0 14 Identification identification NN 15 17 of of IN 18 27 essential essential JJ 28 32 GATA GATA NNP 33 36 and and CC 37 40 Ets Ets NNP 41 48 binding binding NN 49 55 motifs motif NNS 56 62 within within IN 63 66 the the DT 67 75 promoter promoter NN 76 78 of of IN 79 82 the the DT 83 91 platelet platelet NN 92 104 glycoprotein glycoprotein NN 105 107 Ib Ib NNP 108 113 alpha alpha NN 114 118 gene gene NN 118 119 . . . 121 129 Platelet Platelet NNP 130 142 glycoprotein glycoprotein NN 143 144 ( ( ( 144 146 GP GP NNP 146 147 ) ) ) 148 155 Ib-IX-V Ib-IX-V NNP 156 158 is be VBZ 159 160 a a DT 161 173 multisubunit multisubunit JJ 174 182 adhesion adhesion NN 183 191 receptor receptor NN 192 196 that that WDT 197 205 supports support VBZ 206 214 platelet platelet NN 215 225 attachment attachment NN 226 228 to to TO 229 241 thrombogenic thrombogenic JJ 242 250 surfaces surface NNS 251 253 at at IN 254 259 sites site NNS 260 262 of of IN 263 271 vascular vascular JJ 272 278 injury injury NN 278 279 . . . 280 283 The the DT 284 294 congenital congenital JJ 295 302 absence absence NN 303 305 of of IN 306 309 the the DT 310 318 receptor receptor NN 319 326 results result VBZ 327 329 in in IN 330 331 a a DT 332 340 bleeding bleeding JJ 341 349 disorder disorder NN 350 360 associated associate VBN 361 365 with with IN 366 367 “ `` `` 367 372 giant giant JJ 372 373 ” '' '' 374 383 platelets platelet NNS 383 384 , , , 385 386 a a DT 387 396 condition condition NN 397 404 linking link VBG 405 408 the the DT 409 419 expression expression NN 420 422 of of IN 423 426 the the DT 427 434 complex complex NN 435 437 to to TO 438 446 platelet platelet NN 447 460 morphogenesis morphogenesis NN 460 461 . . . 462 464 To to TO 465 475 understand understand VB 476 482 better well RBR 483 486 the the DT 487 497 expression expression NN 498 500 of of IN 501 504 the the DT 505 507 GP GP NNP 508 515 Ib-IX-V Ib-IX-V NNP 516 523 complex complex NN 523 524 , , , 525 532 studies study NNS 533 537 were be VBD 538 548 undertaken undertake VBN 549 551 to to TO 552 558 define define VB 559 562 the the DT 563 572 essential essential JJ 573 580 genetic genetic JJ 581 589 elements element NNS 590 600 supporting support VBG 601 604 the the DT 605 615 expression expression NN 616 618 of of IN 619 622 the the DT 623 636 alpha-subunit alpha-subunit NN 637 639 of of IN 640 643 the the DT 644 651 complex complex NN 652 653 ( ( ( 653 655 GP GP NNP 656 658 Ib Ib NNP 659 664 alpha alpha NN 664 665 ) ) ) 665 666 . . . 667 669 GP GP NNP 670 672 Ib Ib NNP 673 678 alpha alpha NN 679 687 promoter promoter NN 688 696 activity activity NN 697 700 was be VBD 701 710 evaluated evaluate VBN 711 713 by by IN 714 726 transfection transfection NN 727 729 of of IN 730 735 human human JJ 736 751 erythroleukemia erythroleukemia NN 752 757 cells cell NNS 758 762 with with IN 763 771 reporter reporter NN 772 780 plasmids plasmid NNS 781 787 coding cod VBG 788 791 for for IN 792 795 the the DT 796 802 enzyme enzyme NN 802 803 , , , 804 814 luciferase luciferase NN 814 815 . . . 816 823 Studies study NNS 824 828 were be VBD 829 838 initiated initiate VBN 839 843 with with IN 844 845 a a DT 846 854 fragment fragment NN 855 864 extending extend VBG 865 870 2,738 2,738 CD 871 882 nucleotides nucleotide NNS 883 884 5 5 CD 884 885 ’ ' SYM 886 888 to to TO 889 892 the the DT 893 906 transcription transcription NN 907 912 start start NN 913 917 site site NN 918 921 and and CC 922 926 lead lead VBP 927 929 to to TO 930 933 the the DT 934 948 identification identification NN 949 951 of of IN 952 955 253 253 CD 956 967 nucleotides nucleotide NNS 968 977 retaining retain VBG 978 982 full full JJ 983 991 promoter promoter NN 992 1000 activity activity NN 1001 1003 in in IN 1004 1009 human human JJ 1010 1025 erythroleukemia erythroleukemia NN 1026 1031 cells cell NNS 1031 1032 . . . 1033 1035 In in IN 1036 1041 cells cell NNS 1042 1044 of of IN 1045 1061 nonhematopoietic nonhematopoietic JJ 1062 1069 lineage lineage NN 1069 1070 , , , 1071 1076 human human JJ 1077 1088 endothelial endothelial JJ 1089 1092 and and CC 1093 1097 HeLa HeLa NNP 1098 1103 cells cell NNS 1103 1104 , , , 1105 1108 the the DT 1109 1111 GP GP NNP 1112 1114 Ib Ib NNP 1115 1120 alpha alpha NN 1121 1129 promoter promoter NN 1130 1138 activity activity NN 1139 1142 was be VBD 1143 1145 no no RB 1146 1153 greater great JJR 1154 1158 than than IN 1159 1169 background background NN 1170 1176 levels level NNS 1177 1185 obtained obtain VBN 1186 1190 with with IN 1191 1203 promoterless promoterless JJ 1204 1214 constructs construct NNS 1214 1215 . . . 1216 1219 Gel Gel NNP 1220 1225 shift shift NN 1226 1232 assays assay NNS 1233 1236 and and CC 1237 1250 site-directed site-directed JJ 1251 1262 mutagenesis mutagenesis NN 1263 1270 studies study NNS 1271 1278 defined define VBD 1279 1288 essential essential JJ 1289 1293 GATA GATA NNP 1294 1297 and and CC 1298 1301 Ets Ets NNP 1302 1309 binding binding NN 1310 1316 motifs motif NNS 1317 1319 93 93 CD 1320 1323 and and CC 1324 1327 150 150 CD 1328 1339 nucleotides nucleotide NNS 1340 1348 upstream upstream RB 1349 1351 of of IN 1352 1355 the the DT 1356 1369 transcription transcription NN 1370 1375 start start NN 1376 1380 site site NN 1380 1381 , , , 1382 1383 a a DT 1384 1391 finding finding NN 1392 1397 which which WDT 1398 1405 further further RBR 1406 1419 substantiates substantiate VBZ 1420 1425 these these DT 1426 1434 elements element NNS 1435 1437 as as IN 1438 1447 important important JJ 1448 1460 determinants determinant NNS 1461 1463 of of IN 1464 1478 megakaryocytic megakaryocytic JJ 1479 1483 gene gene NN 1484 1494 expression expression NN 1494 1495 . . . 1496 1499 The the DT 1500 1507 results result NNS 1508 1514 define define VBP 1515 1524 essential essential JJ 1525 1535 cis-acting cis-acting JJ 1536 1544 elements element NNS 1545 1556 responsible responsible JJ 1557 1560 for for IN 1561 1564 the the DT 1565 1575 expression expression NN 1576 1578 of of IN 1579 1581 GP GP NNP 1582 1584 Ib Ib NNP 1585 1590 alpha alpha NN 1591 1594 and and CC 1595 1602 provide provide VBP 1603 1611 insights insight NNS 1612 1616 into into IN 1617 1626 molecular molecular JJ 1627 1633 events event NNS 1634 1644 coinciding coincide VBG 1645 1649 with with IN 1650 1653 the the DT 1654 1661 release release NN 1662 1664 of of IN 1665 1671 normal normal JJ 1672 1681 platelets platelet NNS 1682 1686 into into IN 1687 1690 the the DT 1691 1702 bloodstream bloodstream NN 1702 1703 . . .