0 3 The the DT 4 26 interleukin-5/receptor interleukin-5/receptor NN 27 38 interaction interaction NN 39 48 activates activate VBZ 49 52 Lyn Lyn NNP 53 56 and and CC 57 61 Jak2 jak2 NN 62 70 tyrosine tyrosine NN 71 78 kinases kinase NNS 79 82 and and CC 83 93 propagates propagate VBZ 94 101 signals signal NNS 102 105 via via IN 106 109 the the DT 110 123 Ras-Raf-1-MAP Ras-Raf-1-MAP NNP 124 130 kinase kinase NNP 131 134 and and CC 135 138 the the DT 139 147 Jak-STAT jak-stat JJ 148 156 pathways pathway NNS 157 159 in in IN 160 171 eosinophils eosinophil NNS 171 172 . . . 174 176 We we PRP 177 181 have have VBP 182 187 shown show VBN 188 192 that that IN 193 196 the the DT 197 208 interaction interaction NN 209 211 of of IN 212 223 interleukin interleukin NN 224 225 ( ( ( 225 230 IL)-5 IL)-5 NNP 231 235 with with IN 236 239 the the DT 240 248 receptor receptor NN 249 258 activates activate VBZ 259 262 Lyn Lyn NNP 263 271 tyrosine tyrosine NN 272 278 kinase kinase NNP 279 285 within within IN 286 287 1 1 CD 288 291 min min NN 292 295 and and CC 296 300 Jak2 jak2 NN 301 309 tyrosine tyrosine NN 310 316 kinase kinase NNP 317 323 within within IN 324 327 1-3 1-3 CD 328 332 min. min. NN 333 337 IL-5 IL-5 NNP 338 342 also also RB 343 353 stimulates stimulate VBZ 354 357 GTP GTP NNP 358 365 binding bind VBG 366 368 to to TO 369 375 p21ras p21ras NN 375 376 . . . 377 380 The the DT 381 387 signal signal NN 388 390 is be VBZ 391 403 subsequently subsequently RB 404 414 propagated propagate VBN 415 422 through through IN 423 426 the the DT 427 437 activation activation NN 438 440 of of IN 441 446 Raf-1 Raf-1 NNP 446 447 , , , 448 451 MEK MEK NNP 451 452 , , , 453 456 and and CC 457 460 MAP MAP NNP 461 468 kinases kinase NNS 469 471 as as IN 472 477 shown show VBN 478 480 by by IN 481 486 their their PRP$ 487 496 increased increase VBN 497 516 autophosphorylation autophosphorylation NN 517 519 in in FW 520 525 vitro vitro FW 526 529 and and CC 530 545 phosphorylation phosphorylation NN 546 548 in in FW 549 553 situ situ FW 553 554 . . . 555 559 Jak2 Jak2 NNP 560 566 kinase kinase NNP 567 570 has have VBZ 571 575 been be VBN 576 581 shown show VBN 582 584 to to TO 585 598 phosphorylate phosphorylate VB 599 603 STAT stat NN 604 611 nuclear nuclear JJ 612 620 proteins protein NNS 620 621 . . . 622 625 The the DT 626 636 activation activation NN 637 639 of of IN 640 644 STAT STAT NNP 645 652 nuclear nuclear JJ 653 660 factors factor NNS 661 664 was be VBD 665 672 studied study VBN 673 675 by by IN 676 691 electrophoretic electrophoretic JJ 692 700 mobility mobility NN 701 706 shift shift NN 707 712 assay assay NN 713 718 using use VBG 719 720 a a DT 721 726 gamma gamma NN 727 737 activation activation NN 738 742 site site NN 743 744 ( ( ( 744 747 GAS GAS NNP 747 748 ) ) ) 749 754 probe probe NN 754 755 . . . 756 758 We we PRP 759 764 found find VBD 765 769 that that IN 770 774 IL-5 IL-5 NNP 775 782 induces induce VBZ 783 786 two two CD 787 798 GAS-binding gas-binding JJ 799 807 proteins protein NNS 808 810 in in IN 811 822 eosinophils eosinophil NNS 822 823 , , , 824 827 one one CD 828 830 of of IN 831 836 which which WDT 837 839 is be VBZ 840 845 STAT1 stat1 NN 845 846 . . . 847 849 We we PRP 850 858 conclude conclude VBP 859 863 that that IN 864 868 IL-5 il-5 NN 869 876 induced induced JJ 877 884 signals signal NNS 885 888 are be VBP 889 899 propagated propagate VBN 900 907 through through IN 908 911 two two CD 912 920 distinct distinct JJ 921 929 pathways pathway NNS 929 930 : : : 931 932 ( ( ( 932 933 1 1 LS 933 934 ) ) ) 935 964 Lyn–>Ras–>Raf-1–>MEK–>MAP Lyn–>Ras–>Raf-1–>MEK–>MAP NNP 965 971 kinase kinase NNP 972 975 and and CC 976 977 ( ( ( 977 978 2 2 LS 978 979 ) ) ) 980 992 Jak2–>STAT1 Jak2–>STAT1 NNP 992 993 . . .