0 3 The the DT 4 9 human human JJ 10 15 TCF-1 tcf-1 NN 16 20 gene gene NN 21 28 encodes encode VBZ 29 30 a a DT 31 38 nuclear nuclear JJ 39 50 DNA-binding dna-binding JJ 51 58 protein protein NN 59 67 uniquely uniquely RB 68 77 expressed express VBN 78 80 in in IN 81 87 normal normal JJ 88 91 and and CC 92 102 neoplastic neoplastic JJ 103 112 T-lineage t-lineage NN 113 124 lymphocytes lymphocyte NNS 124 125 . . . 127 130 The the DT 131 136 TCF-1 tcf-1 NN 137 141 gene gene NN 142 149 encodes encode VBZ 150 151 a a DT 152 160 putative putative JJ 161 174 transcription transcription NN 175 181 factor factor NN 182 186 with with IN 187 195 affinity affinity NN 196 199 for for IN 200 201 a a DT 202 210 sequence sequence NN 211 216 motif motif NN 217 226 occurring occur VBG 227 229 in in IN 230 231 a a DT 232 238 number number NN 239 241 of of IN 242 248 T-cell t-cell NN 249 258 enhancers enhancer NNS 258 259 . . . 260 265 TCF-1 TCF-1 NNP 266 270 mRNA mRNA NNP 271 274 was be VBD 275 285 originally originally RB 286 291 found find VBN 292 294 to to TO 295 297 be be VB 298 307 expressed express VBN 308 310 in in IN 311 312 a a DT 313 314 T t NN 315 328 cell-specific cell-specific JJ 329 336 fashion fashion NN 337 343 within within IN 344 345 a a DT 346 349 set set NN 350 352 of of IN 353 358 human human JJ 359 362 and and CC 363 368 mouse mouse NN 369 373 cell cell NN 374 379 lines line NNS 379 380 . . . 381 383 In in IN 384 392 contrast contrast NN 392 393 , , , 394 404 expression expression NN 405 415 reportedly reportedly RB 416 422 occurs occur VBZ 423 425 in in IN 426 434 multiple multiple JJ 435 446 nonlymphoid nonlymphoid JJ 447 454 tissues tissue NNS 455 461 during during IN 462 468 murine murine JJ 469 482 embryogenesis embryogenesis NN 482 483 . . . 484 486 We we PRP 487 491 have have VBP 492 495 now now RB 496 502 raised raise VBN 503 504 a a DT 505 515 monoclonal monoclonal JJ 516 524 antibody antibody NN 525 527 to to TO 528 536 document document NN 537 547 expression expression NN 548 551 and and CC 552 564 biochemistry biochemistry NN 565 567 of of IN 568 571 the the DT 572 577 human human JJ 578 583 TCF-1 tcf-1 NN 584 591 protein protein NN 591 592 . . . 593 595 As as IN 596 604 expected expect VBN 604 605 , , , 606 609 the the DT 610 615 TCF-1 tcf-1 NN 616 623 protein protein NN 624 627 was be VBD 628 638 detectable detectable JJ 639 643 only only RB 644 646 in in IN 647 651 cell cell NN 652 657 lines line NNS 658 660 of of IN 661 662 T t NN 663 670 lineage lineage NN 670 671 . . . 672 675 Its its PRP$ 676 686 expression expression NN 687 690 was be VBD 691 697 always always RB 698 708 restricted restricted JJ 709 711 to to TO 712 715 the the DT 716 723 nucleus nucleus NN 723 724 . . . 725 745 Immunohistochemistry Immunohistochemistry NNP 746 748 on on IN 749 750 a a DT 751 756 panel panel NN 757 759 of of IN 760 765 human human JJ 766 773 tissues tissue NNS 774 782 revealed reveal VBD 783 787 that that IN 788 791 the the DT 792 797 TCF-1 tcf-1 NN 798 805 protein protein NN 806 809 was be VBD 810 815 found find VBN 816 827 exclusively exclusively RB 828 830 in in IN 831 841 thymocytes thymocyte NNS 842 845 and and CC 846 848 in in IN 849 853 CD3+ cd3+ JJ 854 855 T t NN 856 861 cells cell NNS 862 864 in in IN 865 875 peripheral peripheral JJ 876 884 lymphoid lymphoid JJ 885 892 tissues tissue NNS 892 893 . . . 894 901 Western Western NNP 902 910 blotting blotting NN 911 918 yielded yield VBD 919 920 a a DT 921 924 set set NN 925 927 of of IN 928 933 bands band NNS 934 941 ranging range VBG 942 946 from from IN 947 949 25 25 CD 950 952 kD kd NN 953 955 to to TO 956 958 55 55 CD 959 961 kD kd NN 961 962 , , , 963 972 resulting result VBG 973 977 from from IN 978 987 extensive extensive JJ 988 999 alternative alternative JJ 1000 1008 splicing splicing NN 1008 1009 . . . 1010 1013 The the DT 1014 1019 TCF-1 tcf-1 NN 1020 1027 protein protein NN 1028 1031 was be VBD 1032 1042 detectable detectable JJ 1043 1045 in in IN 1046 1049 all all DT 1050 1057 samples sample NNS 1058 1060 of of IN 1061 1062 a a DT 1063 1066 set set NN 1067 1069 of of IN 1070 1072 22 22 CD 1073 1079 T-cell t-cell NN 1080 1092 malignancies malignancy NNS 1093 1095 of of IN 1096 1103 various various JJ 1104 1110 stages stage NNS 1111 1113 of of IN 1114 1124 maturation maturation NN 1124 1125 , , , 1126 1129 but but CC 1130 1133 was be VBD 1134 1140 absent absent JJ 1141 1145 from from IN 1146 1147 a a DT 1148 1153 large large JJ 1154 1160 number number NN 1161 1163 of of IN 1164 1169 other other JJ 1170 1181 hematologic hematologic JJ 1182 1191 neoplasms neoplasm NNS 1191 1192 . . . 1193 1198 These these DT 1199 1211 observations observation NNS 1212 1217 imply imply VBP 1218 1219 a a DT 1220 1221 T t NN 1222 1235 cell-specific cell-specific JJ 1236 1244 function function NN 1245 1248 for for IN 1249 1254 TCF-1 TCF-1 NNP 1254 1255 , , , 1256 1257 a a DT 1258 1264 notion notion NN 1265 1277 corroborated corroborate VBN 1278 1280 by by IN 1281 1287 recent recent JJ 1288 1300 observations observation NNS 1301 1303 on on IN 1304 1309 Tcf-1 tcf-1 NN 1310 1319 knock-out knock-out JJ 1320 1324 mice mouse NNS 1324 1325 . . . 1326 1328 In in IN 1329 1337 addition addition NN 1337 1338 , , , 1339 1344 these these DT 1345 1352 results result NNS 1353 1361 indicate indicate VBP 1362 1366 that that IN 1367 1374 nuclear nuclear JJ 1375 1380 TCF-1 tcf-1 NN 1381 1391 expression expression NN 1392 1395 can can MD 1396 1401 serve serve VB 1402 1404 as as IN 1405 1406 a a DT 1407 1420 pan-T-lineage pan-t-lineage NN 1421 1427 marker marker NN 1428 1430 in in IN 1431 1434 the the DT 1435 1444 diagnosis diagnosis NN 1445 1447 of of IN 1448 1456 lymphoid lymphoid JJ 1457 1469 malignancies malignancy NNS 1469 1470 . . .