0 13 Interleukin-2 interleukin-2 NN 14 21 induces induce VBZ 22 30 tyrosine tyrosine NN 31 46 phosphorylation phosphorylation NN 47 50 and and CC 51 58 nuclear nuclear JJ 59 72 translocation translocation NN 73 75 of of IN 76 81 stat3 stat3 NN 82 84 in in IN 85 90 human human JJ 91 92 T t NN 93 104 lymphocytes lymphocyte NNS 104 105 . . . 107 109 An an DT 110 115 early early JJ 116 127 biochemical biochemical JJ 128 133 event event NN 134 144 associated associate VBN 145 149 with with IN 150 151 T t NN 152 156 cell cell NN 157 167 activation activation NN 168 175 through through IN 176 179 the the DT 180 193 interleukin-2 interleukin-2 NN 194 202 receptor receptor NN 203 204 ( ( ( 204 209 IL-2R IL-2R NNP 209 210 ) ) ) 211 213 is be VBZ 214 222 tyrosine tyrosine NN 223 238 phosphorylation phosphorylation NN 239 241 of of IN 242 249 several several JJ 250 263 intracellular intracellular JJ 264 274 substrates substrate NNS 274 275 . . . 276 279 The the DT 280 285 exact exact JJ 286 295 mechanism mechanism NN 296 298 by by IN 299 304 which which WDT 305 309 IL-2 il-2 NN 310 319 regulates regulate VBZ 320 333 transcription transcription NN 334 336 of of IN 337 346 different different JJ 347 352 genes gene NNS 353 355 is be VBZ 356 365 presently presently RB 366 373 unknown unknown JJ 373 374 . . . 375 379 Here here RB 379 380 , , , 381 383 we we PRP 384 390 report report VBP 391 395 that that IN 396 407 stimulation stimulation NN 408 415 through through IN 416 419 the the DT 420 425 IL-2R il-2r NN 426 433 induced induced JJ 434 442 tyrosine tyrosine NN 443 458 phosphorylation phosphorylation NN 459 462 and and CC 463 473 subsequent subsequent JJ 474 481 nuclear nuclear JJ 482 495 translocation translocation NN 496 498 of of IN 499 504 stat3 stat3 NN 504 505 , , , 506 507 a a DT 508 513 newly newly RB 514 524 identified identify VBN 525 531 member member NN 532 534 of of IN 535 538 the the DT 539 545 signal signal NN 546 557 transducers transducer NNS 558 561 and and CC 562 572 activators activator NNS 573 575 of of IN 576 589 transcription transcription NN 590 591 ( ( ( 591 595 STAT STAT NNP 595 596 ) ) ) 597 603 family family NN 604 606 of of IN 607 615 proteins protein NNS 615 616 . . . 617 619 In in IN 620 628 contrast contrast NN 628 629 , , , 630 635 stat1 stat1 NN 636 644 proteins protein NNS 645 649 were be VBD 650 653 not not RB 654 662 tyrosine tyrosine NN 663 677 phosphorylated phosphorylated JJ 678 683 after after IN 684 688 IL-2 il-2 NN 689 697 ligation ligation NN 697 698 , , , 699 706 whereas whereas IN 707 730 tyrosine-phosphorylated tyrosine-phosphorylated JJ 731 736 stat1 stat1 NN 737 745 proteins protein NNS 746 747 ( ( ( 747 749 91 91 CD 750 753 and and CC 754 756 84 84 CD 757 760 kDa kda NN 761 769 proteins protein NNS 769 770 ) ) ) 771 775 were be VBD 776 788 translocated translocate VBN 789 791 to to TO 792 795 the the DT 796 803 nucleus nucleus NN 804 813 following follow VBG 814 830 interferon-gamma interferon-gamma NN 831 840 treatment treatment NN 841 843 of of IN 844 848 HeLa HeLa NNP 849 854 cells cell NNS 854 855 . . . 856 861 Apart apart RB 862 866 from from IN 867 872 stat3 stat3 NN 872 873 , , , 874 881 another another DT 882 893 cytoplasmic cytoplasmic JJ 894 901 protein protein NN 902 905 was be VBD 906 914 tyrosine tyrosine NN 915 929 phosphorylated phosphorylated JJ 930 933 and and CC 934 946 subsequently subsequently RB 947 959 translocated translocate VBN 960 962 to to TO 963 966 the the DT 967 974 nucleus nucleus NN 975 977 in in IN 978 986 response response NN 987 989 to to TO 990 994 IL-2 il-2 NN 994 995 . . . 996 1000 This this DT 1001 1008 protein protein NN 1009 1012 had have VBD 1013 1015 an an DT 1016 1024 apparent apparent JJ 1025 1034 molecular molecular JJ 1035 1039 mass mass NN 1040 1042 of of IN 1043 1045 84 84 CD 1046 1049 kDa kda NN 1050 1053 and and CC 1054 1057 was be VBD 1058 1061 not not RB 1062 1072 recognized recognize VBN 1073 1075 by by IN 1076 1081 stat3 stat3 NN 1082 1084 or or CC 1085 1090 stat1 stat1 NN 1091 1094 mAb mab NN 1095 1097 or or CC 1098 1106 antisera antiserum NNS 1106 1107 . . . 1108 1113 Since since IN 1114 1118 IL-2 il-2 NN 1119 1126 induced induce VBD 1127 1134 nuclear nuclear JJ 1135 1148 translocation translocation NN 1149 1151 of of IN 1152 1155 the the DT 1156 1158 84 84 CD 1159 1162 kDa kda NN 1163 1170 protein protein NN 1171 1174 and and CC 1175 1180 stat3 stat3 NN 1181 1189 followed follow VBD 1190 1199 identical identical JJ 1200 1208 kinetics kinetic NNS 1208 1209 , , , 1210 1213 p84 p84 NN 1214 1216 is be VBZ 1217 1218 a a DT 1219 1228 candidate candidate NN 1229 1232 for for IN 1233 1234 a a DT 1235 1238 new new JJ 1238 1239 , , , 1240 1243 yet yet RB 1244 1253 undefined undefined JJ 1253 1254 , , , 1255 1261 member member NN 1262 1264 of of IN 1265 1268 the the DT 1269 1273 STAT STAT NNP 1274 1280 family family NN 1280 1281 . . . 1282 1287 Taken take VBN 1288 1296 together together RB 1296 1297 , , , 1298 1300 we we PRP 1301 1307 report report VBP 1308 1312 that that IN 1313 1317 IL-2 il-2 NN 1318 1325 induces induce VBZ 1326 1334 tyrosine tyrosine NN 1335 1350 phosphorylation phosphorylation NN 1351 1354 and and CC 1355 1365 subsequent subsequent JJ 1366 1373 nuclear nuclear JJ 1374 1387 translocation translocation NN 1388 1390 of of IN 1391 1396 stat3 stat3 NN 1397 1400 and and CC 1401 1403 an an DT 1404 1406 as as RB 1407 1410 yet yet RB 1411 1420 undefined undefined JJ 1421 1427 84-kDa 84-kda JJ 1428 1435 protein protein NN 1436 1438 in in IN 1439 1455 antigen-specific antigen-specific JJ 1456 1461 human human JJ 1462 1463 T t NN 1464 1468 cell cell NN 1469 1474 lines line NNS 1474 1475 . . .