0 3 The the DT 4 14 activation activation NN 15 17 of of IN 18 21 the the DT 22 30 Jak-STAT jak-stat JJ 31 32 1 1 CD 33 42 signaling signaling NN 43 50 pathway pathway NN 51 53 by by IN 54 58 IL-5 il-5 NN 59 61 in in IN 62 73 eosinophils eosinophil NNS 73 74 . . . 76 79 The the DT 80 93 intracellular intracellular JJ 94 100 signal signal NN 101 113 transduction transduction NN 114 116 of of IN 117 121 IL-5 il-5 NN 122 124 in in IN 125 136 eosinophils eosinophil NNS 137 139 is be VBZ 140 147 unknown unknown JJ 147 148 . . . 149 152 The the DT 153 162 objective objective NN 163 165 of of IN 166 170 this this DT 171 176 study study NN 177 180 was be VBD 181 183 to to TO 184 195 investigate investigate VB 196 199 the the DT 200 211 involvement involvement NN 212 214 of of IN 215 218 the the DT 219 224 newly newly RB 225 235 discovered discover VBN 236 244 Jak-STAT jak-stat JJ 245 252 pathway pathway NN 253 255 in in IN 256 259 the the DT 260 264 IL-5 IL-5 NNP 265 271 signal signal NN 272 284 transduction transduction NN 285 294 mechanism mechanism NN 294 295 . . . 296 307 Eosinophils Eosinophil NNP 308 312 were be VBD 313 321 purified purify VBN 322 326 from from IN 327 337 peripheral peripheral JJ 338 343 blood blood NN 344 346 by by IN 347 360 discontinuous discontinuous JJ 361 368 Percoll Percoll NNP 369 378 gradients gradient NNS 379 382 and and CC 383 393 stimulated stimulate VBN 394 398 with with IN 399 403 IL-5 IL-5 NNP 403 404 . . . 405 408 The the DT 409 420 involvement involvement NN 421 423 of of IN 424 427 Jak Jak NNP 428 429 2 2 CD 430 433 was be VBD 434 446 investigated investigate VBN 447 449 by by IN 450 469 immunoprecipitation immunoprecipitation NN 470 478 followed follow VBN 479 481 by by IN 482 496 immunoblotting immunoblotte VBG 497 500 for for IN 501 509 tyrosine tyrosine NN 510 525 phosphorylation phosphorylation NN 525 526 . . . 527 530 The the DT 531 541 activation activation NN 542 544 of of IN 545 548 Jak Jak NNP 549 550 2 2 CD 551 554 was be VBD 555 562 studied study VBN 563 565 by by IN 566 585 autophosphorylation autophosphorylation NN 586 588 of of IN 589 592 the the DT 593 611 immunoprecipitated immunoprecipitated JJ 612 618 kinase kinase NNP 618 619 . . . 620 623 Jak Jak NNP 624 625 2 2 CD 626 629 was be VBD 630 638 tyrosine tyrosine NN 639 653 phosphorylated phosphorylated JJ 654 660 within within IN 661 662 1 1 CD 663 665 to to TO 666 667 3 3 CD 668 671 min min NN 672 677 after after IN 678 689 stimulation stimulation NN 690 692 of of IN 693 704 eosinophils eosinophil NNS 705 709 with with IN 710 714 IL-5 IL-5 NNP 714 715 . . . 716 723 Further further RB 723 724 , , , 725 728 the the DT 729 747 immunoprecipitated immunoprecipitate VBN 748 751 Jak Jak NNP 752 753 2 2 CD 754 762 obtained obtain VBN 763 767 from from IN 768 783 IL-5-stimulated il-5-stimulated JJ 784 789 cells cell NNS 790 799 underwent undergo VBD 800 819 autophosphorylation autophosphorylation NN 819 820 . . . 821 824 Jak Jak NNP 825 826 2 2 CD 827 841 coprecipitated coprecipitate VBD 842 846 with with IN 847 850 the the DT 851 863 beta-subunit beta-subunit NN 864 866 of of IN 867 870 the the DT 871 875 IL-5 IL-5 NNP 876 884 receptor receptor NN 884 885 , , , 886 896 suggesting suggest VBG 897 898 a a DT 899 907 physical physical JJ 908 919 association association NN 920 922 of of IN 923 926 the the DT 927 933 kinase kinase NNP 934 938 with with IN 939 942 the the DT 943 951 receptor receptor NN 951 952 . . . 953 956 The the DT 957 964 nuclear nuclear JJ 965 971 factor factor NN 972 978 STAT-1 stat-1 NN 979 980 ( ( ( 980 983 p91 p91 NN 983 984 ) ) ) 985 988 was be VBD 989 1001 investigated investigate VBN 1002 1004 by by IN 1005 1024 immunoprecipitation immunoprecipitation NN 1025 1033 followed follow VBN 1034 1036 by by IN 1037 1051 immunoblotting immunoblotte VBG 1052 1055 for for IN 1056 1064 tyrosine tyrosine NN 1065 1080 phosphorylation phosphorylation NN 1080 1081 . . . 1082 1088 STAT-1 STAT-1 NNP 1089 1092 was be VBD 1093 1101 tyrosine tyrosine NN 1102 1116 phosphorylated phosphorylated JJ 1117 1123 within within IN 1124 1126 15 15 CD 1127 1130 min min NN 1131 1133 of of IN 1134 1138 IL-5 IL-5 NNP 1139 1150 stimulation stimulation NN 1150 1151 . . . 1152 1155 The the DT 1156 1164 presence presence NN 1165 1167 of of IN 1168 1174 STAT-1 stat-1 NN 1175 1177 in in IN 1178 1181 the the DT 1182 1189 nuclear nuclear JJ 1190 1197 extract extract NN 1198 1201 was be VBD 1202 1209 studied study VBN 1210 1212 by by IN 1213 1228 electrophoretic electrophoretic JJ 1229 1237 mobility mobility NN 1238 1243 shift shift NN 1244 1249 assay assay NN 1249 1250 . . . 1251 1255 IL-5 IL-5 NNP 1256 1263 induced induce VBD 1264 1267 two two CD 1268 1276 proteins protein NNS 1277 1281 that that WDT 1282 1287 bound bind VBD 1288 1290 to to TO 1291 1294 the the DT 1295 1311 gamma-activating gamma-activating JJ 1312 1320 sequence sequence NN 1320 1321 . . . 1322 1324 In in IN 1325 1328 the the DT 1329 1337 presence presence NN 1338 1340 of of IN 1341 1343 an an DT 1344 1355 anti-STAT-1 anti-stat-1 JJ 1356 1358 Ab Ab NNP 1358 1359 , , , 1360 1363 the the DT 1364 1368 band band NN 1369 1372 was be VBD 1373 1385 supershifted supershifte VBN 1385 1386 . . . 1387 1391 Thus thus RB 1391 1392 , , , 1393 1395 we we PRP 1396 1408 demonstrated demonstrate VBD 1409 1413 that that IN 1414 1418 IL-5 il-5 NN 1419 1428 activated activate VBD 1429 1432 the the DT 1433 1436 Jak Jak NNP 1437 1443 2-STAT 2-STAT NNP 1444 1445 1 1 CD 1446 1455 signaling signaling NN 1456 1463 pathway pathway NN 1464 1466 in in IN 1467 1478 eosinophils eosinophil NNS 1478 1479 . . . 1480 1482 We we PRP 1483 1492 speculate speculate VBP 1493 1497 that that IN 1498 1501 the the DT 1502 1505 Jak Jak NNP 1506 1512 2-STAT 2-STAT NNP 1513 1514 1 1 CD 1515 1522 pathway pathway NN 1523 1526 may may MD 1527 1529 be be VB 1530 1538 involved involve VBN 1539 1541 in in IN 1542 1545 the the DT 1546 1556 activation activation NN 1557 1559 of of IN 1560 1574 IL-5-inducible il-5-inducible JJ 1575 1580 genes gene NNS 1581 1583 in in IN 1584 1595 eosinophils eosinophil NNS 1595 1596 . . .