0 3 The the DT 4 10 murine murine JJ 11 15 BCL6 bcl6 NN 16 20 gene gene NN 21 23 is be VBZ 24 31 induced induce VBN 32 34 in in IN 35 44 activated activate VBN 45 56 lymphocytes lymphocyte NNS 57 59 as as IN 60 62 an an DT 63 72 immediate immediate JJ 73 78 early early JJ 79 83 gene gene NN 83 84 . . . 86 89 The the DT 90 101 chromosomal chromosomal JJ 102 115 translocation translocation NN 116 125 involving involve VBG 126 130 3q27 3q27 NN 131 133 is be VBZ 134 139 often often RB 140 148 detected detect VBN 149 151 in in IN 152 157 human human JJ 158 164 B-cell b-cell NN 165 174 lymphomas lymphoma NNS 174 175 , , , 176 186 especially especially RB 187 194 diffuse diffuse JJ 195 204 lymphomas lymphoma NNS 205 209 with with IN 210 211 a a DT 212 222 large-cell large-cell JJ 223 232 component component NN 232 233 . . . 234 237 The the DT 238 242 BCL6 BCL6 NNP 243 247 gene gene NN 248 251 has have VBZ 252 256 been be VBN 257 265 isolated isolate VBN 266 270 from from IN 271 274 the the DT 275 286 chromosomal chromosomal JJ 287 297 breakpoint breakpoint NN 298 300 in in IN 301 306 these these DT 307 316 lymphomas lymphoma NNS 316 317 . . . 318 322 Here here RB 323 325 we we PRP 326 332 cloned clone VBD 333 336 the the DT 337 343 murine murine JJ 344 348 BCL6 bcl6 NN 349 350 ( ( ( 350 355 mBCL6 mbcl6 NN 355 356 ) ) ) 357 361 cDNA cdna NN 362 366 from from IN 367 370 the the DT 371 377 muscle muscle NN 378 382 cDNA cdna NN 383 390 library library NN 391 396 using use VBG 397 400 the the DT 401 406 human human JJ 407 411 BCL6 bcl6 NN 412 413 ( ( ( 413 418 hBCL6 hbcl6 NN 418 419 ) ) ) 420 424 cDNA cdna NN 425 427 as as IN 428 429 a a DT 430 435 probe probe NN 435 436 . . . 437 440 The the DT 441 450 predicted predict VBN 451 456 amino amino NN 457 461 acid acid NN 462 470 sequence sequence NN 471 474 was be VBD 475 477 95 95 CD 477 478 % % NN 479 488 identical identical JJ 489 491 to to TO 492 496 that that DT 497 499 of of IN 500 505 hBCL6 hbcl6 NN 505 506 . . . 507 509 It it PRP 510 518 contains contain VBZ 519 522 six six CD 523 530 repeats repeat NNS 531 533 of of IN 534 537 the the DT 538 550 Kruppel-like kruppel-like JJ 551 562 zinc-finger zinc-finger NN 563 568 motif motif NN 569 573 that that WDT 574 577 are be VBP 578 588 completely completely RB 589 598 identical identical JJ 599 601 to to TO 602 607 those those DT 608 610 of of IN 611 616 hBCL6 hbcl6 NN 616 617 , , , 618 628 indicating indicate VBG 629 633 that that IN 634 637 the the DT 638 642 BCL6 BCL6 NNP 643 647 gene gene NN 648 650 is be VBZ 651 655 well well RB 656 665 conserved conserve VBN 666 673 between between IN 674 680 humans human NNS 681 684 and and CC 685 689 mice mouse NNS 689 690 . . . 691 701 Expression expression NN 702 704 of of IN 705 708 the the DT 709 714 mBCL6 mbcl6 NN 715 719 gene gene NN 720 723 was be VBD 724 736 ubiquitously ubiquitously RB 737 745 detected detect VBN 746 748 in in IN 749 754 adult adult JJ 755 760 mouse mouse NN 761 768 tissues tissue NNS 769 778 including include VBG 779 788 lymphatic lymphatic JJ 789 795 organs organ NNS 795 796 . . . 797 808 Furthermore furthermore RB 808 809 , , , 810 812 it it PRP 813 816 was be VBD 817 824 induced induce VBN 825 827 in in IN 828 839 lymphocytes lymphocyte NNS 840 849 activated activate VBN 850 854 with with IN 855 862 phorbol phorbol NN 863 868 ester ester NN 869 872 and and CC 873 877 Ca2+ ca2+ NN 878 887 ionophore ionophore NN 888 894 within within IN 895 897 30 30 CD 898 901 min min NN 902 907 after after IN 908 919 stimulation stimulation NN 919 920 . . . 921 925 This this DT 926 935 induction induction NN 936 939 was be VBD 940 943 not not RB 944 953 inhibited inhibit VBN 954 956 by by IN 957 966 treatment treatment NN 967 969 of of IN 970 973 the the DT 974 979 cells cell NNS 980 984 with with IN 985 986 a a DT 987 994 protein protein NN 995 1004 synthesis synthesis NN 1005 1014 inhibitor inhibitor NN 1014 1015 , , , 1016 1029 cycloheximide cycloheximide NN 1029 1030 . . . 1031 1036 These these DT 1037 1044 results result NNS 1045 1052 suggest suggest VBP 1053 1057 that that IN 1058 1062 BCL6 BCL6 NNP 1063 1068 plays play VBZ 1069 1070 a a DT 1071 1075 role role NN 1076 1078 in in IN 1079 1088 activated activate VBN 1089 1100 lymphocytes lymphocyte NNS 1101 1103 as as IN 1104 1106 an an DT 1107 1116 immediate immediate JJ 1117 1122 early early JJ 1123 1127 gene gene NN 1127 1128 . . .