0 12 Constitutive constitutive JJ 13 27 overexpression overexpression NN 28 30 of of IN 31 34 the the DT 35 45 L-selectin L-selectin NNP 46 50 gene gene NN 51 53 in in IN 54 59 fresh fresh JJ 60 68 leukemic leukemic JJ 69 74 cells cell NNS 75 77 of of IN 78 83 adult adult JJ 84 90 T-cell t-cell NN 91 99 leukemia leukemia NN 100 104 that that WDT 105 108 can can MD 109 111 be be VB 112 126 transactivated transactivate VBN 127 129 by by IN 130 135 human human JJ 136 142 T-cell t-cell NN 143 155 lymphotropic lymphotropic JJ 156 161 virus virus NN 162 166 type type NN 167 168 1 1 CD 169 172 Tax tax NN 172 173 . . . 175 185 L-selectin L-selectin NNP 186 188 is be VBZ 189 191 an an DT 192 200 adhesion adhesion NN 201 209 molecule molecule NN 210 212 of of IN 213 216 the the DT 217 225 selectin selectin NN 226 232 family family NN 233 237 that that WDT 238 246 mediates mediate VBZ 247 250 the the DT 251 258 initial initial JJ 259 263 step step NN 264 266 of of IN 267 276 leukocyte leukocyte NN 277 285 adhesion adhesion NN 286 288 to to TO 289 297 vascular vascular JJ 298 309 endothelium endothelium NN 309 310 . . . 311 315 Upon upon IN 316 324 cellular cellular JJ 325 335 activation activation NN 335 336 , , , 337 347 expression expression NN 348 350 of of IN 351 354 the the DT 355 365 L-selectin L-selectin NNP 366 370 gene gene NN 371 373 is be VBZ 374 387 downregulated downregulate VBN 388 390 at at IN 391 395 both both CC 396 399 the the DT 400 407 protein protein NN 408 411 and and CC 412 416 mRNA mrna NN 417 423 levels level NNS 423 424 . . . 425 427 To to TO 428 438 understand understand VB 439 442 the the DT 443 452 mechanism mechanism NN 453 455 of of IN 456 464 leukemic leukemic JJ 465 469 cell cell NN 470 482 infiltration infiltration NN 483 487 into into IN 488 494 organs organ NNS 494 495 , , , 496 498 we we PRP 499 506 studied study VBD 507 510 the the DT 511 521 expression expression NN 522 525 and and CC 526 536 regulation regulation NN 537 539 of of IN 540 550 L-selectin L-selectin NNP 551 555 mRNA mRNA NNP 556 558 in in IN 559 564 fresh fresh JJ 565 573 leukemic leukemic JJ 574 579 cells cell NNS 580 582 of of IN 583 588 adult adult JJ 589 595 T-cell t-cell NN 596 604 leukemia leukemia NN 605 606 ( ( ( 606 609 ATL ATL NNP 609 610 ) ) ) 611 619 patients patient NNS 620 623 and and CC 624 636 investigated investigate VBD 637 640 the the DT 641 649 response response NN 650 652 of of IN 653 656 the the DT 657 667 L-selectin L-selectin NNP 668 676 promoter promoter NN 677 679 to to TO 680 685 human human JJ 686 692 T-cell t-cell NN 693 705 lymphotropic lymphotropic JJ 706 711 virus virus NN 712 716 type type NN 717 718 1 1 CD 719 720 ( ( ( 720 726 HTLV-1 HTLV-1 NNP 726 727 ) ) ) 728 731 Tax Tax NNP 731 732 , , , 733 738 which which WDT 739 741 is be VBZ 742 743 a a DT 744 749 viral viral JJ 750 765 transcriptional transcriptional JJ 766 780 transactivator transactivator NN 780 781 . . . 782 786 Flow Flow NNP 787 796 cytometry cytometry NN 797 803 showed show VBD 804 808 that that IN 809 819 L-selectin L-selectin NNP 820 823 was be VBD 824 833 expressed express VBN 834 836 on on IN 837 842 fresh fresh JJ 843 846 ATL ATL NNP 847 852 cells cell NNS 853 858 along along IN 859 863 with with IN 864 869 other other JJ 870 880 activation activation NN 881 889 antigens antigen NNS 889 890 . . . 891 899 Northern Northern NNP 900 904 blot blot NN 905 913 analysis analysis NN 914 920 showed show VBD 921 925 that that IN 926 929 ATL ATL NNP 930 935 cells cell NNS 936 949 overexpressed overexpresse VBD 950 954 that that IN 955 965 L-selectin L-selectin NNP 966 970 mRNA mRNA NNP 971 974 and and CC 975 979 that that IN 980 983 the the DT 984 989 level level NN 990 993 was be VBD 994 1004 aberrantly aberrantly RB 1005 1016 upregulated upregulate VBN 1017 1022 after after IN 1023 1026 PMA PMA NNP 1027 1038 stimulation stimulation NN 1038 1039 . . . 1040 1047 Studies study NNS 1048 1053 using use VBG 1054 1056 in in FW 1057 1061 situ situ FW 1062 1075 hybridization hybridization NN 1076 1082 showed show VBD 1083 1093 expression expression NN 1094 1096 of of IN 1097 1100 the the DT 1101 1111 L-selectin L-selectin NNP 1112 1116 mRNA mRNA NNP 1117 1119 in in IN 1120 1123 the the DT 1124 1136 infiltrating infiltrate VBG 1137 1145 leukemic leukemic JJ 1146 1151 cells cell NNS 1152 1154 in in IN 1155 1158 the the DT 1159 1164 liver liver NN 1165 1167 of of IN 1168 1171 two two CD 1172 1175 ATL ATL NNP 1176 1184 patients patient NNS 1184 1185 . . . 1186 1197 Intravenous intravenous JJ 1198 1207 injection injection NN 1208 1210 of of IN 1211 1212 a a DT 1213 1216 rat rat NN 1217 1223 T-cell t-cell NN 1224 1228 line line NN 1229 1233 that that WDT 1234 1247 overexpresses overexpresse VBZ 1248 1258 L-selectin L-selectin NNP 1259 1265 showed show VBD 1266 1275 increased increase VBN 1276 1281 organ organ NN 1282 1294 infiltration infiltration NN 1294 1295 . . . 1296 1299 The the DT 1300 1309 induction induction NN 1310 1312 of of IN 1313 1316 Tax Tax NNP 1317 1327 expression expression NN 1328 1330 in in IN 1331 1335 JPX9 JPX9 NNP 1336 1341 cells cell NNS 1342 1350 resulted result VBD 1351 1353 in in IN 1354 1359 about about RB 1360 1361 a a DT 1362 1369 twofold twofold JJ 1370 1378 increase increase NN 1379 1381 in in IN 1382 1385 the the DT 1386 1390 mRNA mrna NN 1391 1401 expression expression NN 1402 1408 levels level NNS 1409 1417 compared compare VBN 1418 1422 with with IN 1423 1426 the the DT 1427 1432 basal basal JJ 1433 1438 level level NN 1438 1439 . . . 1440 1455 Chloramphenicol Chloramphenicol NNP 1456 1473 acetyltransferase acetyltransferase NNP 1474 1475 ( ( ( 1475 1478 CAT CAT NNP 1478 1479 ) ) ) 1480 1485 assay assay NN 1486 1491 after after IN 1492 1501 transient transient JJ 1502 1516 cotransfection cotransfection NN 1517 1523 showed show VBD 1524 1529 about about IN 1530 1531 a a DT 1532 1540 fivefold fivefold JJ 1541 1556 transactivation transactivation NN 1557 1559 of of IN 1560 1563 the the DT 1564 1574 L-selectin L-selectin NNP 1575 1583 promoter promoter NN 1584 1586 by by IN 1587 1590 Tax Tax NNP 1590 1591 . . . 1592 1595 The the DT 1596 1601 serum serum NN 1602 1607 level level NN 1608 1610 of of IN 1611 1614 the the DT 1615 1619 shed shed JJ 1620 1624 form form NN 1625 1627 of of IN 1628 1638 L-selectin L-selectin NNP 1639 1642 was be VBD 1643 1656 significantly significantly RB 1657 1666 increased increase VBN 1667 1669 in in IN 1670 1673 ATL ATL NNP 1674 1682 patients patient NNS 1683 1684 ( ( ( 1684 1688 mean mean NN 1689 1692 +/- +/- SYM 1693 1695 SD SD NNP 1695 1696 , , , 1697 1704 4,215.4 4,215.4 CD 1705 1708 +/- +/- SYM 1709 1714 4,111 4,111 CD 1715 1720 ng/mL ng/ml NN 1720 1721 ) ) ) 1722 1730 compared compare VBN 1731 1735 with with IN 1736 1741 those those DT 1742 1744 of of IN 1745 1757 asymptomatic asymptomatic JJ 1758 1766 carriers carrier NNS 1767 1770 and and CC 1771 1778 healthy healthy JJ 1779 1784 blood blood NN 1785 1791 donors donor NNS 1792 1793 ( ( ( 1793 1797 mean mean NN 1798 1801 +/- +/- SYM 1802 1804 SD SD NNP 1804 1805 , , , 1806 1813 1,148.0 1,148.0 CD 1814 1817 +/- +/- SYM 1818 1823 269.0 269.0 CD 1824 1829 ng/mL ng/ml NN 1830 1833 and and CC 1834 1839 991.9 991.9 CD 1840 1843 +/- +/- SYM 1844 1847 224 224 CD 1848 1853 ng/mL ng/ml NN 1853 1854 , , , 1855 1867 respectively respectively RB 1867 1868 ) ) ) 1868 1869 . . . 1870 1875 These these DT 1876 1883 results result NNS 1884 1893 indicated indicate VBD 1894 1898 that that IN 1899 1902 ATL ATL NNP 1903 1908 cells cell NNS 1909 1923 constitutively constitutively RB 1924 1935 overexpress overexpres VBP 1936 1939 the the DT 1940 1950 L-selectin L-selectin NNP 1951 1955 gene gene NN 1956 1960 that that WDT 1961 1964 can can MD 1965 1967 be be VB 1968 1982 transactivated transactivate VBN 1983 1985 by by IN 1986 1992 HTLV-1 HTLV-1 NNP 1993 1996 Tax Tax NNP 1996 1997 . . . 1998 2001 The the DT 2002 2016 overexpression overexpression NN 2017 2019 of of IN 2020 2030 L-selectin L-selectin NNP 2030 2031 , , , 2032 2034 as as RB 2035 2039 well well RB 2040 2042 as as IN 2043 2045 of of IN 2046 2058 inflammatory inflammatory JJ 2059 2068 cytokines cytokine NNS 2068 2069 , , , 2070 2072 by by IN 2073 2076 ATL ATL NNP 2077 2082 cells cell NNS 2083 2086 may may MD 2087 2094 provide provide VB 2095 2096 a a DT 2097 2102 basis basis NN 2103 2106 for for IN 2107 2110 ATL ATL NNP 2111 2116 cells cell NNS 2117 2119 to to TO 2120 2126 attach attach VB 2127 2130 the the DT 2131 2139 vascular vascular JJ 2140 2151 endothelium endothelium NN 2151 2152 , , , 2153 2160 leading lead VBG 2161 2163 to to TO 2164 2178 transmigration transmigration NN 2179 2182 and and CC 2183 2188 organ organ NN 2189 2200 infitration infitration NN 2200 2201 . . .