0 9 Induction Induction NNP 10 12 of of IN 13 19 ICAM-1 ICAM-1 NNP 20 23 and and CC 24 29 LFA-3 LFA-3 NNP 30 32 by by IN 33 37 Tax1 tax1 NN 38 40 of of IN 41 46 human human JJ 47 53 T-cell t-cell NN 54 62 leukemia leukemia NN 63 68 virus virus NN 69 73 type type NN 74 75 1 1 CD 76 79 and and CC 80 89 mechanism mechanism NN 90 92 of of IN 93 108 down-regulation down-regulation NN 109 111 of of IN 112 118 ICAM-1 icam-1 NN 119 121 or or CC 122 127 LFA-1 lfa-1 NN 128 130 in in IN 131 152 adult-T-cell-leukemia adult-t-cell-leukemia JJ 153 157 cell cell NN 158 163 lines line NNS 163 164 . . . 166 169 The the DT 170 177 present present JJ 178 183 study study NN 184 187 was be VBD 188 198 undertaken undertake VBN 199 201 to to TO 202 211 determine determine VB 212 215 the the DT 216 220 role role NN 221 223 of of IN 224 230 HTLV-I HTLV-I NNP 231 235 TaxI TaxI NNP 236 238 in in IN 239 242 the the DT 243 256 up-regulation up-regulation NN 257 259 of of IN 260 266 ICAM-I ICAM-I NNP 267 270 and and CC 271 276 LFA-3 LFA-3 NNP 277 279 in in IN 280 285 human human JJ 286 287 T t NN 288 293 cells cell NNS 294 305 transformed transform VBN 306 310 with with IN 311 317 HTLV-I HTLV-I NNP 318 321 and and CC 322 325 the the DT 326 335 mechanism mechanism NN 336 338 of of IN 339 354 down-regulation down-regulation NN 355 357 of of IN 358 364 ICAM-I ICAM-I NNP 365 368 and and CC 369 374 LFA-I LFA-I NNP 375 377 in in IN 378 389 ATL-derived atl-derived JJ 390 394 cell cell NN 395 400 lines line NNS 400 401 . . . 402 411 Induction Induction NNP 412 414 of of IN 415 419 TaxI TaxI NNP 420 422 in in IN 423 424 a a DT 425 430 human human JJ 431 437 T-cell t-cell NN 438 442 line line NN 443 449 Jurkat Jurkat NNP 450 458 carrying carry VBG 459 462 the the DT 463 467 TaxI TaxI NNP 468 472 gene gene NN 473 478 under under IN 479 482 the the DT 483 498 metallothionein metallothionein NN 499 507 promoter promoter NN 508 511 led lead VBD 512 514 to to TO 515 524 increases increase NNS 525 527 in in IN 528 532 mRNA mRNA NNP 533 536 and and CC 537 544 surface surface NN 545 555 expression expression NN 556 558 of of IN 559 565 ICAM-I ICAM-I NNP 565 566 . . . 567 570 The the DT 571 579 response response NN 580 582 of of IN 583 588 LFA-3 LFA-3 NNP 589 591 to to TO 592 596 TaxI TaxI NNP 597 606 induction induction NN 607 610 was be VBD 610 611 , , , 612 614 on on IN 615 618 the the DT 619 624 other other JJ 625 629 hand hand NN 629 630 , , , 631 641 relatively relatively RB 642 646 slow slow JJ 647 650 and and CC 651 655 weak weak JJ 655 656 , , , 657 660 and and CC 661 666 might might MD 667 669 be be VB 670 678 indirect indirect JJ 678 679 . . . 680 695 Transactivation Transactivation NNP 696 698 of of IN 699 702 the the DT 703 709 ICAM-I ICAM-I NNP 710 718 promoter promoter NN 719 721 by by IN 722 726 TaxI TaxI NNP 727 730 was be VBD 731 738 further further RBR 739 744 shown show VBN 745 747 by by IN 748 763 co-transfection co-transfection NN 764 766 of of IN 767 768 a a DT 769 772 CAT CAT NNP 773 781 reporter reporter NN 782 791 construct construct NN 792 796 with with IN 797 800 the the DT 801 807 ICAM-I ICAM-I NNP 808 816 promoter promoter NN 817 820 and and CC 821 822 a a DT 823 830 plasmid plasmid NN 831 841 expressing express VBG 842 846 TaxI TaxI NNP 846 847 . . . 848 851 The the DT 852 861 mechanism mechanism NN 862 864 of of IN 865 880 down-regulation down-regulation NN 881 883 of of IN 884 890 ICAM-I ICAM-I NNP 891 893 or or CC 894 899 LFA-I LFA-I NNP 900 902 in in IN 903 904 4 4 CD 905 908 ATL ATL NNP 909 913 cell cell NN 914 919 lines line NNS 920 923 was be VBD 924 928 next next JJ 929 937 examined examine VBN 937 938 . . . 939 945 ICAM-I ICAM-I NNP 946 950 mRNA mRNA NNP 951 954 was be VBD 955 960 quite quite RB 961 964 low low JJ 965 967 in in IN 968 972 MT-I MT-I NNP 972 973 , , , 974 977 but but CC 978 980 no no DT 981 988 genomic genomic JJ 989 996 changes change NNS 997 1001 were be VBD 1002 1007 found find VBN 1007 1008 . . . 1009 1012 The the DT 1013 1016 CAT CAT NNP 1017 1025 reporter reporter NN 1026 1030 with with IN 1031 1034 the the DT 1035 1041 ICAM-I ICAM-I NNP 1042 1050 promoter promoter NN 1051 1054 was be VBD 1055 1063 inactive inactive JJ 1064 1066 in in IN 1067 1071 MT-I MT-I NNP 1071 1072 . . . 1073 1080 Finally finally RB 1080 1081 , , , 1082 1090 combined combined JJ 1091 1100 treatment treatment NN 1101 1103 of of IN 1104 1108 MT-I MT-I NNP 1109 1113 with with IN 1114 1127 5-azacytidine 5-azacytidine NN 1128 1131 and and CC 1132 1141 IFN-gamma IFN-gamma NNP 1142 1149 induced induce VBD 1150 1163 re-expression re-expression NN 1164 1166 of of IN 1167 1173 ICAM-I ICAM-I NNP 1173 1174 . . . 1175 1187 Collectively collectively RB 1187 1188 , , , 1189 1190 ( ( ( 1190 1191 a a DT 1191 1192 ) ) ) 1193 1208 transcriptional transcriptional JJ 1209 1218 factor(s) factor(s) NN 1219 1228 necessary necessary JJ 1229 1232 for for IN 1233 1243 expression expression NN 1244 1246 of of IN 1247 1253 ICAM-I ICAM-I NNP 1254 1258 gene gene NN 1259 1262 may may MD 1263 1265 be be VB 1266 1275 repressed repress VBN 1276 1278 in in IN 1279 1283 MT-I MT-I NNP 1284 1291 through through IN 1292 1295 DNA DNA NNP 1296 1307 methylation methylation NN 1307 1308 . . . 1309 1314 Three three CD 1315 1320 other other JJ 1321 1324 ATL ATL NNP 1325 1329 cell cell NN 1330 1335 lines line NNS 1336 1337 ( ( ( 1337 1343 TL-OmI TL-OmI NNP 1343 1344 , , , 1345 1349 H582 H582 NNP 1349 1350 , , , 1351 1357 HuT102 HuT102 NNP 1357 1358 ) ) ) 1359 1363 were be VBD 1364 1369 found find VBN 1370 1372 to to TO 1373 1377 have have VB 1378 1384 little little JJ 1385 1389 mRNA mrna NN 1390 1393 for for IN 1394 1397 the the DT 1398 1403 LFA-I LFA-I NNP 1404 1408 beta beta NN 1409 1414 chain chain NN 1415 1416 ( ( ( 1416 1420 CD18 cd18 NN 1420 1421 ) ) ) 1421 1422 . . . 1423 1427 H582 H582 NNP 1428 1431 and and CC 1432 1438 HuT102 HuT102 NNP 1439 1443 were be VBD 1444 1448 also also RB 1449 1457 negative negative JJ 1458 1461 for for IN 1462 1465 the the DT 1466 1471 LFA-I LFA-I NNP 1472 1477 alpha alpha NN 1478 1483 chain chain NN 1484 1485 ( ( ( 1485 1490 CDIIa CDIIa NNP 1490 1491 ) ) ) 1492 1496 mRNA mRNA NNP 1496 1497 . . . 1498 1500 No no DT 1501 1508 genomic genomic JJ 1509 1516 changes change NNS 1517 1521 were be VBD 1522 1527 found find VBN 1527 1528 , , , 1529 1532 and and CC 1533 1534 a a DT 1535 1538 CAT CAT NNP 1539 1547 reporter reporter NN 1548 1552 gene gene NN 1553 1557 with with IN 1558 1561 the the DT 1562 1566 CD18 cd18 NN 1567 1575 promoter promoter NN 1576 1579 was be VBD 1580 1588 inactive inactive JJ 1589 1591 in in IN 1592 1595 the the DT 1596 1597 3 3 CD 1598 1600 of of IN 1601 1605 them them PRP 1605 1606 , , , 1607 1612 again again RB 1613 1623 suggesting suggest VBG 1624 1628 lack lack NN 1629 1631 of of IN 1632 1633 ( ( ( 1633 1634 a a DT 1634 1635 ) ) ) 1636 1651 transcriptional transcriptional JJ 1652 1661 factor(s) factor(s) NN 1662 1671 necessary necessary JJ 1672 1675 for for IN 1676 1680 CD18 cd18 NN 1681 1691 expression expression NN 1691 1692 . . .