguishable JJ 608 612 from from IN 613 618 those those DT 619 621 of of IN 622 625 the the DT 626 635 erythroid erythroid JJ 636 643 protein protein NN 643 644 ; ; : 645 649 also also RB 649 650 , , , 651 656 NF-E1 nf-e1 NN 657 666 messenger messenger NN 667 670 RNA RNA NNP 671 673 is be VBZ 674 677 the the DT 678 682 same same JJ 683 687 size size NN 688 690 in in IN 691 695 both both CC 696 699 the the DT 700 714 megakaryocytic megakaryocytic JJ 715 718 and and CC 719 728 erythroid erythroid JJ 729 733 cell cell NN 734 739 lines line NNS 739 740 . . . 741 752 Furthermore furthermore RB 752 753 , , , 754 759 point point NN 760 769 mutations mutation NNS 770 774 that that WDT 775 782 abolish abolish VBP 783 790 binding binding NN 791 793 of of IN 794 799 NF-E1 nf-e1 NN 800 806 result result VBP 807 809 in in IN 810 811 a a DT 812 814 70 70 CD 814 815 % % NN 816 824 decrease decrease NN 825 827 in in IN 828 831 the the DT 832 847 transcriptional transcriptional JJ 848 856 activity activity NN 857 859 of of IN 860 861 a a DT 862 885 megakaryocytic-specific megakaryocytic-specific JJ 886 894 promoter promoter NN 894 895 . . . 896 898 We we PRP 899 903 also also RB 904 908 find find VBP 909 913 that that IN 914 919 NF-E2 nf-e2 NN 919 920 , , , 921 928 another another DT 929 941 trans-acting trans-acting JJ 942 948 factor factor NN 949 951 of of IN 952 955 the the DT 956 968 erythrocytic erythrocytic JJ 969 976 lineage lineage NN 976 977 , , , 978 980 is be VBZ 981 988 present present JJ 989 991 in in IN 992 1006 megakaryocytes megakaryocyte NNS 1006 1007 . . . 1008 1023 Transcriptional transcriptional JJ 1024 1031 effects effect NNS 1032 1034 in in IN 1035 1039 both both DT 1040 1048 lineages lineage NNS 1049 1054 might might MD 1055 1059 then then RB 1060 1062 be be VB 1063 1071 mediated mediate VBN 1072 1074 in in IN 1075 1079 part part NN 1080 1082 by by IN 1083 1086 the the DT 1087 1091 same same JJ 1092 1100 specific specific JJ 1101 1113 trans-acting trans-acting JJ 1114 1121 factors factor NNS 1121 1122 . . . 1123 1126 Our our PRP$ 1127 1131 data datum NNS 1132 1142 strengthen strengthen VBP 1143 1146 the the DT 1147 1151 idea idea NN 1152 1154 of of IN 1155 1156 a a DT 1157 1162 close close JJ 1163 1174 association association NN 1175 1182 between between IN 1183 1186 the the DT 1187 1199 erythrocytic erythrocytic JJ 1200 1203 and and CC 1204 1207 the the DT 1208 1222 megakaryocytic megakaryocytic JJ 1223 1231 lineages lineage NNS 1232 1235 and and CC 1236 1241 could could MD 1242 1246 also also RB 1247 1254 explain explain VB 1255 1258 the the DT 1259 1269 expression expression NN 1270 1272 of of IN 1273 1280 markers marker NNS 1281 1289 specific specific JJ 1290 1292 to to TO 1293 1296 the the DT 1297 1309 erythrocytic erythrocytic JJ 1310 1313 and and CC 1314 1328 megakaryocytic megakaryocytic JJ 1329 1337 lineages lineage NNS 1338 1340 in in IN 1341 1345 most most JJS 1346 1360 erythroblastic erythroblastic JJ 1361 1364 and and CC 1365 1381 megakaryoblastic megakaryoblastic JJ 1382 1391 permanent permanent JJ 1392 1396 cell cell NN 1397 1402 lines line NNS 1402 1403 . . .