0 1 A a DT 2 11 conserved conserve VBN 12 17 motif motif NN 18 20 in in IN 21 24 the the DT 25 34 promoters promoter NNS 35 37 of of IN 38 45 several several JJ 46 55 cytokines cytokine NNS 56 65 expressed express VBN 66 68 by by IN 69 74 human human JJ 75 83 Th2-type th2-type JJ 84 95 lymphocytes lymphocyte NNS 95 96 . . . 98 100 We we PRP 101 105 have have VBP 106 114 recently recently RB 115 120 found find VBN 121 122 a a DT 123 128 novel novel JJ 129 138 conserved conserve VBN 139 144 motif motif NN 145 147 in in IN 148 151 the the DT 152 161 promoters promoter NNS 162 164 of of IN 165 172 several several JJ 173 189 T-cell-expressed t-cell-expressed JJ 190 199 cytokines cytokine NNS 200 201 [ [ ( 201 206 human human JJ 207 220 interleukin-2 interleukin-2 NN 220 221 , , , 222 224 -4 -4 CD 224 225 , , , 226 228 -5 -5 CD 229 232 and and CC 233 236 -13 -13 CD 237 240 and and CC 241 246 human human JJ 247 250 and and CC 251 256 mouse mouse NN 257 286 granulocyte/macrophage-colony granulocyte/macrophage-colony NN 287 298 stimulating stimulating NN 299 305 factor factor NN 306 307 ( ( ( 307 313 GM-CSF GM-CSF NNP 313 314 ) ) ) 314 315 ] ] ) 315 316 . . . 317 319 It it PRP 320 328 contains contain VBZ 329 330 a a DT 331 335 core core NN 336 344 sequence sequence NN 345 350 CTTGG cttgg NN 351 354 … ... : 355 360 CCAAG CCAAG NNP 361 366 which which WDT 367 369 is be VBZ 370 377 present present JJ 378 380 as as IN 381 385 part part NN 386 388 of of IN 389 395 larger large JJR 396 407 palindromic palindromic JJ 408 417 sequences sequence NNS 418 420 in in IN 421 425 each each DT 426 430 gene gene NN 430 431 . . . 432 436 This this DT 437 444 suggest suggest VBP 445 449 that that IN 450 454 they they PRP 455 458 may may MD 459 467 interact interact VB 468 472 with with IN 473 474 a a DT 475 478 new new JJ 479 485 family family NN 486 488 of of IN 489 501 trans-acting trans-acting JJ 502 509 factors factor NNS 509 510 . . . 511 513 In in IN 514 526 transfection transfection NN 527 533 assays assay NNS 533 534 , , , 535 538 the the DT 539 544 human human JJ 545 551 GM-CSF GM-CSF NNP 552 559 element element NN 560 563 has have VBZ 564 565 a a DT 566 572 strong strong JJ 573 581 positive positive JJ 582 588 effect effect NN 589 591 on on IN 592 595 the the DT 596 606 expression expression NN 607 609 of of IN 610 611 a a DT 612 620 reporter reporter NN 621 625 gene gene NN 626 628 by by IN 629 632 the the DT 633 638 human human JJ 639 640 T t NN 641 645 cell cell NN 646 650 line line NN 651 657 Jurkat Jurkat NNP 658 660 J6 J6 NNP 661 665 upon upon IN 666 677 stimulation stimulation NN 677 678 . . . 679 681 In in IN 682 685 DNA DNA NNP 686 694 mobility mobility NN 695 700 shift shift NN 701 707 assays assay NNS 707 708 , , , 709 713 this this DT 714 722 sequence sequence NN 723 726 can can MD 727 731 give give VB 732 738 either either CC 739 742 six six CD 743 752 different different JJ 753 761 specific specific JJ 762 767 bands band NNS 768 773 which which WDT 774 777 are be VBP 778 786 competed compete VBN 787 790 out out RP 791 793 by by IN 794 803 different different JJ 804 809 parts part NNS 810 812 of of IN 813 816 the the DT 817 825 sequence sequence NN 826 828 or or CC 829 832 one one CD 833 841 specific specific JJ 842 846 band band NN 847 852 which which WDT 853 855 is be VBZ 856 864 competed compete VBN 865 868 out out RP 869 871 by by IN 872 876 each each DT 877 879 of of IN 880 883 the the DT 884 892 inverted invert VBN 893 900 repeats repeat NNS 900 901 , , , 902 911 depending depend VBG 912 914 on on IN 915 918 the the DT 919 933 reconstitution reconstitution NN 934 944 conditions condition NNS 944 945 . . . 946 948 In in IN 949 958 different different JJ 959 964 genes gene NNS 964 965 , , , 966 969 the the DT 970 974 core core NN 975 984 sequences sequence NNS 985 988 are be VBP 989 998 separated separate VBN 999 1001 by by IN 1002 1009 integer integer NN 1010 1017 numbers number NNS 1018 1020 of of IN 1021 1028 helical helical JJ 1029 1034 turns turn NNS 1034 1035 . . . 1036 1047 Considering consider VBG 1048 1051 the the DT 1052 1058 strong strong JJ 1059 1067 positive positive JJ 1068 1078 regulatory regulatory JJ 1079 1085 effect effect NN 1086 1088 of of IN 1089 1093 this this DT 1094 1101 element element NN 1102 1105 and and CC 1106 1109 its its PRP$ 1110 1118 presence presence NN 1119 1121 in in IN 1122 1129 several several JJ 1130 1146 T-cell-expressed t-cell-expressed JJ 1147 1155 cytokine cytokine NN 1156 1161 genes gene NNS 1161 1162 , , , 1163 1165 it it PRP 1166 1169 may may MD 1170 1172 be be VB 1173 1180 crucial crucial JJ 1181 1183 to to TO 1184 1187 the the DT 1188 1199 coordinated coordinate VBN 1200 1210 expression expression NN 1211 1213 of of IN 1214 1219 these these DT 1220 1229 cytokines cytokine NNS 1230 1232 in in IN 1233 1234 T t NN 1235 1241 helper helper NN 1242 1247 cells cell NNS 1247 1248 . . .