0 10 Inhibition Inhibition NNP 11 13 of of IN 14 27 dexamethasone dexamethasone NN 28 35 binding binding NN 36 38 to to TO 39 44 human human JJ 45 59 glucocorticoid glucocorticoid NN 60 68 receptor receptor NN 69 71 by by IN 72 75 New New NNP 76 81 World World NNP 82 89 primate primate JJ 90 94 cell cell NN 95 103 extracts extract NNS 103 104 . . . 106 108 To to TO 109 118 determine determine VB 119 121 if if IN 122 125 New new JJ 126 131 World World NNP 132 140 primates primate NNS 141 148 express express VBP 149 151 an an DT 152 161 inhibitor inhibitor NN 162 166 that that WDT 167 177 influences influence VBZ 178 192 glucocorticoid glucocorticoid NN 193 201 receptor receptor NN 202 203 ( ( ( 203 205 GR GR NNP 205 206 ) ) ) 207 214 binding binding NN 215 230 characteristics characteristic NNS 230 231 , , , 232 234 we we PRP 235 243 examined examine VBD 244 245 [ [ ( 245 261 3H]dexamethasone 3H]dexamethasone NNP 262 269 binding binding NN 270 272 in in IN 273 280 cytosol cytosol NN 281 289 prepared prepare VBN 290 294 from from IN 295 300 B95-8 b95-8 NN 301 309 lymphoid lymphoid JJ 310 315 cells cell NNS 315 316 , , , 317 324 derived derive VBN 325 329 from from IN 330 333 the the DT 334 340 cotton cotton NN 341 344 top top NN 345 352 tamarin tamarin NN 353 354 ( ( ( 354 362 Saguinus saguinus NN 363 370 oedipus oedipus NN 370 371 ) ) ) 371 372 , , , 373 375 in in IN 376 387 combination combination NN 388 392 with with IN 393 400 cytosol cytosol NN 401 409 prepared prepare VBN 410 414 from from IN 415 420 human human JJ 421 423 or or CC 424 427 rat rat NN 428 435 tissues tissue NNS 435 436 . . . 437 442 B95-8 b95-8 NN 443 450 cytosol cytosol NN 451 460 inhibited inhibit VBD 461 469 specific specific JJ 470 477 binding binding NN 478 480 of of IN 481 482 [ [ ( 482 498 3H]dexamethasone 3H]dexamethasone NNP 499 500 ( ( ( 500 501 P p NN 502 503 < < SYM 504 508 0.01 0.01 CD 508 509 ) ) ) 510 514 when when WRB 515 520 mixed mix VBN 521 525 with with IN 526 533 cytosol cytosol NN 534 542 prepared prepare VBN 543 547 from from IN 548 554 either either CC 555 556 a a DT 557 562 human human JJ 563 571 lymphoid lymphoid JJ 572 576 cell cell NN 577 581 line line NN 582 583 ( ( ( 583 585 HL HL NNP 585 586 ) ) ) 587 589 or or CC 590 593 rat rat NN 594 600 thymus thymus NN 600 601 . . . 602 605 The the DT 606 616 inhibitory inhibitory JJ 617 625 activity activity NN 626 629 was be VBD 630 634 heat heat NN 635 641 labile labile JJ 642 645 and and CC 646 653 trypsin trypsin NN 654 663 sensitive sensitive JJ 663 664 . . . 665 669 Peak peak JJ 670 680 inhibitory inhibitory JJ 681 689 activity activity NN 690 693 was be VBD 694 699 found find VBN 700 702 in in IN 703 706 the the DT 707 714 150-200 150-200 CD 715 717 kd kd NN 718 727 fractions fraction NNS 728 733 after after IN 734 743 Sephacryl Sephacryl NNP 744 749 G-200 G-200 NNP 750 765 ultrafiltration ultrafiltration NN 765 766 . . . 767 776 Scatchard Scatchard NNP 777 785 analysis analysis NN 786 788 of of IN 789 790 [ [ ( 790 806 3H]dexamethasone 3H]dexamethasone NNP 807 814 binding binding NN 815 820 using use VBG 821 826 mixed mixed JJ 827 834 cytosol cytosol NN 835 841 showed show VBD 842 843 a a DT 844 854 diminished diminish VBN 855 857 GR gr NN 858 866 apparent apparent JJ 867 874 binding binding NN 875 883 affinity affinity NN 884 888 when when WRB 889 897 compared compare VBN 898 900 to to TO 901 903 HL hl NN 904 911 cytosol cytosol NN 911 912 . . . 913 920 Kinetic kinetic JJ 921 928 studies study NNS 929 934 using use VBG 935 940 mixed mixed JJ 941 948 cytosol cytosol NN 949 958 indicated indicate VBD 959 963 that that IN 964 969 B95-8 b95-8 NN 970 977 cytosol cytosol NN 978 981 did do VBD 982 985 not not RB 986 992 affect affect VB 993 996 the the DT 997 1005 apparent apparent JJ 1006 1018 dissociation dissociation NN 1019 1023 rate rate NN 1024 1026 of of IN 1027 1028 [ [ ( 1028 1044 3H]dexamethasone 3H]dexamethasone NNP 1044 1045 . . . 1046 1051 These these DT 1052 1056 data datum NNS 1057 1068 demonstrate demonstrate VBP 1069 1073 that that IN 1074 1079 B95-8 b95-8 NN 1080 1085 cells cell NNS 1086 1093 contain contain VBP 1094 1095 a a DT 1096 1107 competitive competitive JJ 1108 1117 inhibitor inhibitor NN 1118 1122 that that WDT 1123 1131 prevents prevent VBZ 1132 1139 binding binding NN 1140 1142 of of IN 1143 1156 dexamethasone dexamethasone NN 1157 1159 to to TO 1160 1163 its its PRP$ 1164 1171 cognate cognate JJ 1172 1180 receptor receptor NN 1180 1181 . . .